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Article (Scientific journals)
Sequence analysis of the nucleocapsid gene of measles virus isolates from South Africa identifies a new genotype
Kreis, Stephanie; Vardas, E.; Whistler, T.
1997In Journal of General Virology, 78 (7), p. 1581-1587
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Keywords :
amino acid substitution; article; carboxy terminal sequence; genotype; measles virus; nonhuman; phylogeny; polymerase chain reaction; priority journal; rna sequence; south africa; viral genetics; virus nucleocapsid; Amino Acid Sequence; Base Sequence; Cell Line; DNA; Viral; Genotype; Humans; Measles virus; Molecular Sequence Data; Nucleocapsid; Phylogeny; Sequence Analysis; DNA; Sequence Homology; Amino Acid; South Africa; Measles virus
Abstract :
[en] Sequence analysis was performed on 20 measles virus (MV) isolates from South Africa, five of which were obtained between 1986 and 1989 and 15 isolates collected during the 1994/95 measles season. A 590 bp fragment of the carboxyl terminus of the nucleocapsid (N) was amplified by PCR and subjected to sequence and phylogenetic analysis. Comparison of the South African MV strains with those previously described revealed that at least two distinct groups of wild-type (wt) MV exist, one of which has been circulating since 1986. The major genotype (I) was represented by the more recent isolates which showed three characteristic amino acid substitutions. Furthermore, three vaccine-like viruses with sequences very similar to the Edmonston wt strain were identified. Phylogenetic analysis of 100 MV strains allowed the assignment of new definitions for MV genotypes and subgroups. Employing these definitions, the majority of South African isolates analysed here formed a new genotype.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Kreis, Stephanie ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Vardas, E.
Whistler, T.
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Title :
Sequence analysis of the nucleocapsid gene of measles virus isolates from South Africa identifies a new genotype
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Journal title :
Journal of General Virology
Publisher :
Society for General Microbiology, London, United Kingdom
Volume :
Issue :
Pages :
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
cited By (since 1996)52 <br />Scopus
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