Reference : Mitogenic signals control translation of the early growth response gene-1 in myogenic...
Scientific journals : Article
Human health sciences : Cardiovascular & respiratory systems
Mitogenic signals control translation of the early growth response gene-1 in myogenic cells.
Maass, A. [> >]
Grohe, C. [> >]
Oberdorf, S. [> >]
Sukhatme, V. P. [> >]
Vetter, H. [> >]
Neyses, Ludwig mailto [University of Luxembourg > Research Office]
Biochemical and biophysical research communications
[en] Angiotensin II/pharmacology ; Animals ; Base Sequence ; Cells, Cultured ; DNA-Binding Proteins/genetics/metabolism ; Early Growth Response Protein 1 ; Endothelins/pharmacology ; Fibroblast Growth Factor 2/pharmacology ; Immediate-Early Proteins ; Insulin/pharmacology ; Mice ; Molecular Sequence Data ; Muscles/cytology/drug effects/metabolism ; Oligodeoxyribonucleotides ; Phenylephrine/pharmacology ; Platelet-Derived Growth Factor/pharmacology ; Protein Biosynthesis ; Proto-Oncogene Proteins c-sis ; Signal Transduction ; Tetradecanoylphorbol Acetate/pharmacology ; Transcription Factors/genetics/metabolism ; Transforming Growth Factor beta/pharmacology ; Zinc Fingers/genetics
[en] Muscle is a major site of expression of the early growth response gene-1 (Egr-1). To investigate its role in muscle proliferation and/or differentiation we studied the effect of a variety of growth factors on cultured mouse muscle Sol8 cells. Three groups of responses could be distinguished: 1. AII, endothelin, phenylephrine, and PMA induced Egr-1 mRNA accumulation, but the message remained untranslated. These factors induced neither differentiation nor proliferation. 2. Insulin induced differentiation. It stimulated Egr-1 mRNA accumulation, but no translation into the Egr-1 protein was seen. 3. bFGF, PDGF BB, and FCS strongly induced DNA- and protein synthesis (i.e. proliferation) and Egr-1 mRNA accumulation. Only under these conditions was the message translated into protein. We conclude: 1. AII, endothelin, phenylephrine, and PMA elicit a nuclear response in Sol8 muscle cells which may lead to reprogramming of genes unrelated to differentiation or proliferation. 2. Differentiation induces a translational block of the Egr-1 mRNA which is only relieved by mitotic stimuli. 3. These results strongly suggest a pivotal role of Egr-1 in muscle proliferation and define translational control as a new mechanism of Egr-1 regulation.

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