Article (Scientific journals)
Functional and phenotypic differences of pure populations of stem cell-derived astrocytes and neuronal precursor cells
Kleiderman, Susanne; Sá, Joao; Teixeira, Ana et al.
2016In Glia, 64 (5), p. 695-715
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This is the peer reviewed version of the following article: Kleiderman et al., Glia, 2015, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/glia.22954/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.


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Keywords :
astrocytes; neural stem cells; differentiation; metabolic flux; transcriptome; microarray; bioinformatics; statistics
Abstract :
[en] Availability of homogeneous astrocyte populations would facilitate research concerning cell plasticity (metabolic and transcriptional adaptations; innate immune responses) and cell cycle reactivation. Current protocols to prepare astrocyte cultures differ in their final content of immature precursor cells, pre-activated cells or entirely different cell types. A new method taking care of all these issues would improve research on astrocyte functions. We found here that the exposure of a defined population of pluripotent stem cell-derived neural stem cells (NSC) to BMP4 results in pure, non-proliferating astrocyte cultures within 24-48 h. These murine astrocytes generated from embryonic stem cells (mAGES) expressed the positive markers GFAP, aquaporin 4 and GLT-1, supported neuronal function, and acquired innate immune functions such as the response to TNF and IL-1. The protocol was applicable to several normal or disease-prone pluripotent cell lines, and the corresponding mAGES all exited the cell cycle and lost most of their nestin expression, in contrast to astrocytes generated by serum-addition or obtained as primary cultures. Comparative gene expression analysis of mAGES and NSC allowed quantification of differences between the two cell types and a definition of an improved maker set to define astrocytes. Inclusion of several published data sets in this transcriptome comparison revealed the similarity of mAGES with cortical astrocytes in vivo. Metabolic analysis of homogeneous NSC and astrocyte populations revealed distinct neurochemical features: both cell types synthesized glutamine and citrate, but only mature astrocytes released these metabolites. Thus, the homogeneous cultures allowed an improved definition of NSC and astrocyte features.
Research center :
- Luxembourg Centre for Systems Biomedicine (LCSB): Biomedical Data Science (Glaab Group)
ULHPC - University of Luxembourg: High Performance Computing
Disciplines :
Biotechnology
Neurology
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Kleiderman, Susanne
Sá, Joao
Teixeira, Ana
Brito, Catarina
Gutbier, Simon
Evje, Lars
Hadera, Mussie
Glaab, Enrico  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Henry, Margit
Agapios, Sachinidis
Alves, Paula
Sonnewald, Ursula
Leist, Marcel
More authors (3 more) Less
External co-authors :
yes
Language :
English
Title :
Functional and phenotypic differences of pure populations of stem cell-derived astrocytes and neuronal precursor cells
Publication date :
2016
Journal title :
Glia
ISSN :
1098-1136
Publisher :
Wiley Liss, Inc., New York, United States - New York
Volume :
64
Issue :
5
Pages :
695-715
Peer reviewed :
Peer Reviewed verified by ORBi
FnR Project :
FNR5782168 - Exploring Parkinson'S Disease Inhibitor Efficacy On A Non-dopaminergic Target, 2013 (01/12/2013-31/05/2016) - Enrico Glaab
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since 23 November 2015

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