Article (Scientific journals)
The Evolution of Erythrocytes Becoming Red in Respect to Fluorescence
Hertz, Laura; Ruppenthal, Sandra; Simionato, Greta et al.
2019In Frontiers in Physiology, 10
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Keywords :
mouse model; transfusion; fluorescent protein; intravital microscopy imaging MONOMERIC RED; PROTEIN; C57BL/6; CALCIUM Physiology Physiology lars\_kaestner@me.com Wagner; Christian/A-1307-2009 Wagner; Christian/0000-0001-7788-4594 European Seventh Framework ProgramEuropean Union (EU) [602121] Deutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG) [Sonderforschungsbereich SFB894]; European Framework ``Horizon 2020'' [675115] This study was supported by the European Seventh Framework Program under grant agreement number 602121 (CoMMiTMenT; LK); the European Framework ``Horizon 2020'' under grant agreement number 675115 (RELEVANCE; CW and LK); and the Deutsche Forschungsgemeinschaft (DFG) Sonderforschungsbereich SFB894 (PW and UB). 32 0 1 1 Front. Physiol. IE0BO ISI:000472051700001
Abstract :
[en] Very young red blood cells, namely reticulocytes, can be quite easily recognized and labeled by cluster of differentiation antibodies (CD71 transferrin receptor) or by staining remnant RNA with thiazol orange. In contrast, age specific erythrocyte labeling is more difficult in later periods of their life time. While erythrocytes contain band 4.1 protein a molecular clock, so far it has not been possible to read this clock on individual cells. One concept to track erythrocytes during their life time is to mark them when they are young, either directly in vivo or ex vivo followed by a transfusion. Several methods like biotinylation, use of isotopes or fluorescent labeling have proved to be useful experimental approaches but also have several inherent disadvantages. Genetic engineering of mice provides additional options to express fluorescent proteins in erythrocytes. To allow co-staining with popular green fluorescent dyes like Fluo-4 or other fluorescein-based dyes, we bred a mouse line expressing a tandem red fluorescent protein (tdRFP). Within this Brief Research Report, we provide the initial characterisation of this mouse line and show application examples ranging from transfusion experiments and intravital microscopy to multicolour flow cytometry and confocal imaging. We provide a versatile new tool for erythrocyte research and discuss a range of experimental opportunities to study membrane processes and other aspects of erythrocyte development and aging with help of these animals.
Disciplines :
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Hertz, Laura;  Kaestner, L (Reprint Author), Saarland Univ, Theoret Med Biosci, Homburg, Germany. Kaestner, L (Reprint Author), Saarland Univ, Expt Phys, Saarbrucken, Germany. Hertz, Laura
Ruppenthal, Sandra;  Ruppenthal, Sandra
Simionato, Greta;  Petkoya-Kirova, Polina, Saarland Univ, Inst Mol Cell Biol, Homburg, Germany. Simionato, Greta
Stephan, Quint;  Kaestner, Lars, Saarland Univ, Theoret Med Biosci, Homburg, Germany. Simionato, Greta
Kihm, Alexander;  Quint, Stephan
Abay, Asena;  Kihm, Alexander
Petkoya-Kirova, Polina;  Abay, Asena
Boehm, Ulrich;  Wagner, Christian
Weissgerber, Petra;  Kaestner, Lars, Saarland Univ, Expt Phys, Saarbrucken, Germany. Boehm, Ulrich
WAGNER, Christian ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit
Laschke, Matthias W.
Kaestner, Lars
External co-authors :
yes
Title :
The Evolution of Erythrocytes Becoming Red in Respect to Fluorescence
Publication date :
2019
Journal title :
Frontiers in Physiology
ISSN :
1664-042X
Publisher :
FRONTIERS MEDIA SA, AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND, Unknown/unspecified
Volume :
10
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Article
Available on ORBilu :
since 15 January 2020

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