Article (Scientific journals)
Early Pleistocene enamel proteome from Dmanisi resolves Stephanorhinus phylogeny.
Cappellini, Enrico; Welker, Frido; Pandolfi, Luca et al.
2019In Nature
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Abstract :
[en] The sequencing of ancient DNA has enabled the reconstruction of speciation, migration and admixture events for extinct taxa(1). However, the irreversible post-mortem degradation(2) of ancient DNA has so far limited its recovery-outside permafrost areas-to specimens that are not older than approximately 0.5 million years (Myr)(3). By contrast, tandem mass spectrometry has enabled the sequencing of approximately 1.5-Myr-old collagen type I(4), and suggested the presence of protein residues in fossils of the Cretaceous period(5)-although with limited phylogenetic use(6). In the absence of molecular evidence, the speciation of several extinct species of the Early and Middle Pleistocene epoch remains contentious. Here we address the phylogenetic relationships of the Eurasian Rhinocerotidae of the Pleistocene epoch(7-9), using the proteome of dental enamel from a Stephanorhinus tooth that is approximately 1.77-Myr old, recovered from the archaeological site of Dmanisi (South Caucasus, Georgia)(10). Molecular phylogenetic analyses place this Stephanorhinus as a sister group to the clade formed by the woolly rhinoceros (Coelodonta antiquitatis) and Merck's rhinoceros (Stephanorhinus kirchbergensis). We show that Coelodonta evolved from an early Stephanorhinus lineage, and that this latter genus includes at least two distinct evolutionary lines. The genus Stephanorhinus is therefore currently paraphyletic, and its systematic revision is needed. We demonstrate that sequencing the proteome of Early Pleistocene dental enamel overcomes the limitations of phylogenetic inference based on ancient collagen or DNA. Our approach also provides additional information about the sex and taxonomic assignment of other specimens from Dmanisi. Our findings reveal that proteomic investigation of ancient dental enamel-which is the hardest tissue in vertebrates(11), and is highly abundant in the fossil record-can push the reconstruction of molecular evolution further back into the Early Pleistocene epoch, beyond the currently known limits of ancient DNA preservation.
Research center :
ULHPC - University of Luxembourg: High Performance Computing
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Cappellini, Enrico
Welker, Frido
Pandolfi, Luca
Ramos-Madrigal, Jazmin
Samodova, Diana
Ruther, Patrick L.
Fotakis, Anna K.
Lyon, David
Moreno-Mayar, J. Victor
Bukhsianidze, Maia
Rakownikow Jersie-Christensen, Rosa
Mackie, Meaghan
Ginolhac, Aurélien  ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Ferring, Reid
Tappen, Martha
Palkopoulou, Eleftheria
Dickinson, Marc R.
Stafford, Thomas W. Jr
Chan, Yvonne L.
Gotherstrom, Anders
Nathan, Senthilvel K. S. S.
Heintzman, Peter D.
Kapp, Joshua D.
Kirillova, Irina
Moodley, Yoshan
Agusti, Jordi
Kahlke, Ralf-Dietrich
Kiladze, Gocha
Martinez-Navarro, Bienvenido
Liu, Shanlin
Sandoval Velasco, Marcela
Sinding, Mikkel-Holger S.
Kelstrup, Christian D.
Allentoft, Morten E.
Orlando, Ludovic
Penkman, Kirsty
Shapiro, Beth
Rook, Lorenzo
Dalen, Love
Gilbert, M. Thomas P.
Olsen, Jesper V.
Lordkipanidze, David
Willerslev, Eske
More authors (33 more) Less
External co-authors :
yes
Language :
English
Title :
Early Pleistocene enamel proteome from Dmanisi resolves Stephanorhinus phylogeny.
Publication date :
2019
Journal title :
Nature
ISSN :
1476-4687
Publisher :
Nature Publishing Group, London, United Kingdom
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 17 September 2019

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