Reference : Selective differentiation and proliferation of human eosinophils from umbilical cord ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Selective differentiation and proliferation of human eosinophils from umbilical cord blood: calcium fluxes and superoxide ion secretion
Zardini, D. M. [> >]
Tschirhart, Eric mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
EDP Sciences
Yes (verified by ORBilu)
Les Ulis
[en] pharmacology ; Cells, Cultured ; Eosinophils ; Female ; Fetal Blood ; Humans ; Infant, Newborn ; Interleukin-3 ; Interleukin-5 ; Kinetics ; N-Formylmethionine Leucyl-Phenylalanine ; Platelet Activating Factor ; Pregnancy ; Superoxides ; blood ; cytology ; drug effects ; physiology ; Calcium
[en] Investigation of the physiologic mechanisms involved in the activation of eosinophils is crucial to comprehend their role in the pathogenesis of allergic reactions. To overcome the difficulty of obtaining large numbers of eosinophils, we differentiated in vitro eosinophils from human umbilical cord blood mononuclear cells. These cells responded to fMLP or PAF with an increase in [Ca2+]i, associated with O2 production. Deprivation or chelation of extracellular calcium induced a reduction of fMLP or PAF-induced [Ca2+]i rise and O2- production. Similar results were obtained with extracellular Ni2+ addition. Chelation of intracellular calcium induced an inhibition of fMLP- or PAF-induced [Ca2+]i rise and a decrease in O2- production. Our results indicate that fMLP- and PAF-dependent O2- production in eosinophils requires intra- and extracellular Ca2+ and that Ca2+ influx is necessary for optimal activation.

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