No full text
Article (Scientific journals)
STAT3 is enriched in nuclear bodies.
Herrmann, Andreas; Sommer, Ulrike; Pranada, Albert L. et al.
2004In Journal of Cell Science, 117 (Pt 2), p. 339-49
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Animals; COS Cells; CREB-Binding Protein; Cell Nucleus/genetics/metabolism; Cercopithecus aethiops; Chromatin/metabolism; DNA-Binding Proteins/genetics/metabolism; Fluorescence Recovery After Photobleaching; Gene Expression Regulation/physiology; Histones/metabolism; Interleukin-6/metabolism; Nuclear Proteins/metabolism; Phosphorylation; Recombinant Fusion Proteins/metabolism; STAT3 Transcription Factor; SUMO-1 Protein/metabolism; Signal Transduction; Trans-Activators/genetics/metabolism; Transcription, Genetic/genetics
Abstract :
[en] Signal transducer and activator of transcription 3 (STAT3) is a transcription factor that is involved in a variety of biological functions. It is essential for the signal transduction of interleukin-6 (IL-6) and related cytokines. In response to IL-6 stimulation STAT3 becomes phosphorylated and translocates into the nucleus where it binds to enhancer sequences of target genes. We found that activated STAT3 is enriched in dot-like structures within the nucleus, which we termed STAT3 nuclear bodies. To examine the dynamics of STAT3 nuclear body formation, a fusion protein of STAT3 and yellow fluorescent protein (YFP) was constructed. Studies in living cells have shown that the appearance of STAT3 nuclear bodies is transient, correlating with the timecourse of tyrosine-phosphorylation of STAT3. Furthermore, we show by fluorescence recovery after photobleaching (FRAP) analysis that STAT3 within nuclear bodies consists of a highly mobile and an immobile fraction. Colocalization studies provided evidence that these bodies are accompanied with CREB binding protein (CBP) and acetylated histone H4, which are markers for transcriptionally active chromatin. Moreover, STAT3 nuclear bodies in HepG2 cells are not colocalized with promyelocytic leukemia oncoprotein (PML)-containing bodies; neither is a sumoylation of activated STAT3 detectable. Taken together, our data suggest that STAT3 nuclear bodies are either directly involved in active gene transcription or they serve as reservoirs of activated STAT3.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Herrmann, Andreas
Sommer, Ulrike
Pranada, Albert L.
Giese, Bernd
Kuster, Andrea
Haan, Serge ;  Rheinisch - Westfälische Technische Hochschule Aachen - RWTH > Institute for Biochemistry
Becker, Walter
Heinrich, Peter C.
Muller-Newen, Gerhard
External co-authors :
yes
Language :
English
Title :
STAT3 is enriched in nuclear bodies.
Publication date :
2004
Journal title :
Journal of Cell Science
ISSN :
1477-9137
Publisher :
The Company of Biologists, United Kingdom
Volume :
117
Issue :
Pt 2
Pages :
339-49
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
FULL TEXT available via http://jcs.biologists.org/content/117/2/339.long
Available on ORBilu :
since 26 April 2013

Statistics


Number of views
59 (12 by Unilu)
Number of downloads
0 (0 by Unilu)

Scopus citations®
 
51
Scopus citations®
without self-citations
44
OpenCitations
 
51
WoS citations
 
54

Bibliography


Similar publications



Contact ORBilu