Reference : Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing sig... |
Scientific journals : Article | |||
Life sciences : Biochemistry, biophysics & molecular biology | |||
http://hdl.handle.net/10993/6263 | |||
Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor | |
English | |
Kischkel, F. C. [> >] | |
Hellbardt, S. [> >] | |
Behrmann, Iris ![]() | |
Germer, M. [> >] | |
Pawlita, M. [> >] | |
Krammer, P. H. [> >] | |
Peter, M. E. [> >] | |
1996 | |
EMBO Journal | |
Oxford University Press | |
14 | |
22 | |
5579-88 | |
Yes (verified by ORBilu) | |
0261-4189 | |
1460-2075 | |
Oxford | |
United Kingdom | |
[en] Adaptor Proteins, Signal Transducing ; Phosphorylation ; Molecular Sequence Data ; Lymphocytes ; Humans ; Fas-Associated Death Domain Protein ; DNA Primers ; Cysteine Endopeptidases ; Cross-Linking Reagents ; Cell Line ; Caspases ; Caspase 9 ; Caspase 8 ; Carrier Proteins ; Base Sequence ; Apoptosis ; Antigens, CD95 ; Animals ; Tumor Cells, Cultured | |
[en] APO-1 (Fas/CD95), a member of the tumor necrosis factor receptor superfamily, induces apoptosis upon receptor oligomerization. In a search to identify intracellular signaling molecules coupling to oligomerized APO-1, several cytotoxicity-dependent APO-1-associated proteins (CAP) were immunoprecipitated from the apoptosis-sensitive human leukemic T cell line HUT78 and the lymphoblastoid B cell line SKW6.4. CAP1-3 (27-29 kDa) and CAP4 (55 kDa), instantly detectable after the crosslinking of APO-1, were associated only with aggregated (the signaling form of APO-1) and not with monomeric APO-1. CAP1 and CAP2 were identified as serine phosphorylated MORT1/FADD. The association of CAP1-4 with APO-1 was not observed with C-terminally truncated non-signaling APO-1. In addition, CAP1 and CAP2 did not associate with an APO-1 cytoplasmic tail carrying the lprcg amino acid replacement. Moreover, no APO-1-CAP association was found in the APO-1+, anti-APO-1-resistant pre-B cell line Boe. Our data suggest that in vivo CAP1-4 are the APO-1 apoptosis-transducing molecules. | |
http://hdl.handle.net/10993/6263 |
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