Reference : Overexpression of sarcolemmal calcium pump attenuates induction of cardiac gene expre...
Scientific journals : Article
Human health sciences : Cardiovascular & respiratory systems
Overexpression of sarcolemmal calcium pump attenuates induction of cardiac gene expression in response to ET-1.
Piuhola, J. [> >]
Hammes, A. [> >]
Schuh, K. [> >]
Neyses, Ludwig mailto [University of Luxembourg > Research Office]
Vuolteenaho, O. [> >]
Ruskoaho, H. [> >]
American journal of physiology. Regulatory, integrative and comparative physiology
Yes (verified by ORBilu)
United States
[en] Adrenomedullin ; Animals ; Animals, Genetically Modified ; Atrial Natriuretic Factor/genetics/metabolism/secretion ; Calcium-Transporting ATPases/biosynthesis/genetics ; Caveolae/metabolism ; Cell Membrane/metabolism ; Endothelin-1/pharmacology ; Gene Expression Regulation/drug effects ; Heart Ventricles/drug effects/metabolism ; Hemodynamics/physiology ; Humans ; Myocardial Contraction/drug effects/physiology ; Myocardium/metabolism ; Natriuretic Peptide, Brain ; Peptides/genetics/metabolism ; Perfusion ; Proto-Oncogene Proteins c-fos/genetics/metabolism ; RNA, Messenger/metabolism ; Rats ; Sarcolemma/metabolism ; Signal Transduction/drug effects/physiology ; Stress, Mechanical
[en] The function of the plasma membrane calmodulin-dependent calcium ATPase (PMCA) in myocardium is unknown. PMCA is localized in caveolae, 50- to 100-nm membrane invaginations, which also contain receptors for endothelin-1 (ET-1) and various other ligands. PMCA has been suggested to play a role in regulation of caveolar signal transduction. We studied the effects of the hypertrophic agonist ET-1 and increased coronary perfusion pressure on cardiac synthesis of B-type natriuretic peptide (BNP) in transgenic rats overexpressing the human PMCA 4CI in isolated perfused heart preparation. ET-1 infusion for 2 h increased BNP mRNA levels twofold in left ventricles (LV) of nontransgenic rats, whereas no increase was noted in PMCA rat hearts. Similar responses were seen in adrenomedullin and c-fos mRNA levels, and in immunoreactive BNP secretion. Increased mechanical load produced by elevated perfusion pressure induced similar 1.5- to 1.6-fold increases in LV BNP mRNA in both nontransgenic and PMCA rat hearts. These results show that cardiac overexpression of PMCA attenuates ET-1-stimulated early induction of cardiac gene expression, suggesting that PMCA may modulate myocardial growth responses.

File(s) associated to this reference

Fulltext file(s):

Limited access
Overexpression of sarcolemmal calcium pump attenuates induction of cardiac gene expression in response to ET-1..pdfPublisher postprint186.12 kBRequest a copy

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.