Article (Scientific journals)
A microtubule-independent role of p150glued in secretory cargo concentration at endoplasmic reticulum exit sites
Verissimo, Fatima; HALAVATYI, Aliaksandr; Pepperkok, Rainer et al.
2015In Journal of Cell Science, 128 (22), p. 4160-4170
Peer reviewed
 

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Keywords :
Endoplasmic reticulum; ER exit sites; FRAP; Microtubules; Modeling; Protein sorting; Secretory pathway; Article; Golgi complex; HeLa cell line
Abstract :
[en] Newly synthesized proteins are sorted into COPII-coated transport carriers at the endoplasmic reticulum (ER). Assembly of the COPII coat complex, which occurs at ER exit sites (ERES), is initiated by membrane association and GTP loading of SAR1, followed by the recruitment of the SEC23-SEC24 and SEC13-SEC31 subcomplexes. Both of these two subcomplexes stimulate GTP hydrolysis and coat disassembly. This inherent disassembly capacity of COPII complexes needs to be regulated to allow sufficient time for cargo sorting and transport carrier formation. By performing fluorescence recovery after photobleaching (FRAP) and mathematical modeling, we show that p150glued (also known as DCTN1), a component of the dynactin complex, stabilizes the COPII pre-budding complex on ER membranes in a microtubule-independent manner. Concentration of the secretory marker ts-O45-G at ERES is reduced in the presence of a C-terminal p150glued fragment that prevents binding of endogenous p150glued to SEC23. A similar cargo reduction is observed upon p150glued knockdown. Taken together, our data suggest that cargo concentration at ERES is regulated by p150glued to coordinate protein sorting and transport carrier formation with the subsequent long-range transport towards the Golgi complex along microtubules. © 2015. Published by The Company of Biologists Ltd.
Disciplines :
Microbiology
Identifiers :
eid=2-s2.0-84949883161
Author, co-author :
Verissimo, Fatima;  Cell Biology and Biophysics Unit, EMBL, Meyerhofstraße 1, Heidelberg, Germany
HALAVATYI, Aliaksandr ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Pepperkok, Rainer;  Cell Biology and Biophysics Unit, EMBL, Meyerhofstraße 1, Heidelberg, Germany
Weiss, Matthias;  Experimental Physics I, University of Bayreuth, Universitaetsstr. 30, Bayreuth, Germany
External co-authors :
yes
Language :
English
Title :
A microtubule-independent role of p150glued in secretory cargo concentration at endoplasmic reticulum exit sites
Publication date :
2015
Journal title :
Journal of Cell Science
ISSN :
0021-9533
Publisher :
Company of Biologists Ltd
Volume :
128
Issue :
22
Pages :
4160-4170
Peer reviewed :
Peer reviewed
Funders :
RGY0076/2010, HFSP, Human Frontier Science Program
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