[en] The invention provides method and kit for the separation and purification of cellular components including polar and non-polar metabolites, genomic DNA, RNA and proteins from a single biological sample where two steps of lysis of the cells are performed sequentially, before and after a metabolite isolation step. The first lysis step is mechanical and performed in order to be incomplete, whereas the second is chemical or both mechanical and chemical. A sequential isolation of genomic DNA, RNA and proteins is carried out after the second lysis step.
Disciplines :
Microbiologie
Inventeur :
WILMES, Paul ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Langue du document :
Anglais
Titre (54) :
Method and kit for the isolation of genomic dna, rna, proteins and metabolites from a single biological sample
Date de publication (43-45) :
07 mars 2014
Date de dépôt (22) :
02 août 2012
N° de brevet/publication (10-11) :
WO2013029919 A1
Etat de la demande (13) :
A
N° de priorité (30) :
LU20110091864 20110902
Classification internationale (IPC) (51) :
C12N15/10
Classification européenne (ECLA) (52) :
C12N15/1003, C12N1/066, C12N15/101
Déposant (71) :
Université Du Luxembourg, Centre De Recherche Public - Gabriel Lippmann
Organisme de publication du brevet (19) :
WIPO
Intitulé du projet de recherche :
ATTRACT Research Project Systems Biology of Natural Microbial Assemblages
