Article (Scientific journals)
In situ phenotypic heterogeneity among single cells of the filamentous bacterium Candidatus Microthrix parvicella
SHEIK, Abdul; MULLER, Emilie; Audinot, Jean-Nicolas et al.
2015In ISME Journal
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Abstract :
[en] Microorganisms in biological wastewater treatment plants require adaptive strategies to deal with rapidly fluctuating environmental conditions. At the population level, the filamentous bacterium Candidatus Microthrix parvicella (Ca. M. parvicella) has been found to fine-tune its gene expression for optimized substrate assimilation. Here we investigated in situ substrate assimilation by single cells of Ca. M. parvicella using nano-scale secondary-ion mass spectrometry (nanoSIMS). NanoSIMS imaging highlighted phenotypic heterogeneity among Ca. M. parvicella cells of the same filament, whereby 13C-oleic acid and 13C-glycerol-3-phosphate assimilation occurred in ≈21–55% of cells, despite non-assimilating cells being intact and alive. In response to alternating aerobic–anoxic regimes, 13C-oleic acid assimilation occurred among subpopulations of Ca. M. parvicella cells (≈3–28% of cells). Furthermore, Ca. M. parvicella cells exhibited two temperature optima for 13C-oleic acid assimilation and associated growth rates. These results suggest that phenotypic heterogeneity among Ca. M. parvicella cells allows the population to adapt rapidly to fluctuating environmental conditions facilitating its widespread occurrence in biological wastewater treatment plants.
Research center :
- Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group)
Disciplines :
Environmental sciences & ecology
Biochemistry, biophysics & molecular biology
Microbiology
Author, co-author :
SHEIK, Abdul ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
MULLER, Emilie ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Audinot, Jean-Nicolas
LEBRUN, Laura ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Grysan, Patrick
Guignard, Cédric
WILMES, Paul ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
External co-authors :
no
Language :
English
Title :
In situ phenotypic heterogeneity among single cells of the filamentous bacterium Candidatus Microthrix parvicella
Publication date :
2015
Journal title :
ISME Journal
ISSN :
1751-7370
Publisher :
Nature Publishing Group, United Kingdom
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 29 October 2015

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