Article (Scientific journals)
The histone deacetylase inhibitor trichostatin A mediates upregulation of 5-lipoxygenase promoter activity by recruitment of Sp1 to distinct GC-boxes
Schnur, N.; SEUTER, Sabine; Katryniok, C. et al.
2007In Biochimica et Biophysica Acta, 1771 (10), p. 1271-1282
Peer reviewed
 

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Keywords :
RNA, Messenger; genetics; pharmacology; antagonists & inhibitors; metabolism; Arachidonate 5-Lipoxygenase; Base Sequence; Enzyme Inhibitors; Gene Expression Regulation, Enzymologic; Hela Cells; Histone Deacetylases; Humans; Hydroxamic Acids; Molecular Sequence Data; Promoter Regions (Genetics); Protein Synthesis Inhibitors; RNA Polymerase II; Sp1 Transcription Factor
Abstract :
[en] The histone deacetylase inhibitor trichostatin A (TsA) potently induces 5-lipoxygenase (5-LO) promoter activity in reporter gene assays as well as 5-LO mRNA expression. We identified two proximal Sp1/Sp3 binding sites in the 5-LO gene promoter mediating the TsA effect in both 5-LO-negative HeLa cells and in 5-LO expressing Mono Mac 6 (MM6) cells, the tandem GC-boxes, by contrast, were not important for the TsA effect. TsA neither altered the protein expression levels of Sp1/Sp3 nor of the histone deacetylases HDAC1/2, nor did it apparently change the protein complex formation by these factors. Also, treatment of cells with TsA did not change the binding affinity of Sp1/Sp3 in cell extracts, as tested by DAPA analysis using probes containing the proximal GC boxes. However, in the living cell TsA induced Sp1, Sp3 and RNA polymerase II recruitment to the 5-LO promoter without changing the acetylation status of histone protein H4. Cotransfection studies suggest that both Sp1 and Sp3 can mediate the TsA effect. This is the first report demonstrating that Sp3 is involved in the regulation of 5-LO promoter activity. In summary, we show that TsA increases 5-LO promoter activity by the enhanced recruitment of Sp1 and Sp3 to the 5-LO promoter.
Disciplines :
Life sciences: Multidisciplinary, general & others
Identifiers :
UNILU:UL-ARTICLE-2008-688
Author, co-author :
Schnur, N.
SEUTER, Sabine ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Katryniok, C.
Rådmark, O.
Steinhilber, D.
Language :
English
Title :
The histone deacetylase inhibitor trichostatin A mediates upregulation of 5-lipoxygenase promoter activity by recruitment of Sp1 to distinct GC-boxes
Publication date :
October 2007
Journal title :
Biochimica et Biophysica Acta
ISSN :
0006-3002
Volume :
1771
Issue :
10
Pages :
1271-1282
Peer reviewed :
Peer reviewed
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since 14 March 2014

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