Available on ORBilu since
30 April 2016
Article (Scientific journals)
The hematopoietic factor granulocyte-colony stimulating factor improves outcome in experimental spinal cord injury.
Pitzer, Claudia; Klussmann, Stefan; Krüger, Carola et al.
2010In Journal of Neurochemistry, 113, p. 930-942
Peer Reviewed verified by ORBi


Full Text
The hematopoietic factor granulocyte-colony factor improves outcome in experimental spinal cord injury.docx
Author preprint (14.18 kB)

All documents in ORBilu are protected by a user license.

Send to


Keywords :
Granulocyte-colony stimulating factor (G-CSF); spinal cord injury.
Abstract :
[en] Granulocyte-colony stimulating factor (G-CSF) is a potent hematopoietic factor that drives differentiation of neutrophilic granulocytes. We have recently shown that G-CSF also acts as a neuronal growth factor, protects neurons in vitro and in vivo, and has regenerative potential in various neurological disease models. Spinal cord injury (SCI) following trauma or secondary to skeletal instability is a terrible condition with no effective therapies available at present. In this study, we show that the G-CSF receptor is up-regulated upon experimental SCI and that G-CSF improves functional outcome in a partial dissection model of SCI. G-CSF significantly decreases apoptosis in an experimental partial spinal transsection model in the mouse and increases expression of the anti-apoptotic G-CSF target gene Bcl-X(L). In vitro, G-CSF enhances neurite outgrowth and branching capacity of hippocampal neurons. In vivo, G-CSF treatment results in improved functional connectivity of the injured spinal cord as measured by Mn(2+)-enhanced MRI. G-CSF also increased length of the dorsal corticospinal tract and density of serotonergic fibers cranial to the lesion center. Mice treated systemically with G-CSF as well as transgenic mice over-expressing G-CSF in the CNS exhibit a strong improvement in functional outcome as measured by the BBB score and gridwalk analysis. We show that G-CSF improves outcome after experimental SCI by counteracting apoptosis, and enhancing connectivity in the injured spinal cord. We conclude that G-CSF constitutes a promising and feasible new therapy option for SCI.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Pitzer, Claudia;  Sygnis Bioscience
Klussmann, Stefan;  German Cancer Research Center > Department of Molecular Neurobiology
Krüger, Carola;  Sygnis Bioscience
Letellier, Elisabeth ;  German Cancer Research Center
Plaas, Christian;  Sygnis Bioscience
Dittgen, Tanjew;  Sygnis Bioscience
Kirsch, Friederike;  Sygnis Bioscience
Stieltjes, Bram;  German Cancer Research Center > Department of Radiology
Weber, Daniela;  Sygnis Bioscience
Laage, Rico;  Sygnis Bioscience
Martin-Villalba, Ana;  German Cancer Research Center > Department of Molecular Neurobiology
Schneider, Armin;  Sygnis Bioscience
External co-authors :
Language :
Title :
The hematopoietic factor granulocyte-colony stimulating factor improves outcome in experimental spinal cord injury.
Publication date :
May 2010
Journal title :
Journal of Neurochemistry
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
Pages :
Peer reviewed :
Peer Reviewed verified by ORBi
Focus Area :
Systems Biomedicine


Number of views
82 (2 by Unilu)
Number of downloads
10 (1 by Unilu)

Scopus citations®
Scopus citations®
without self-citations
WoS citations


Similar publications

Contact ORBilu