Reference : Two novel regulators of N-acetyl-galactosamine utilization pathway and distinct roles...
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
Two novel regulators of N-acetyl-galactosamine utilization pathway and distinct roles in bacterial infections
Zhang, Huimin []
Ravcheev, Dmitry mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Hu, Dan []
Zhang, Fengyu []
Gong, Xiufang []
Hao, Lina []
Cao, Min []
Rodionov, Dmitry A. []
Wang, Changjun []
Feng, Youjun []
John Wiley & Sons
[en] AgaR ; amino sugars ; d-galactosamine ; N-acetyl-d-galactosamine ; Streptococcus suis ; virulence
[en] Bacterial pathogens can exploit metabolic pathways to facilitate their successful
infection cycles, but little is known about roles of d-galactosamine
(GalNAc) catabolism pathway in bacterial pathogenesis.
Here, we report the genomic reconstruction of GalN/GalNAc utilization pathway
in Streptococci and the diversified aga regulons. We delineated two new paralogous
AgaR regulators for the GalN/GalNAc catabolism pathway. The electrophoretic
mobility shift assays experiment demonstrated that AgaR2 (AgaR1)
binds the predicted palindromes, and the combined in vivo data from reverse
transcription quantitative polymerase chain reaction and RNA-seq
that AgaR2 (not AgaR1) can effectively repress the transcription of the target
genes. Removal of agaR2 (not agaR1) from Streptococcus suis 05ZYH33 augments
significantly the abilities of both adherence to Hep-2
cells and anti-phagocytosis
against RAW264.7 macrophage. As anticipated, the dysfunction in AgaR2-mediated
regulation of S. suis impairs its pathogenicity in experimental models
of both mice and piglets. Our finding discovered two novel regulators specific
for GalN/GalNAc catabolism and assigned them distinct roles into bacterial
infections. To the best of our knowledge, it might represent a first paradigm
that links the GalN/GalNAc catabolism pathway to bacterial pathogenesis.
Luxembourg Centre for Systems Biomedicine (LCSB): Molecular Systems Physiology (Thiele Group)

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