Reference : A model of yeast glycolysis based on a consistent kinetic characterisation of all its...

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Multidisciplinary, general & others
	To cite this reference:	http://hdl.handle.net/10993/3833

Title :	A model of yeast glycolysis based on a consistent kinetic characterisation of all its enzymes
Language :	English
Author, co-author :	Smallbone, Kieran* []
	Messiha, Hanan L.* []
	Carroll, Kathleen M.* []
	Winder, Catherine L.* []
	Malys, Naglis* []
	Dunn, Warwick B. []
	Murabito, Ettore []
	Swainston, Neil []
	Dada, Joseph O. []
	Khan, Farid []
	Pir, Pinar []
	Simeonidis, Vangelis [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > > ; University of Manchester > Manchester Centre for Integrative Systems Biology]
	Spasic, Irena []
	Wishart, Jill []
	Weichart, Dieter []
	Hayes, Neil W. []
	Jameson, Daniel []
	Broomhead, David S. []
	Oliver, Stephen G. []
	Gaskell, Simon J. []
	McCarthy, John E.G. []
	Paton, Norman W. []
	Westerhoff, Hans V. []
	Kell, Douglas B. []
	Mendes, Pedro []
	* These authors have contributed equally to this work.
Publication date :	2-Sep-2013
Journal title :	FEBS Letters
Publisher :	Elsevier Science
Volume :	587
Issue/season :	17
Pages :	2832-2841
Peer reviewed :	Yes (verified by ORBilu)
Audience :	International
ISSN :	0014-5793
City :	Amsterdam
Country :	The Netherlands
Keywords :	[en] Glycolysis ; Systems biology ; Enzyme kinetics ; Isoenzyme ; Modelling
Abstract :	[en] We present an experimental and computational pipeline for the generation of kinetic models of metabolism, and demonstrate its application to glycolysis in Saccharomyces cerevisiae. Starting from an approximate mathematical model, we employ a ‘‘cycle of knowledge’’ strategy, identifying the steps with most control over flux. Kinetic parameters of the individual isoenzymes within these steps are measured experimentally under a standardised set of conditions. Experimental strategies are applied to establish a set of in vivo concentrations for isoenzymes and metabolites. The data are integrated into a mathematical model that is used to predict a new set of metabolite concentrations and reevaluate the control properties of the system. This bottom-up modelling study reveals that control over the metabolic network most directly involved in yeast glycolysis is more widely distributed than previously thought.
Research centres :	Manchester Centre for Integrative Systems Biology ; Luxembourg Centre for Systems Biomedicine (LCSB): Experimental Neurobiology (Balling Group)
Funders :	BBSRC / EPSRC
Name of the research project :	The Manchester Centre for Integrative Systems Biology
Permalink :	http://hdl.handle.net/10993/3833
DOI :	10.1016/j.febslet.2013.06.043
Other URL :	http://linkinghub.elsevier.com/retrieve/pii/S0014579313005012

	File(s) associated to this reference
	Fulltext file(s): File Commentary Version Size Access Open access Smallbone et al. - 2013 - A model of yeast glycolysis based on a consistent .pdf Publisher postprint 666.13 kB View/Open Additional material(s): File Commentary Size Access Open access MODEL1303260018.xml The final model, as available on the BioModels database. Other version also available form BioModels and from the JWS Online, where they can be simulated online at http://http://jjj.mib.ac.uk/ 214.01 kB View/Open Open access mmc1.pdf The kinetic equations for all enzymes (supplementary data). 89.14 kB View/Open

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