Reference : Olfactory neuron-specific expression of A30P alpha-synuclein exacerbates dopamine def...
Scientific journals : Article
Life sciences : Genetics & genetic processes
Olfactory neuron-specific expression of A30P alpha-synuclein exacerbates dopamine deficiency and hyperactivity in a novel conditional model of early Parkinson's disease stages.
Nuber, Silke [> >]
Petrasch-Parwez, Elisabeth [> >]
Arias-Carrion, Oscar [> >]
Koch, Leanie [> >]
Kohl, Zacharias [> >]
Schneider, Jacqueline [> >]
Calaminus, Carsten [> >]
Dermietzel, Rolf [> >]
Samarina, Anna [> >]
Boy, Jana [> >]
Nguyen, Huu P. [> >]
Teismann, Peter [> >]
Velavan, Thirumalaisamy Palanichamy [> >]
Kahle, Philipp J. [> >]
von Horsten, Stephan [> >]
Fendt, Markus [> >]
Krüger, Rejko mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit]
Riess, Olaf [> >]
Neurobiology of Disease
Yes (verified by ORBilu)
United States
[en] Amino Acid Substitution/genetics ; Animals ; Cricetinae ; Disease Models, Animal ; Dopamine/biosynthesis/deficiency ; Female ; Humans ; Hyperkinesis/genetics/metabolism/physiopathology ; Male ; Mice ; Mice, Transgenic ; Mutation/genetics ; Neurons/metabolism/pathology ; Olfactory Bulb/metabolism/pathology/physiopathology ; Parkinsonian Disorders/genetics/metabolism/physiopathology ; alpha-Synuclein/biosynthesis/genetics/physiology
[en] Mutations in the N-terminus of the gene encoding alpha-synuclein (alpha-syn) are linked to autosomal dominantly inherited Parkinson's disease (PD). The vast majority of PD patients develop neuropsychiatric symptoms preceding motor impairments. During this premotor stage, synucleinopathy is first detectable in the olfactory bulb (OB) and brain stem nuclei; however its impact on interconnected brain regions and related symptoms is still less far understood. Using a novel conditional transgenic mouse model, displaying region-specific expression of human mutant alpha-syn, we evaluated effect and reversibility of olfactory synucleinopathy. Our data showed that induction of mutant A30P alpha-syn expression increased transgenic deposition into somatodendritic compartment of dopaminergic neurons, without generating fibrillar inclusions. We found reversibly reduced levels of dopamine and metabolites in the OB, suggesting an impact of A30P alpha-syn on olfactory neurotransmitter content. We further showed that mutant A30P expression led to neurodegenerative changes on an ultrastructural level and a behaviorally hyperactive response correlated with novelty, odor processing and stress associated with an increased dopaminergic tone in midbrain regions. Our present data indicate that mutant (A30P) alpha-syn is directly implicated in reduction of dopamine signaling in OB interneurons, which mediates further alterations in brain regions without transgenic expression leading functionally to a hyperactive response. These modulations of neurotransmission may underlie in part some of the early neuropsychiatric symptoms in PD preceding dysfunction of the nigrostriatal dopaminergic system.
Luxembourg Centre for Systems Biomedicine (LCSB): Clinical & Experimental Neuroscience (Krüger Group)
Copyright (c) 2011 Elsevier Inc. All rights reserved.

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