Reference : Plasma membrane Ca2+ ATPase 4 is required for sperm motility and male fertility.
Scientific journals : Article
Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics)
Plasma membrane Ca2+ ATPase 4 is required for sperm motility and male fertility.
Schuh, Kai [> >]
Cartwright, Elizabeth J. [> >]
Jankevics, Eriks [> >]
Bundschu, Karin [> >]
Liebermann, Jurgen [> >]
Williams, Judith C. [> >]
Armesilla, Angel L. [> >]
Emerson, Michael [> >]
Oceandy, Delvac [> >]
Knobeloch, Klaus-Peter [> >]
Neyses, Ludwig mailto [University of Luxembourg > Research Office]
The Journal of biological chemistry
Yes (verified by ORBilu)
United States
[en] Alternative Splicing ; Animals ; Blotting, Northern ; Blotting, Southern ; Blotting, Western ; Calcium/metabolism ; Calcium-Transporting ATPases/biosynthesis ; Cation Transport Proteins ; Cloning, Molecular ; DNA, Complementary/metabolism ; Fertility ; Fertilization in Vitro ; Fluoresceins/pharmacology ; Fluorescent Dyes/pharmacology ; Genotype ; Humans ; Male ; Mice ; Mice, Knockout ; Microscopy, Fluorescence ; Models, Genetic ; Molecular Sequence Data ; Plasma Membrane Calcium-Transporting ATPases ; Protein Isoforms ; Protein Structure, Tertiary ; Rats ; Recombination, Genetic ; Reverse Transcriptase Polymerase Chain Reaction ; Sperm Motility ; Succinimides/pharmacology ; Testis/metabolism ; Time Factors
[en] Calcium and Ca(2+)-dependent signals play a crucial role in sperm motility and mammalian fertilization, but the molecules and mechanisms underlying these Ca(2+)-dependent pathways are incompletely understood. Here we show that homozygous male mice with a targeted gene deletion of isoform 4 of the plasma membrane calcium/calmodulin-dependent calcium ATPase (PMCA), which is highly enriched in the sperm tail, are infertile due to severely impaired sperm motility. Furthermore, the PMCA inhibitor 5-(and-6)-carboxyeosin diacetate succinimidyl ester reduced sperm motility in wild-type animals, thus mimicking the effects of PMCA4 deficiency on sperm motility and supporting the hypothesis of a pivotal role of the PMCA4 on the regulation of sperm function and intracellular Ca(2+) levels.

File(s) associated to this reference

Fulltext file(s):

Limited access
Plasma membrane Ca2+ ATPase 4 is required for sperm motility and male fertility.pdfPublisher postprint916.41 kBRequest a copy

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.