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Article (Scientific journals)
Reliable recovery of inbred mouse lines using cryopreserved spermatozoa.
Marschall, S.; Huffstadt, U.; BALLING, Rudi et al.
1999In Mammalian Genome, 10 (8), p. 773-6
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Keywords :
Animal Husbandry/methods; Animals; Crosses, Genetic; Cryopreservation; Ethylnitrosourea/toxicity; Female; Fertilization in Vitro; Humans; Male; Mice; Mice, Inbred C3H; Mice, Inbred Strains/genetics; Mice, Mutant Strains; Mutagens/toxicity; Reproducibility of Results; Semen Preservation
Abstract :
[en] Since the mouse has become the most detailed model system to investigate the genetics and pathogenesis of human diseases, large numbers of new mouse strains have and continue to be produced. In nearly all animal facilities, the maintenance of breeding colonies is limited and mouse strains have to be archived in an efficient way. This study was undertaken to test the reliability of recovering mouse lines by use of cryopreserved spermatozoa from individual male mice. In contrast to many studies, spermatozoa and oocytes were derived from the same genetic background. 30 C3HeB/FeJ males belonging to three different categories (wild-type, F1-generation of ENU-treated males, and defined mutants) were recovered by producing at least 20 offspring from each donor. Independent of the experimental group, every single male was successfully recovered. Archiving mouse strains by cryopreservation of spermatozoa may, therefore, offer a reliable way to preserve genetically valuable mouse strains and provides an efficient management strategy for animal facilities.
Disciplines :
Genetics & genetic processes
Author, co-author :
Marschall, S.
Huffstadt, U.
BALLING, Rudi 
Hrabe de Angelis, M.
Language :
English
Title :
Reliable recovery of inbred mouse lines using cryopreserved spermatozoa.
Publication date :
1999
Journal title :
Mammalian Genome
ISSN :
0938-8990
eISSN :
1432-1777
Publisher :
Springer, Germany
Volume :
10
Issue :
8
Pages :
773-6
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 10 May 2013

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