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12 September 2023
Article (Scientific journals)
Neurons undergo pathogenic metabolic reprogramming in models of familial ALS.
Riechers, Sean-Patrick Hermann; Mojsilovic-Petrovic, Jelena; Belton, Tayler B. et al.
2022In Molecular Metabolism, 60, p. 101468
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Keywords :
Adenosine Triphosphate; Amyotrophic Lateral Sclerosis/genetics/metabolism/pathology; Humans; Neurodegenerative Diseases/pathology; Neurons/metabolism; Superoxide Dismutase/genetics/metabolism; ATP; G6PDH; Glycolysis; Neuron; Redox
Abstract :
[en] OBJECTIVES: Normal cellular function requires a rate of ATP production sufficient to meet demand. In most neurodegenerative diseases (including Amyotrophic Lateral Sclerosis [ALS]), mitochondrial dysfunction is postulated raising the possibility of impaired ATP production and a need for compensatory maneuvers to sustain the ATP production/demand balance. We investigated intermediary metabolism of neurons expressing familial ALS (fALS) genes and interrogated the functional consequences of glycolysis genes in fitness assays and neuronal survival. METHODS: We created a pure neuronal model system for isotopologue investigations of fuel utilization. In a yeast platform we studied the functional contributions of glycolysis genes in a growth fitness assay iafter expressing of a fALS gene. RESULTS: We find in our rodent models of fALS, a reduction in neuronal lactate production with maintained or enhanced activity of the neuronal citric acid cycle. This rewiring of metabolism is associated with normal ATP levels, bioenergetics, and redox status, thus supporting the notion that gross mitochondrial function is not compromised in neurons soon after expressing fALS genes. Genetic loss-of-function manipulation of individual steps in the glycolysis and the pentose phosphate pathway blunt the negative phenotypes seen in various fALS models. CONCLUSIONS: We propose that neurons adjust fuel utilization in the setting of neurodegenerative disease-associated alteration in mitochondrial function in a baleful manner and targeting this process can be healthful.
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Author, co-author :
Riechers, Sean-Patrick Hermann ;  Department of Neurology, Feinberg School of Medicine, Northwestern University, United States.
Mojsilovic-Petrovic, Jelena
Belton, Tayler B.
Chakrabarty, Ram P.
Garjani, Mehraveh
Medvedeva, Valentina
Dalton, Casey
Wong, Yvette C.
Chandel, Navdeep S.
Dienel, Gerald
Kalb, Robert G.
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Language :
Title :
Neurons undergo pathogenic metabolic reprogramming in models of familial ALS.
Publication date :
Journal title :
Molecular Metabolism
Publisher :
Elsevier, Berlin, Germany
Volume :
Pages :
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Published by Elsevier GmbH.


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