Reference : Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing sig...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor
Kischkel, F. C. [> >]
Hellbardt, S. [> >]
Behrmann, Iris mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Germer, M. [> >]
Pawlita, M. [> >]
Krammer, P. H. [> >]
Peter, M. E. [> >]
EMBO Journal
Oxford University Press
Yes (verified by ORBilu)
United Kingdom
[en] Adaptor Proteins, Signal Transducing ; Phosphorylation ; Molecular Sequence Data ; Lymphocytes ; Humans ; Fas-Associated Death Domain Protein ; DNA Primers ; Cysteine Endopeptidases ; Cross-Linking Reagents ; Cell Line ; Caspases ; Caspase 9 ; Caspase 8 ; Carrier Proteins ; Base Sequence ; Apoptosis ; Antigens, CD95 ; Animals ; Tumor Cells, Cultured
[en] APO-1 (Fas/CD95), a member of the tumor necrosis factor receptor superfamily, induces apoptosis upon receptor oligomerization. In a search to identify intracellular signaling molecules coupling to oligomerized APO-1, several cytotoxicity-dependent APO-1-associated proteins (CAP) were immunoprecipitated from the apoptosis-sensitive human leukemic T cell line HUT78 and the lymphoblastoid B cell line SKW6.4. CAP1-3 (27-29 kDa) and CAP4 (55 kDa), instantly detectable after the crosslinking of APO-1, were associated only with aggregated (the signaling form of APO-1) and not with monomeric APO-1. CAP1 and CAP2 were identified as serine phosphorylated MORT1/FADD. The association of CAP1-4 with APO-1 was not observed with C-terminally truncated non-signaling APO-1. In addition, CAP1 and CAP2 did not associate with an APO-1 cytoplasmic tail carrying the lprcg amino acid replacement. Moreover, no APO-1-CAP association was found in the APO-1+, anti-APO-1-resistant pre-B cell line Boe. Our data suggest that in vivo CAP1-4 are the APO-1 apoptosis-transducing molecules.

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