Article (Scientific journals)
Decoding the dynamics of cellular metabolism and the action of 3-bromopyruvate and 2-deoxyglucose using pulsed stable isotope-resolved metabolomics
Mudrich Epouse Dorosz, Susann Antonia; Pietzke, Matthias; Zasada, Christin
2014In Cancer and Metabolism
Peer reviewed
 

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Keywords :
Stable isotope labeling; GC-MS; Cancer metabolism; Glycolysis; Metabolomics; 3-Bromopyruvate; 2-Deoxyglucose
Abstract :
[en] Background Cellular metabolism is highly dynamic and continuously adjusts to the physiological program of the cell. The regulation of metabolism appears at all biological levels: (post-) transcriptional, (post-) translational, and allosteric. This regulatory information is expressed in the metabolome, but in a complex manner. To decode such complex information, new methods are needed in order to facilitate dynamic metabolic characterization at high resolution. Results Here, we describe pulsed stable isotope-resolved metabolomics (pSIRM) as a tool for the dynamic metabolic characterization of cellular metabolism. We have adapted gas chromatography-coupled mass spectrometric methods for metabolomic profiling and stable isotope-resolved metabolomics. In addition, we have improved robustness and reproducibility and implemented a strategy for the absolute quantification of metabolites. Conclusions By way of examples, we have applied this methodology to characterize central carbon metabolism of a panel of cancer cell lines and to determine the mode of metabolic inhibition of glycolytic inhibitors in times ranging from minutes to hours. Using pSIRM, we observed that 2-deoxyglucose is a metabolic inhibitor, but does not directly act on the glycolytic cascade.
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
Mudrich Epouse Dorosz, Susann Antonia ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Pietzke, Matthias
Zasada, Christin
External co-authors :
yes
Language :
English
Title :
Decoding the dynamics of cellular metabolism and the action of 3-bromopyruvate and 2-deoxyglucose using pulsed stable isotope-resolved metabolomics
Publication date :
2014
Journal title :
Cancer and Metabolism
Peer reviewed :
Peer reviewed
Available on ORBilu :
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