Keywords :
Animals; Blotting, Western; Cell Division/drug effects; Cell Line; DNA-Binding Proteins/biosynthesis/genetics; Early Growth Response Protein 1; Estrogens/metabolism/pharmacology/physiology; Gene Expression Regulation/drug effects; Immediate-Early Proteins; Mice; Muscle Development; Muscle, Skeletal/cytology/drug effects/growth & development; Rats; Receptors, Estrogen/metabolism/physiology; Transcription Factors/biosynthesis/genetics; Transcriptional Activation
Abstract :
[en] To determine the role of estrogen in skeletal muscle growth, we investigated estrogen receptor-mediated effects on proliferation in skeletal myoblasts. In L6, C2C12 and Sol8 myoblasts estrogen receptor was demonstrated by immunoblotting, immunofluorescence microscopy and transfection studies. Estrone induced a significant increase in myoblast growth whereas 17 beta-estradiol had no effect. Furthermore in L6-cells estrone (c-fos: 3.9-fold, egr-1: 4.6-fold) induced immediate-early gene induction significantly stronger than 17 beta-estradiol (c-fos: 1.7-fold, egr-1: 2.3-fold; p < 0.05). Skeletal myoblasts express functional estrogen receptors. Estrogens differ in the activation of skeletal myoblast growth and immediate-early gene induction.
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