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Article (Scientific journals)
Inhibition of endothelin-1 induced myocardial protein synthesis by an antisense oligonucleotide against the early growth response gene-1.
NEYSES, Ludwig; Nouskas, J.; Vetter, H.
1991In Biochemical and biophysical research communications, 181 (1), p. 22-7
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Keywords :
Animals; Cells, Cultured; DNA-Binding Proteins/genetics; Early Growth Response Protein 1; Endothelins/antagonists & inhibitors/pharmacology; Genes/drug effects; Heart/drug effects/physiology; Immediate-Early Proteins; Kinetics; Myocardium/metabolism; Oligonucleotides, Antisense/pharmacology; Phenylalanine/metabolism; Protein Biosynthesis; RNA, Messenger/genetics; Rats; Rats, Inbred WKY; Transcription Factors/genetics; Zinc Fingers/genetics
Abstract :
[en] We explored the role of the recently discovered "early growth response gene-1 (Egr-1)" in the induction of myocardial protein synthesis by endothelin-1. Endothelin-1 stimulated protein synthesis (i.e. 3H-phenylalanine incorporation) in isolated adult rat cardiomyocytes more than 2-fold. Addition of a 15mer Egr-1 antisense oligodeoxyribonucleotide complementary to the first 5 codons of the Egr-1 mRNA completely blocked endothelin-induced protein synthesis. A single base mismatch in the oligonucleotide sequence abolished the inhibitory effect. T3-induced stimulation of protein synthesis was unaffected by the antisense oligonucleotide. These results indicate that the Egr-1 gene product is involved (putatively as a third messenger) in the signal transduction cascade initiated by endothelin-1 which eventually culminates in the induction of cardiac protein synthesis.
Disciplines :
Cardiovascular & respiratory systems
Author, co-author :
NEYSES, Ludwig ;  University of Luxembourg > Research Office
Nouskas, J.
Vetter, H.
External co-authors :
yes
Language :
English
Title :
Inhibition of endothelin-1 induced myocardial protein synthesis by an antisense oligonucleotide against the early growth response gene-1.
Publication date :
1991
Journal title :
Biochemical and biophysical research communications
ISSN :
0006-291X
Volume :
181
Issue :
1
Pages :
22-7
Peer reviewed :
Peer reviewed
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