Article (Scientific journals)
Expression and function of resolvin RvD1(n-3 DPA) receptors in oral epithelial cells
Balta, Maria G.; Schreurs, Olav; Hansen, Trond V. et al.
2022In European Journal of Oral Sciences, 130 (4), p. 12883
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The original article is available at: https://doi.org/10.1111%2Feos.12883


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Keywords :
Calcium; Docosahexaenoic Acids/pharmacology; Epithelial Cells/metabolism; Humans; Inflammation/pathology; Receptors, Formyl Peptide/genetics/metabolism; beta-Defensins; DRV1/GPR32; FPR2/ALX; beta defensin; cathelicidin; oral epithelium
Abstract :
[en] Chronic inflammatory responses can inflict permanent damage to host tissues. Specialized pro-resolving mediators downregulate inflammation but also can have other functions. The aim of this study was to examine whether oral epithelial cells express the receptors FPR2/ALX and DRV1/GPR32, which bind RvD1(n-3 DPA) , a recently described pro-resolving mediator derived from omega-3 docosapentaenoic acid (DPA), and whether RvD1(n-3 DPA) exposure induced significant responses in these cells. Gingival biopsies were stained using antibodies to FPR2/ALX and DRV1/GPR32. Expression of FPR2/ALX and DRV1/GPR32 was examined in primary oral epithelial cells by qRT-PCR, flow cytometry, and immunofluorescence. The effect of RvD1(n-3 DPA) on intracellular calcium mobilization and transcription of beta-defensins 1 and 2, and cathelicidin was evaluated by qRT-PCR. FPR2/ALX and DRV1/GPR32 were expressed by gingival keratinocytes in situ. In cultured oral epithelial cells, FPR2/ALX was detected on the cell surface, whereas FPR2/ALX and DRV1/GPR32 were detected intracellularly. Exposure to RvD1(n-3 DPA) induced intracellular calcium mobilization, FPR2/ALX internalization, DRV1/GPR32 translocation to the nucleus, and significantly increased expression of genes coding for beta-defensin 1, beta-defensin 2, and cathelicidin. This shows that the signal constituted by RvD1(n-3 DPA) is recognized by oral keratinocytes and that this can strengthen the antimicrobial and regulatory potential of the oral epithelium.
Research center :
- Luxembourg Centre for Systems Biomedicine (LCSB): Biomedical Data Science (Glaab Group)
Disciplines :
Human health sciences: Multidisciplinary, general & others
Life sciences: Multidisciplinary, general & others
Author, co-author :
Balta, Maria G.
Schreurs, Olav
Hansen, Trond V.
Tungen, Jørn E.
Vik, Anders
Glaab, Enrico  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Biomedical Data Science
Küntziger, Thomas M.
Schenck, Karl
Baekkevold, Espen S.
Blix, Inger Johanne S.
External co-authors :
yes
Language :
English
Title :
Expression and function of resolvin RvD1(n-3 DPA) receptors in oral epithelial cells
Publication date :
2022
Journal title :
European Journal of Oral Sciences
ISSN :
1600-0722
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
130
Issue :
4
Pages :
e12883
Peer reviewed :
Peer Reviewed verified by ORBi
Focus Area :
Systems Biomedicine
Available on ORBilu :
since 01 January 2023

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