Article (Scientific journals)
Targeted deletion of the extracellular signal-regulated protein kinase 5 attenuates hypertrophic response and promotes pressure overload-induced apoptosis in the heart.
Kimura, Tomomi E.; Jin, Jiawei; Zi, Min et al.
2010In Circulation Research, 106 (5), p. 961-70
Peer Reviewed verified by ORBi
 

Files


Full Text
Target Deletion of the Extracellular Signal-Regulated Protein Kinase 5 Attenautes Hypertrophic Response.pdf
Author postprint (1.93 MB)
Download

All documents in ORBilu are protected by a user license.

Send to



Details



Keywords :
Aniline Compounds/pharmacology; Animals; Animals, Newborn; Apoptosis/drug effects; Blood Pressure; Cardiomegaly/enzymology/genetics/pathology/physiopathology/prevention & control; Cells, Cultured; Fibrosis; Hypertension/enzymology/genetics/pathology/physiopathology; Indoles/pharmacology; Male; Mice; Mice, Knockout; Mitogen-Activated Protein Kinase 7/antagonists & inhibitors/deficiency/genetics; Mutation; Myocytes, Cardiac/drug effects/enzymology/pathology; Myogenic Regulatory Factors/genetics/metabolism; Protein Kinase Inhibitors/pharmacology; RNA Interference; Rats; Time Factors; Transcription, Genetic; Transfection; Ventricular Remodeling/drug effects
Abstract :
[en] RATIONALE: Mitogen-activated protein kinase (MAPK) pathways provide a critical connection between extrinsic and intrinsic signals to cardiac hypertrophy. Extracellular signal-regulated protein kinase (ERK)5, an atypical MAPK is activated in the heart by pressure overload. However, the role of ERK5 plays in regulating hypertrophic growth and hypertrophy-induced apoptosis is not completely understood. OBJECTIVE: Herein, we investigate the in vivo role and signaling mechanism whereby ERK5 regulates cardiac hypertrophy and hypertrophy-induced apoptosis. METHODS AND RESULTS: We generated and examined the phenotypes of mice with cardiomyocyte-specific deletion of the erk5 gene (ERK5(cko)). In response to hypertrophic stress, ERK5(cko) mice developed less hypertrophic growth and fibrosis than controls. However, increased apoptosis together with upregulated expression levels of p53 and Bad were observed in the mutant hearts. Consistently, we found that silencing ERK5 expression or specific inhibition of its kinase activity using BIX02189 in neonatal rat cardiomyocytes (NRCMs) reduced myocyte enhancer factor (MEF)2 transcriptional activity and blunted hypertrophic responses. Furthermore, the inhibition of MEF2 activity in NRCMs using a non-DNA binding mutant form of MEF2 was found to attenuate the ERK5-regulated hypertrophic response. CONCLUSIONS: These results reveal an important function of ERK5 in cardiac hypertrophic remodeling and cardiomyocyte survival. The role of ERK5 in hypertrophic remodeling is likely to be mediated via the regulation of MEF2 activity.
Disciplines :
Cardiovascular & respiratory systems
Author, co-author :
Kimura, Tomomi E.
Jin, Jiawei
Zi, Min
Prehar, Sukhpal
Liu, Wei
Oceandy, Delvac
Abe, Jun-Ichi
NEYSES, Ludwig ;  University of Luxembourg > Research Office
Weston, Arthur H.
Cartwright, Elizabeth J.
Wang, Xin
Language :
English
Title :
Targeted deletion of the extracellular signal-regulated protein kinase 5 attenuates hypertrophic response and promotes pressure overload-induced apoptosis in the heart.
Publication date :
2010
Journal title :
Circulation Research
ISSN :
0009-7330
eISSN :
1524-4571
Publisher :
Lippincott Williams & Wilkins, United States - Maryland
Volume :
106
Issue :
5
Pages :
961-70
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 14 August 2014

Statistics


Number of views
121 (2 by Unilu)
Number of downloads
210 (0 by Unilu)

Scopus citations®
 
64
Scopus citations®
without self-citations
54
OpenAlex citations
 
83
WoS citations
 
62

Bibliography


Similar publications



Contact ORBilu