Reference : CK2 phosphorylation of C/EBPdelta regulates its transcription factor activity.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
CK2 phosphorylation of C/EBPdelta regulates its transcription factor activity.
Schwind, Lisa [> >]
Zimmer, Andreas David mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit]
Gotz, Claudia [> >]
Montenarh, Mathias [> >]
The international journal of biochemistry & cell biology
Yes (verified by ORBilu)
[en] Amino Acid Sequence ; CCAAT-Enhancer-Binding Protein-delta/genetics/metabolism ; Casein Kinase II/antagonists & inhibitors/genetics/metabolism ; Catalytic Domain ; Conserved Sequence ; HCT116 Cells ; Humans ; Molecular Sequence Data ; Phosphorylation ; Protein Interaction Domains and Motifs ; Protein Kinase Inhibitors/pharmacology ; Transcriptome ; Phosphorylation ; Protein kinase CK2 ; Protein-protein interaction ; Transcription ; Transcription factor
[en] Protein kinase CK2 plays an essential role in cell viability in lower and higher eukaryotes. As a global regulator it phosphorylates and thereby regulates a broad array of cellular targets including a large number of transcription factors. Here, we have identified the CCAAT/enhancer binding protein delta (C/EBPdelta) as a new substrate for CK2. Using point mutants of C/EBPdelta the major phosphorylation site for CK2 was mapped to serine 57, which is located within the transactivation domain of C/EBPdelta. For proper functioning as a transcription factor C/EBPdelta has to be translocated into the nucleus where it forms heterodimers with other members of the C/EBP family of proteins and ATF4. Here, we found that CK2 phosphorylation does neither influence the subcellular localization of C/EBPdelta nor its interaction with C/EBPbeta, but rather does CK2 phosphorylation modulate the transcriptional activity of C/EBPdelta. Moreover, we found that CK2 bound to C/EBPdelta, which might help to target CK2 to the transcriptional machinery where it can phosphorylate other transcription factors or co-activators.
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