| Reference : Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing sig... |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/10993/6263 | |||
| Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor | |
| English | |
| Kischkel, F. C. [> >] | |
| Hellbardt, S. [> >] | |
Behrmann, Iris  [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >] | |
| Germer, M. [> >] | |
| Pawlita, M. [> >] | |
| Krammer, P. H. [> >] | |
| Peter, M. E. [> >] | |
| 1996 | |
| EMBO Journal | |
| Oxford University Press | |
| 14 | |
| 22 | |
| 5579-88 | |
| Yes (verified by ORBilu) | |
| 0261-4189 | |
| 1460-2075 | |
| Oxford | |
| United Kingdom | |
| [en] Adaptor Proteins, Signal Transducing ; Phosphorylation ; Molecular Sequence Data ; Lymphocytes ; Humans ; Fas-Associated Death Domain Protein ; DNA Primers ; Cysteine Endopeptidases ; Cross-Linking Reagents ; Cell Line ; Caspases ; Caspase 9 ; Caspase 8 ; Carrier Proteins ; Base Sequence ; Apoptosis ; Antigens, CD95 ; Animals ; Tumor Cells, Cultured | |
| [en] APO-1 (Fas/CD95), a member of the tumor necrosis factor receptor superfamily, induces apoptosis upon receptor oligomerization. In a search to identify intracellular signaling molecules coupling to oligomerized APO-1, several cytotoxicity-dependent APO-1-associated proteins (CAP) were immunoprecipitated from the apoptosis-sensitive human leukemic T cell line HUT78 and the lymphoblastoid B cell line SKW6.4. CAP1-3 (27-29 kDa) and CAP4 (55 kDa), instantly detectable after the crosslinking of APO-1, were associated only with aggregated (the signaling form of APO-1) and not with monomeric APO-1. CAP1 and CAP2 were identified as serine phosphorylated MORT1/FADD. The association of CAP1-4 with APO-1 was not observed with C-terminally truncated non-signaling APO-1. In addition, CAP1 and CAP2 did not associate with an APO-1 cytoplasmic tail carrying the lprcg amino acid replacement. Moreover, no APO-1-CAP association was found in the APO-1+, anti-APO-1-resistant pre-B cell line Boe. Our data suggest that in vivo CAP1-4 are the APO-1 apoptosis-transducing molecules. | |
| http://hdl.handle.net/10993/6263 |
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