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A quantitative approach to catabolite repression in Escherichia coli.
Bettenbrock, Katja; Fischer, Sophia; Kremling, Andreas et al.
2006In Journal of Biological Chemistry, 281 (5), p. 2578-84
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Keywords :
Carbohydrate Metabolism; Escherichia coli/genetics/metabolism; Escherichia coli Proteins/metabolism; Kinetics; Models, Biological; Mutation; Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism; Phosphorylation; Signal Transduction
Abstract :
[en] A dynamic mathematical model was developed to describe the uptake of various carbohydrates (glucose, lactose, glycerol, sucrose, and galactose) in Escherichia coli. For validation a number of isogenic strains with defined mutations were used. By considering metabolic reactions as well as signal transduction processes influencing the relevant pathways, we were able to describe quantitatively the phenomenon of catabolite repression in E. coli. We verified model predictions by measuring time courses of several extra- and intracellular components such as glycolytic intermediates, EII-ACrr phosphorylation level, both LacZ and PtsG concentrations, and total cAMP concentrations under various growth conditions. The entire data base consists of 18 experiments performed with nine different strains. The model describes the expression of 17 key enzymes, 38 enzymatic reactions, and the dynamic behavior of more than 50 metabolites. The different phenomena affecting the phosphorylation level of EIIACrr, the key regulation molecule for inducer exclusion and catabolite repression in enteric bacteria, can now be explained quantitatively.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Bettenbrock, Katja
Fischer, Sophia
Kremling, Andreas
Jahreis, Knut
Sauter, Thomas ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Gilles, Ernst-Dieter
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Title :
A quantitative approach to catabolite repression in Escherichia coli.
Publication date :
Journal title :
Journal of Biological Chemistry
Publisher :
American Society for Biochemistry and Molecular Biology, United States - Maryland
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Peer reviewed :
Peer Reviewed verified by ORBi
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