Reference : λ-DNA Through porous materials – Surface Enhanced Raman Scattering in a simple plasmo...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Physics
http://hdl.handle.net/10993/44354
λ-DNA Through porous materials – Surface Enhanced Raman Scattering in a simple plasmonic nanopore
English
Hubarevich, Aliaksandr []
Huang, Jian-An []
Giovannini, Giorgia []
Schirato, Andrea []
Zhao, Yingqi []
Maccaferri, Nicolò mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit >]
De Angelis, Francesco []
Alabastri, Alessandro []
Garoli, Denis []
2020
Journal of Physical Chemistry C
American Chemical Society
124
41
22663-22670
Yes (verified by ORBilu)
International
1932-7447
1932-7455
Washington
DC
[en] Engineered electromagnetic fields in plasmonic nanopores enable enhanced optical detection for single molecule sensing and sequencing. Here, a plasmonic nanopore prepared in a thick nanoporous film is used to investigate, by means of surface enhanced Raman spectroscopy, the interaction between the metallic surface of the pore and a long-chain double strand DNA molecule free to diffuse through the pore. We discuss how the matrix of the porous material can interact with the molecule thanks to: i) transient aspecific interactions between the porous surface and DNA; ii) diffusion; iii) thermal and optical forces exerted by the localized field in a metallic nanostructure on the DNA molecule. An interaction time up to tens of milliseconds enables to collect high signal-to-noise Raman signatures, allowing an easy label-free reading of information from the DNA molecule. Moreover, to increase the rate of detection, we tested a polymeric porous hydrogel placed beneath the solid-state membrane. The hydrogel enables a slowdown of the molecule diffusion time, thus increasing the number of detected interaction events by a factor 20. The analysis of the observed Raman peaks and their relative intensities, combined with theoretical simulations, allow to get further information on the process of translocation and on the folding state and orientation of the translocating molecule. Our results demonstrate temporary adsorption of the DNA molecule on the porous material during the translocation due to the diffusion force. Finally, we provide a qualitative evaluation of the nucleotides’ contents in the different groups of collected signal. The proposed approach can find interesting applications not only in DNA sensing and sequencing, but also on generic nanopore spectroscopy.
http://hdl.handle.net/10993/44354
10.1021/acs.jpcc.0c06165

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Limited access
10.1021@acs.jpcc.0c06165.pdfAuthor postprint770.49 kBRequest a copy

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.