Article (Scientific journals)
Temperature and nitric oxide control spontaneous calcium transients in astrocytes.
Schipke, Carola G.; Heidemann, Antje; Skupin, Alexander et al.
2008In Cell Calcium, 43 (3), p. 285-95
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Keywords :
Animals; Astrocytes/cytology/drug effects/metabolism; Calcium Signaling/drug effects; Cells, Cultured; Cerebral Cortex/cytology/drug effects/metabolism; Mice; Nitric Oxide/pharmacology; Nitric Oxide Donors/pharmacology; Perfusion; S-Nitrosoglutathione/pharmacology; Temperature; Time Factors
Abstract :
[en] Transient spontaneous increases in the intracellular Ca2+ concentration have been frequently observed in astrocytes in cell culture and in acutely isolated slices from several brain regions. Recent in vivo experiments, however, reported only a low frequency of spontaneous Ca2+ events in astrocytes. Since the ex vivo experiments were usually performed at temperatures lower than physiological body temperature, we addressed the question whether temperature could influence the spontaneous Ca2+ activity in astrocytes. Indeed, comparing the frequency and spike width of spontaneous Ca2+ transients in astrocytes at temperatures between 20 and 37 degrees C in culture as well as in acute cortical slices from mouse brain, revealed that spontaneous Ca2+ responses occurred frequently at low temperature and became less frequent at higher temperature. Moreover, the single Ca2+ events had a longer duration at low temperature. We found that nitric oxide (NO) mimicked the increase in spontaneous Ca2+ activity and that an NO-synthase inhibitor attenuated the effect of lowering the temperature. Thus, temperature and NO are major determinants of spontaneous astrocytic Ca2+ signalling.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Schipke, Carola G.
Heidemann, Antje
Skupin, Alexander  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Peters, Oliver
Falcke, Martin
Kettenmann, Helmut
External co-authors :
yes
Language :
English
Title :
Temperature and nitric oxide control spontaneous calcium transients in astrocytes.
Publication date :
2008
Journal title :
Cell Calcium
ISSN :
0143-4160
Publisher :
Churchill Livingstone, United States
Volume :
43
Issue :
3
Pages :
285-95
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 03 November 2015

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