Article (Scientific journals)
HIV-1 Vpu's lipid raft association is dispensable for counteraction of the particle release restriction imposed by CD317/Tetherin.
Fritz, Joëlle; Tibroni, Nadine; Keppler, Oliver T. et al.
2012In Virology, 424 (1), p. 33-44
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Keywords :
Amino Acid Motifs; Amino Acid Sequence; Antigens, CD/genetics/metabolism; Cell Line; Cell Membrane/metabolism/virology; GPI-Linked Proteins/antagonists & inhibitors/genetics/metabolism; HIV Infections/genetics/metabolism/virology; HIV-1/genetics/physiology; Human Immunodeficiency Virus Proteins/chemistry/genetics/metabolism; Membrane Lipids/metabolism; Molecular Sequence Data; Protein Binding; Sequence Alignment; Viral Regulatory and Accessory Proteins/chemistry/genetics/metabolism; Virus Release
Abstract :
[en] HIV-1 Vpu antagonizes the block to particle release mediated by CD317 (BST-2/HM1.24/Tetherin) via incompletely understood mechanisms. Vpu and CD317 partially reside in cholesterol-rich lipid rafts where HIV-1 budding preferentially occurs. Here we find that lipid raft association of ectopically expressed or endogenous CD317 was unaltered upon co-expression with Vpu or following HIV-1 infection. Similarly, Vpu's lipid raft association remained unchanged upon expression of CD317. We identify amino acids V25 and Y29 of Vpu as crucial for microdomain partitioning and single substitution of these amino acids resulted in Vpu variants with markedly reduced or undetectable lipid raft association. These mutations did not affect Vpu's subcellular distribution and binding capacity to CD317, nor its ability to downmodulate cell surface CD317 and promote HIV-1 release from CD317-positive cells. We conclude that (i) lipid raft incorporation is dispensable for Vpu-mediated CD317 antagonism and (ii) Vpu does not antagonize CD317 by extraction from lipid rafts.
Disciplines :
Immunology & infectious disease
Author, co-author :
Fritz, Joëlle 
Tibroni, Nadine
Keppler, Oliver T.
Fackler, Oliver T.
Language :
English
Title :
HIV-1 Vpu's lipid raft association is dispensable for counteraction of the particle release restriction imposed by CD317/Tetherin.
Publication date :
2012
Journal title :
Virology
ISSN :
1096-0341
Publisher :
Elsevier, Atlanta, United States - California
Volume :
424
Issue :
1
Pages :
33-44
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2011 Elsevier Inc. All rights reserved.
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since 14 May 2013

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