Article (Scientific journals)
Effects of Endocrine Disruptor Compounds, Alone or in Combination, on Human Macrophage-Like THP-1 Cell Response
Couleau, Nicolas; Falla, Jairo; Beillerot, Adeline et al.
2015In PLoS ONE, 10 (7), p. 0131428
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Keywords :
Cell differentiation; Secretion; Phagocytosis; Cytokines; Macrophages; Estrogens; Immune response
Abstract :
[en] The aim of the present study was to evaluate the immunological effects on human macrophages of four endocrine disruptor compounds (EDCs) using the differentiated human THP-1 cell line as a model. We studied first the effects of these EDCs, including Bisphenol A (BPA), di-ethylhexyl-phthalate (DEHP), dibutyl phthalate (DBP) and 4-tert-octylphenol (4-OP), either alone or in combination, on cytokine secretion, and phagocytosis. We then determined whether or not these effects were mediated by estrogen receptors via MAPK pathways. It was found that all four EDCs studied reduced strongly the phagocytosis of the differentiated THP-1 cells and that several of these EDCs disturbed also TNF-α, IL-1 β and IL-8 cytokine secretions. Furthermore, relative to control treatment, decreased ERK 1/2 phosphorylation was always associated with EDCs treatments-either alone or in certain combinations (at 0.1 μM for each condition). Lastly, as treatments by an estrogen receptor antagonist suppressed the negative effects on ERK 1/2 phosphorylation observed in cells treated either alone with BPA, DEHP, 4-OP or with the combined treatment of BPA and DEHP, we suggested that estrogen receptor-dependent pathway is involved in mediating the effects of EDCs on human immune system. Altogether, these results advocate that EDCs can disturb human immune response at very low concentrations.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Couleau, Nicolas;  Université de Lorraine, IUT Thionville-Yutz, Espace Cormontaigne, Yutz, France > CNRS UMR 7360 > Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)
Falla, Jairo;  Université de Lorraine, IUT Thionville-Yutz, Espace Cormontaigne, Yutz, France > CNRS UMR 7360 > Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)
Beillerot, Adeline;  IUT Thionville-Yutz, Impasse Alfred Kastler Espace Cormontaigne, Yutz, France
Battaglia, Eric;  Université de Lorraine, Campus Bridoux-8, Metz, France > CNRS UMR 7360 > Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)
d’Innocenzo, Marielle;  IUT Thionville-Yutz, Impasse Alfred Kastler Espace Cormontaigne, Yutz, France
PLANÇON, Sébastien ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Laval-Gilly, Philippe;  Université de Lorraine, IUT Thionville-Yutz, Espace Cormontaigne, Yutz, France > CNRS UMR 7360 > Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)
Bennasroune, Amar;  Université de Lorraine, IUT Thionville-Yutz, Espace Cormontaigne, Yutz, France > CNRS UMR 7360 > Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)
External co-authors :
yes
Language :
English
Title :
Effects of Endocrine Disruptor Compounds, Alone or in Combination, on Human Macrophage-Like THP-1 Cell Response
Publication date :
02 July 2015
Journal title :
PLoS ONE
ISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
Volume :
10
Issue :
7
Pages :
e0131428
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 11 May 2017

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