Patent (Patents)
Method and kit for the isolation of genomic dna, rna, proteins and metabolites from a single biological sample
Wilmes, Paul
2014
 

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Abstract :
[en] The invention provides method and kit for the separation and purification of cellular components including polar and non-polar metabolites, genomic DNA, RNA and proteins from a single biological sample where two steps of lysis of the cells are performed sequentially, before and after a metabolite isolation step. The first lysis step is mechanical and performed in order to be incomplete, whereas the second is chemical or both mechanical and chemical. A sequential isolation of genomic DNA, RNA and proteins is carried out after the second lysis step.
Disciplines :
Microbiology
Inventor :
Wilmes, Paul ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Language :
English
Title (54) :
Method and kit for the isolation of genomic dna, rna, proteins and metabolites from a single biological sample
Publication date (43-45) :
07 March 2014
Filing date (22) :
02 August 2012
Patent/publication number (10-11) :
WO2013029919 A1
Patent status (13) :
A
Priority number (30) :
LU20110091864 20110902
International classification (IPC) (51) :
C12N15/10
European classification (ECLA) (52) :
C12N15/1003, C12N1/066, C12N15/101
Applicant (71) :
Université Du Luxembourg, Centre De Recherche Public - Gabriel Lippmann
Publication organism (19) :
WIPO
Name of the research project :
ATTRACT Research Project Systems Biology of Natural Microbial Assemblages
Funders :
FNR - Fonds National de la Recherche [LU]
Available on ORBilu :
since 15 May 2014

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