Reference : Modulation of Pleurodeles waltl DNA Polymerase mu Expression by Extreme Conditions En...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
Modulation of Pleurodeles waltl DNA Polymerase mu Expression by Extreme Conditions Encountered during Spaceflight
Schenten, Véronique [Stress Immunity Pathogens Laboratory, EA7300, Lorraine University, Vandœuvre-lès-Nancy, France]
Gueguinou, Nathan mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit]
Baatout, Sarah [Radiobiology Unit, Belgian Nuclear Research Centre, SCKCEN, Mol, Belgium, Department of Molecular Biotechnology, Ghent University, Ghent, Belgium]
Frippiat, Jean-Pol [Stress Immunity Pathogens Laboratory, EA7300, Lorraine University, Vandœuvre-lès-Nancy, France]
Yes (verified by ORBilu)
[en] DNA directed DNA polymerase mu ; DNA repair ; Pleurodeles ; Pleurodeles waltl ; Salamandridae ; VDJ recombination ; Animals ; Blotting, Western ; Circadian Rhythm ; DNA, Complementary ; DNA-Directed DNA Polymerase ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Enzymologic ; Larva ; Male ; Molecular Sequence Data ; Oxidation-Reduction ; Phylogeny ; Pleurodeles ; Protein Carbonylation ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA ; Space Flight ; Testis ; Transcriptome
[en] DNA polymerase μ is involved in DNA repair, V(D)J recombination and likely somatic hypermutation of immunoglobulin genes. Our previous studies demonstrated that spaceflight conditions affect immunoglobulin gene expression and somatic hypermutation frequency. Consequently, we questioned whether Polμ expression could also be affected. To address this question, we characterized Polμ of the Iberian ribbed newt Pleurodeles waltl and exposed embryos of that species to spaceflight conditions or to environmental modifications corresponding to those encountered in the International Space Station. We noted a robust expression of Polμ mRNA during early ontogenesis and in the testis, suggesting that Polμ is involved in genomic stability. Full-length Polμ transcripts are 8-9 times more abundant in P. waltl than in humans and mice, thereby providing an explanation for the somatic hypermutation predilection of G and C bases in amphibians. Polμ transcription decreases after 10 days of development in space and radiation seem primarily involved in this down-regulation. However, space radiation, alone or in combination with a perturbation of the circadian rhythm, did not affect Polμ protein levels and did not induce protein oxidation, showing the limited impact of radiation encountered during a 10-day stay in the International Space Station. © 2013 Schenten et al.

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