| Reference : Responses of the coastal bacterial community to viral infection of the algae Phaeocys... |
| Scientific journals : Article | |||
| Life sciences : Aquatic sciences & oceanology Life sciences : Biochemistry, biophysics & molecular biology Life sciences : Environmental sciences & ecology Life sciences : Microbiology | |||
| http://hdl.handle.net/10993/4917 | |||
| Responses of the coastal bacterial community to viral infection of the algae Phaeocystis globosa | |
| English | |
Sheik, Abdul  [Max Planck Insitute for Marine Microbiology and Luxembourg Centre for Systems Biomedicine (LCSB)] | |
| Brussaard, Corina [] | |
| Lavik, Gaute [] | |
| Lam, Phyllis [] | |
| Musat, Niculina [] | |
| Krupke, Andreas [] | |
| Littmann, Sten [] | |
| Strous, Marc [] | |
| Kuypers, Marcel [] | |
| 2013 | |
| ISME Journal (The) | |
| Yes | |
| International | |
| 1751-7362 | |
| 1751-7370 | |
| [en] Alteromonas and Roseobacter ; carbon remineralisation ; nanoSIMS; Phaeocystis globosa; pyrosequencing; marine viruses | |
| [en] The release of organic material upon algal cell lyses has a key role in structuring bacterial communities and affects the cycling of biolimiting elements in the marine environment. Here we show that already before cell lysis the leakage or excretion of organic matter by infected yet intact algal cells shaped North Sea bacterial community composition and enhanced bacterial substrate assimilation. Infected algal cultures of Phaeocystis globosa grown in coastal North Sea water contained gamma- and alphaproteobacterial phylotypes that were distinct from those in the noninfected control cultures 5 h after infection. The gammaproteobacterial population at this time mainly consisted of Alteromonas sp. cells that were attached to the infected but still intact host cells. Nano-scale secondary-ion mass spectrometry (nanoSIMS) showed about 20% transfer of organic matter derived from the infected 13C- and 15N-labelled P. globosa cells to Alteromonas sp. cells. Subsequent, viral lysis of P. globosa resulted in the formation of aggregates that were densely colonised by bacteria. Aggregate dissolution was observed after 2 days, which we attribute to bacteriophage-induced lysis of the attached bacteria. Isotope mass spectrometry analysis showed
that 40% of the particulate 13C-organic carbon from the infected P. globosa culture was remineralizedto dissolved inorganic carbon after 7 days. These findings reveal a novel role of viruses in the leakage or excretion of algal biomass upon infection, which provides an additional ecological niche for specific bacterial populations and potentially redirects carbon availability. | |
| Researchers ; Professionals ; Students ; General public ; Others | |
| http://hdl.handle.net/10993/4917 | |
| 10.1038/ismej.2013.135 | |
| Authors retain copyright
Published source must be acknowledged and DOI cited Must link to publisher version Publisher's version/PDF cannot be used On author's personal website and institutional repository If funding agency rules apply, authors may post authors version to their relevant funding body's archive, 6 months after publication |
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