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Functional relevance of ceruloplasmin mutations in Parkinson's disease.
Hochstrasser, Helmine; Tomiuk, Jurgen; Walter, Uwe et al.
2005In FASEB Journal, 19 (13), p. 1851-3
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Keywords :
Alleles; Cell Line; Ceruloplasmin/biosynthesis/chemistry/genetics/metabolism; Endoplasmic Reticulum/metabolism; Epithelial Cells/cytology; Ferritins/chemistry; Fluorescent Antibody Technique, Indirect; Glycosylation; Glycosylphosphatidylinositols/chemistry; Heterozygote; Humans; Immunoprecipitation; Iron/chemistry/metabolism; Kidney/pathology; Microscopy, Fluorescence; Mutation; Mutation, Missense; Neurodegenerative Diseases/pathology; Oxidative Stress; Parkinson Disease/genetics/pathology; Protein Denaturation; Protein Folding; Protein Isoforms; Substantia Nigra/metabolism/pathology; Transfection; Transferrin/chemistry
Abstract :
[en] Increased iron levels of the substantia nigra and the discovery of ceruloplasmin mutations in patients with Parkinson's disease (PD) imply impaired iron metabolism in this neurodegenerative disorder. Ceruloplasmin has ferroxidase activity oxidizing iron(II) to iron(III). In the present study, we analyzed the amount of ceruloplasmin, iron, ferritin, and transferrin and the ceruloplasmin ferroxidase activity in serum of patients with the diagnosis of PD carrying the ceruloplasmin mutations I63T, D544E, and R793H. The impact of these missense mutations on the biosynthesis of holo-ceruloplasmin was investigated in cell culture experiments. Functional relevance was found for the ceruloplasmin mutations I63T and D544E. In vivo, the I63T mutation resulted in half the normal ceruloplasmin concentration and markedly reduced ferroxidase activity in serum from a heteroallelic PD patient. In cell culture, the I63T glycosylphosphatidylinositol (GPI)-linked ceruloplasmin isoform was retained in the endoplasmatic reticulum of human embryonic kidney cells. Furthermore, the D544E polymorphism resulted in significantly reduced serum ceruloplasmin levels and ferroxidase activity in heteroallelic patients and in expression of mainly apo-ceruloplasmin in cell culture. Our studies indicate that altered activity of ceruloplasmin may present a vulnerability factor for iron induced oxidative stress in PD.
Research center :
- Luxembourg Centre for Systems Biomedicine (LCSB): Clinical & Experimental Neuroscience (Krüger Group)
Disciplines :
Genetics & genetic processes
Author, co-author :
Hochstrasser, Helmine
Tomiuk, Jurgen
Walter, Uwe
Behnke, Stefanie
Spiegel, Jorg
Krüger, Rejko ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Becker, Georg
Riess, Olaf
Berg, Daniela
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Title :
Functional relevance of ceruloplasmin mutations in Parkinson's disease.
Publication date :
Journal title :
FASEB Journal
Publisher :
Federation of American Societies for Experimental Biology, United States - Maryland
Volume :
Issue :
Pages :
Peer reviewed :
Peer Reviewed verified by ORBi
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