| Reference : Inhibition of endothelin-1 induced myocardial protein synthesis by an antisense oligo... |
| Scientific journals : Article | |||
| Human health sciences : Cardiovascular & respiratory systems | |||
| http://hdl.handle.net/10993/27497 | |||
| Inhibition of endothelin-1 induced myocardial protein synthesis by an antisense oligonucleotide against the early growth response gene-1. | |
| English | |
Neyses, Ludwig  [University of Luxembourg > Research Office] | |
| Nouskas, J. [> >] | |
| Vetter, H. [> >] | |
| 1991 | |
| Biochemical and biophysical research communications | |
| 181 | |
| 1 | |
| 22-7 | |
| Yes | |
| International | |
| 0006-291X | |
| UNITED STATES | |
| [en] Animals ; Cells, Cultured ; DNA-Binding Proteins/genetics ; Early Growth Response Protein 1 ; Endothelins/antagonists & inhibitors/pharmacology ; Genes/drug effects ; Heart/drug effects/physiology ; Immediate-Early Proteins ; Kinetics ; Myocardium/metabolism ; Oligonucleotides, Antisense/pharmacology ; Phenylalanine/metabolism ; Protein Biosynthesis ; RNA, Messenger/genetics ; Rats ; Rats, Inbred WKY ; Transcription Factors/genetics ; Zinc Fingers/genetics | |
| [en] We explored the role of the recently discovered "early growth response gene-1 (Egr-1)" in the induction of myocardial protein synthesis by endothelin-1. Endothelin-1 stimulated protein synthesis (i.e. 3H-phenylalanine incorporation) in isolated adult rat cardiomyocytes more than 2-fold. Addition of a 15mer Egr-1 antisense oligodeoxyribonucleotide complementary to the first 5 codons of the Egr-1 mRNA completely blocked endothelin-induced protein synthesis. A single base mismatch in the oligonucleotide sequence abolished the inhibitory effect. T3-induced stimulation of protein synthesis was unaffected by the antisense oligonucleotide. These results indicate that the Egr-1 gene product is involved (putatively as a third messenger) in the signal transduction cascade initiated by endothelin-1 which eventually culminates in the induction of cardiac protein synthesis. | |
| http://hdl.handle.net/10993/27497 | |
| 10.1016/S0006-291X(05)81376-2 | |
| http://www.sciencedirect.com/science/article/pii/S0006291X05813762 |
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