Reference : In situ phenotypic heterogeneity among single cells of the filamentous bacterium Cand...

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology Life sciences : Environmental sciences & ecology Life sciences : Microbiology
	To cite this reference:	http://hdl.handle.net/10993/22293

Title :	In situ phenotypic heterogeneity among single cells of the filamentous bacterium Candidatus Microthrix parvicella
Language :	English
Author, co-author :	Sheik, Abdul [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Muller, Emilie [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Audinot, Jean-Nicolas []
	Lebrun, Laura [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Grysan, Patrick []
	Guignard, Cédric []
	Wilmes, Paul [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Publication date :	2015
Journal title :	ISME Journal (The)
Peer reviewed :	Yes
Audience :	International
ISSN :	1751-7362
e-ISSN :	1751-7370
Abstract :	[en] Microorganisms in biological wastewater treatment plants require adaptive strategies to deal with rapidly fluctuating environmental conditions. At the population level, the filamentous bacterium Candidatus Microthrix parvicella (Ca. M. parvicella) has been found to fine-tune its gene expression for optimized substrate assimilation. Here we investigated in situ substrate assimilation by single cells of Ca. M. parvicella using nano-scale secondary-ion mass spectrometry (nanoSIMS). NanoSIMS imaging highlighted phenotypic heterogeneity among Ca. M. parvicella cells of the same filament, whereby 13C-oleic acid and 13C-glycerol-3-phosphate assimilation occurred in ≈21–55% of cells, despite non-assimilating cells being intact and alive. In response to alternating aerobic–anoxic regimes, 13C-oleic acid assimilation occurred among subpopulations of Ca. M. parvicella cells (≈3–28% of cells). Furthermore, Ca. M. parvicella cells exhibited two temperature optima for 13C-oleic acid assimilation and associated growth rates. These results suggest that phenotypic heterogeneity among Ca. M. parvicella cells allows the population to adapt rapidly to fluctuating environmental conditions facilitating its widespread occurrence in biological wastewater treatment plants.
Research centres :	Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group)
Permalink :	http://hdl.handle.net/10993/22293
DOI :	10.1038/ismej.2015.181

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