Reference : Expression and function of resolvin RvD1(n-3 DPA) receptors in oral epithelial cells
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
Human health sciences : Multidisciplinary, general & others
Systems Biomedicine
Expression and function of resolvin RvD1(n-3 DPA) receptors in oral epithelial cells
Balta, Maria G. [> >]
Schreurs, Olav [> >]
Hansen, Trond V. [> >]
Tungen, Jørn E. [> >]
Vik, Anders [> >]
Glaab, Enrico mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Biomedical Data Science]
Küntziger, Thomas M. [> >]
Schenck, Karl [> >]
Baekkevold, Espen S. [> >]
Blix, Inger Johanne S. [> >]
European journal of oral sciences
[en] Calcium ; Docosahexaenoic Acids/pharmacology ; Epithelial Cells/metabolism ; Humans ; Inflammation/pathology ; Receptors, Formyl Peptide/genetics/metabolism ; beta-Defensins ; DRV1/GPR32 ; FPR2/ALX ; beta defensin ; cathelicidin ; oral epithelium
[en] Chronic inflammatory responses can inflict permanent damage to host tissues. Specialized pro-resolving mediators downregulate inflammation but also can have other functions. The aim of this study was to examine whether oral epithelial cells express the receptors FPR2/ALX and DRV1/GPR32, which bind RvD1(n-3 DPA) , a recently described pro-resolving mediator derived from omega-3 docosapentaenoic acid (DPA), and whether RvD1(n-3 DPA) exposure induced significant responses in these cells. Gingival biopsies were stained using antibodies to FPR2/ALX and DRV1/GPR32. Expression of FPR2/ALX and DRV1/GPR32 was examined in primary oral epithelial cells by qRT-PCR, flow cytometry, and immunofluorescence. The effect of RvD1(n-3 DPA) on intracellular calcium mobilization and transcription of beta-defensins 1 and 2, and cathelicidin was evaluated by qRT-PCR. FPR2/ALX and DRV1/GPR32 were expressed by gingival keratinocytes in situ. In cultured oral epithelial cells, FPR2/ALX was detected on the cell surface, whereas FPR2/ALX and DRV1/GPR32 were detected intracellularly. Exposure to RvD1(n-3 DPA) induced intracellular calcium mobilization, FPR2/ALX internalization, DRV1/GPR32 translocation to the nucleus, and significantly increased expression of genes coding for beta-defensin 1, beta-defensin 2, and cathelicidin. This shows that the signal constituted by RvD1(n-3 DPA) is recognized by oral keratinocytes and that this can strengthen the antimicrobial and regulatory potential of the oral epithelium.
Luxembourg Centre for Systems Biomedicine (LCSB): Biomedical Data Science (Glaab Group)
Researchers ; Professionals ; Students
The original article is available at:

File(s) associated to this reference

Fulltext file(s):

Open access
EOS-130-0.pdfPublisher postprint1.71 MBView/Open

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.