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Article (Scientific journals)
Expanded metabolic reconstruction of Helicobacter pylori (iIT341 GSM/GPR): an in silico genome-scale characterization of single- and double-deletion mutants.
THIELE, Ines; Vo, Thuy D.; Price, Nathan D. et al.
2005In Journal of Bacteriology, 187 (16), p. 5818-30
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Keywords :
Biotin/biosynthesis; Energy Metabolism/genetics; Gene Deletion; Genes, Lethal; Genome, Bacterial; Helicobacter pylori/genetics/growth & development/metabolism; Models, Genetic; Mutagenesis; Phenotype; Reproducibility of Results
Abstract :
[en] Helicobacter pylori is a human gastric pathogen infecting almost half of the world population. Herein, we present an updated version of the metabolic reconstruction of H. pylori strain 26695 based on the revised genome annotation and new experimental data. This reconstruction, iIT341 GSM/GPR, represents a detailed review of the current literature about H. pylori as it integrates biochemical and genomic data in a comprehensive framework. In total, it accounts for 341 metabolic genes, 476 intracellular reactions, 78 exchange reactions, and 485 metabolites. Novel features of iIT341 GSM/GPR include (i) gene-protein-reaction associations, (ii) elementally and charge-balanced reactions, (iii) more accurate descriptions of isoprenoid and lipopolysaccharide metabolism, and (iv) quantitative assessments of the supporting data for each reaction. This metabolic reconstruction was used to carry out in silico deletion studies to identify essential and conditionally essential genes in H. pylori. A total of 128 essential and 75 conditionally essential metabolic genes were identified. Predicted growth phenotypes of single knockouts were validated using published experimental data. In addition, in silico double-deletion studies identified a total of 47 synthetic lethal mutants involving 67 different metabolic genes in rich medium.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
THIELE, Ines 
Vo, Thuy D.
Price, Nathan D.
Palsson, Bernhard O.
Language :
English
Title :
Expanded metabolic reconstruction of Helicobacter pylori (iIT341 GSM/GPR): an in silico genome-scale characterization of single- and double-deletion mutants.
Publication date :
2005
Journal title :
Journal of Bacteriology
ISSN :
1098-5530
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
Volume :
187
Issue :
16
Pages :
5818-30
Peer reviewed :
Peer Reviewed verified by ORBi
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since 12 December 2013

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