Article (Scientific journals)
Gonadotropin-releasing hormone neuropeptides and receptor in human breast cancer: Correlation to poor prognosis parameters
Pazaitou-Panayiotou, Kalliopi; Chemonidou, Christina; Poupi, Aliki et al.
2013In Peptides, 42, p. 15-24
Peer reviewed
 

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Keywords :
Breast; Cancer; Gonadotropin releasing hormone; Human; Neuropeptide; Receptor; Adenocarcinoma; Aged; Biopsy; Breast Neoplasms; Cell Line, Tumor; Female; Gene Expression Regulation, Neoplastic; Gonadotropin-Releasing Hormone; Humans; Middle Aged; Prognosis; Protein Precursors; Receptors, LHRH; Reference Values
Abstract :
[en] Expression of the two gonadotropin-releasing hormone homologue peptides GnRHI and GnRHII and their receptor GnRHR has been demonstrated in a number of malignancies. In hormone-dependent breast cancer, GnRH analogs are used for therapy in premenopausal women. Gene expression of GnRHI, II and R was studied in breast biopsies from primary breast adenocarcinoma obtained from the tumor and the adjacent benign tissue. Levels were evaluated by a multiplex real-time RT-PCR. GnRHI transcripts were detected in 14.7% of the benign and 29.4% malignant biopsies and GnRHII in 21.2% benign and 44.1% malignant biopsies. GnRHR was also more frequent in the malignant (54.2%) than in the benign (24.0%) biopsies, at similar expression levels. No transcripts were detected in biopsies from healthy individuals. There was a strong correlation between the presence of GnRHI and GnRHII transcripts and their receptor in the benign and the malignant biopsies. GnRHI, II and R expression correlated significantly with poor prognosis pathological parameters. Immunohistochemistry for GnRHR revealed expression in malignant cells and in epithelial cells of mammary ducts of the adjacent area with pre-cancerous features. In contrast, GnRH I and II peptides were rarely expressed at low levels in breast cancer cells. In conclusion GnRH peptides and receptor are expressed more frequently in breast tumors than in the adjacent mammary tissue, representing a malignant feature. Their expression correlated to tumor characteristics of poor prognosis and was therefore related to more aggressive malignancies. Concomitant expression of peptides and receptor supports an autocrine/paracrine regulating role. © 2012 Elsevier Inc.
Disciplines :
Biochemistry, biophysics & molecular biology
Identifiers :
eid=2-s2.0-84877312517
Author, co-author :
Pazaitou-Panayiotou, Kalliopi;  Department of Endocrinology-Endocrine Oncology, Theagenio Cancer Hospital, Thessaloniki, Greece
Chemonidou, Christina;  Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
Poupi, Aliki;  Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
Koureta, Maria;  Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
Kaprara, Athina;  Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
Lambropoulou, Maria;  Laboratory of Histology-Embryology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
Constantinidis, Theodoros C.;  Laboratory of Hygiene and Environmental Protection, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
Galaktidou, Grammati;  Department of Endocrinology-Endocrine Oncology, Theagenio Cancer Hospital, Thessaloniki, Greece
Koffa, Maria ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Kiziridou, Anastasia;  Department of Endocrinology-Endocrine Oncology, Theagenio Cancer Hospital, Thessaloniki, Greece
Kakolyris, Stylianos;  Department of Oncology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
Kolios, George;  Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
Kortsaris, Alexandros;  Laboratory of Biochemistry, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
Chatzaki, Ekaterini;  Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece, Laboratory of Pharmacology, DUTH, Dragana, Alexandroupolis 68100, Thrace, Greece
More authors (4 more) Less
External co-authors :
yes
Language :
English
Title :
Gonadotropin-releasing hormone neuropeptides and receptor in human breast cancer: Correlation to poor prognosis parameters
Publication date :
2013
Journal title :
Peptides
ISSN :
0196-9781
Volume :
42
Pages :
15-24
Peer reviewed :
Peer reviewed
Available on ORBilu :
since 28 May 2016

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