Article (Scientific journals)
Aneuploidy underlies a multicellular phenotypic switch.
Tan, Zhihao; Hays, Michelle; Cromie, Gareth A. et al.
2013In Proceedings of the National Academy of Sciences of the United States of America, 110 (30), p. 12367-72
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Keywords :
Aneuploidy; Chromosomes, Fungal; Gene Dosage; Phenotype; Saccharomyces cerevisiae/genetics; bet-hedging; colony morphology; copy number variation; phenotypic switching
Abstract :
[en] Although microorganisms are traditionally used to investigate unicellular processes, the yeast Saccharomyces cerevisiae has the ability to form colonies with highly complex, multicellular structures. Colonies with the "fluffy" morphology have properties reminiscent of bacterial biofilms and are easily distinguished from the "smooth" colonies typically formed by laboratory strains. We have identified strains that are able to reversibly toggle between the fluffy and smooth colony-forming states. Using a combination of flow cytometry and high-throughput restriction-site associated DNA tag sequencing, we show that this switch is correlated with a change in chromosomal copy number. Furthermore, the gain of a single chromosome is sufficient to switch a strain from the fluffy to the smooth state, and its subsequent loss to revert the strain back to the fluffy state. Because copy number imbalance of six of the 16 S. cerevisiae chromosomes and even a single gene can modulate the switch, our results support the hypothesis that the state switch is produced by dosage-sensitive genes, rather than a general response to altered DNA content. These findings add a complex, multicellular phenotype to the list of molecular and cellular traits known to be altered by aneuploidy and suggest that chromosome missegregation can provide a quick, heritable, and reversible mechanism by which organisms can toggle between phenotypes.
Research center :
- Luxembourg Centre for Systems Biomedicine (LCSB): Integrative Cell Signalling (Skupin Group)
Luxembourg Centre for Systems Biomedicine (LCSB): Experimental Neurobiology (Balling Group)
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Tan, Zhihao
Hays, Michelle
Cromie, Gareth A.
Jeffery, Eric W.
Scott, Adrian C.
Ahyong, Vida
Sirr, Amy
Skupin, Alexander  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Dudley, Aimee M.
External co-authors :
Language :
Title :
Aneuploidy underlies a multicellular phenotypic switch.
Publication date :
Journal title :
Proceedings of the National Academy of Sciences of the United States of America
Publisher :
National Academy of Sciences, Washington, United States - District of Columbia
Volume :
Issue :
Pages :
Peer reviewed :
Peer Reviewed verified by ORBi
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