Article (Scientific journals)
The plasma membrane calcium ATPase modulates calcium homeostasis, intracellular signaling events and function in platelets.
Jones, S.; Solomon, A.; Sanz-Rosa, D. et al.
2010In Journal of Thrombosis and Haemostasis, 8 (12), p. 2766-74
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Keywords :
Animals; Blood Platelets/enzymology/metabolism; Blotting, Western; Calcium/metabolism; Calcium-Transporting ATPases/metabolism; Homeostasis; Male; Membrane Proteins/metabolism; Mice; Mice, Inbred BALB C; Phosphorylation; Platelet Aggregation; Signal Transduction
Abstract :
[en] BACKGROUND: The plasma membrane calcium ATPase (PMCA) regulates localized signaling events in a variety of cell types, although its functional role in platelets remains undefined. OBJECTIVES: To investigate the role of PMCA in determining platelet intracellular calcium concentration ([Ca(2)(+) ](i) ) at rest and following agonist stimulation, and to define the corresponding effects upon different stages of platelet activation. METHODS: [Ca(2)(+) ](i) was continuously measured in Fura-2-loaded platelets and in vitro and in vivo functional analyses performed in the presence of the PMCA inhibitor carboxyeosin (CE). RESULTS: Concentrations of CE that selectively inhibited Ca(2)(+) extrusion through PMCA were established in human platelets. [Ca(2)(+) ](i) was elevated by CE in resting platelets, although collagen-stimulated Ca(2)(+) release was reduced. Impaired Ca(2)(+) mobilization upon agonist stimulation was accompanied by reduced dense granule secretion and impaired platelet aggregation. Platelet aggregation responses were also reduced in PMCA4(-/-) mice and in an in vivo mouse model of platelet thromboembolism. Conversely, inhibition of PMCA promoted the early and later stages of platelet activation, observed as enhanced adhesion to fibrinogen, and accelerated clot retraction. Investigations into the signaling mechanisms underlying CE-mediated inhibition of platelet aggregation implicated cGMP-independent vasodilator-stimulated phosphoprotein phosphorylation. CONCLUSIONS: Disruption of PMCA activity perturbs platelet Ca(2)(+) homeostasis and function in a time-dependent manner, demonstrating that PMCA differentially regulates Ca(2)(+) -dependent signaling events, and hence function, throughout the platelet activation process.
Disciplines :
Cardiovascular & respiratory systems
Author, co-author :
Jones, S.
Solomon, A.
Sanz-Rosa, D.
Moore, C.
Holbrook, L.
Cartwright, E. J.
Neyses, Ludwig ;  University of Luxembourg > Research Office
Emerson, M.
Language :
English
Title :
The plasma membrane calcium ATPase modulates calcium homeostasis, intracellular signaling events and function in platelets.
Publication date :
2010
Journal title :
Journal of Thrombosis and Haemostasis
ISSN :
1538-7836
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
8
Issue :
12
Pages :
2766-74
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
(c) 2010 International Society on Thrombosis and Haemostasis.
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