Article (Scientific journals)
λ-DNA Through porous materials – Surface Enhanced Raman Scattering in a simple plasmonic nanopore
Hubarevich, Aliaksandr; Huang, Jian-An; Giovannini, Giorgia et al.
2020In Journal of Physical Chemistry. C, Nanomaterials and interfaces, 124 (41), p. 22663-22670
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Abstract :
[en] Engineered electromagnetic fields in plasmonic nanopores enable enhanced optical detection for single molecule sensing and sequencing. Here, a plasmonic nanopore prepared in a thick nanoporous film is used to investigate, by means of surface enhanced Raman spectroscopy, the interaction between the metallic surface of the pore and a long-chain double strand DNA molecule free to diffuse through the pore. We discuss how the matrix of the porous material can interact with the molecule thanks to: i) transient aspecific interactions between the porous surface and DNA; ii) diffusion; iii) thermal and optical forces exerted by the localized field in a metallic nanostructure on the DNA molecule. An interaction time up to tens of milliseconds enables to collect high signal-to-noise Raman signatures, allowing an easy label-free reading of information from the DNA molecule. Moreover, to increase the rate of detection, we tested a polymeric porous hydrogel placed beneath the solid-state membrane. The hydrogel enables a slowdown of the molecule diffusion time, thus increasing the number of detected interaction events by a factor 20. The analysis of the observed Raman peaks and their relative intensities, combined with theoretical simulations, allow to get further information on the process of translocation and on the folding state and orientation of the translocating molecule. Our results demonstrate temporary adsorption of the DNA molecule on the porous material during the translocation due to the diffusion force. Finally, we provide a qualitative evaluation of the nucleotides’ contents in the different groups of collected signal. The proposed approach can find interesting applications not only in DNA sensing and sequencing, but also on generic nanopore spectroscopy.
Disciplines :
Physics
Author, co-author :
Hubarevich, Aliaksandr
Huang, Jian-An
Giovannini, Giorgia
Schirato, Andrea
Zhao, Yingqi
Maccaferri, Nicolò ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit
De Angelis, Francesco
Alabastri, Alessandro
Garoli, Denis
External co-authors :
yes
Language :
English
Title :
λ-DNA Through porous materials – Surface Enhanced Raman Scattering in a simple plasmonic nanopore
Publication date :
2020
Journal title :
Journal of Physical Chemistry. C, Nanomaterials and interfaces
ISSN :
1932-7455
Publisher :
American Chemical Society, Washington, United States - District of Columbia
Volume :
124
Issue :
41
Pages :
22663-22670
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 25 September 2020

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