Article (Scientific journals)
Disruption of the interaction between PMCA2 and calcineurin triggers apoptosis and enhances paclitaxel-induced cytotoxicity in breast cancer cells.
Baggott, Rhiannon R.; Mohamed, Tamer M. A.; Oceandy, Delvac et al.
2012In Carcinogenesis, 33 (12), p. 2362-8
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Keywords :
Antineoplastic Agents, Phytogenic/pharmacology; Apoptosis/drug effects; Breast Neoplasms/drug therapy/pathology; Calcineurin/physiology; Cell Line, Tumor; Fas Ligand Protein/analysis; Genes, p53; Humans; NFATC Transcription Factors/physiology; Paclitaxel/pharmacology; Plasma Membrane Calcium-Transporting ATPases/physiology; Protein Binding/drug effects; Signal Transduction
Abstract :
[en] Cancer is caused by defects in the signalling mechanisms that govern cell proliferation and apoptosis. It is well known that calcium-dependent signalling pathways play a critical role in cell regulation. A tight control of calcium homeostasis by transporters and channel proteins is required to assure a proper functioning of the calcium-sensitive signal transduction pathways that regulate cell growth and apoptosis. The plasma membrane calcium ATPase 2 (PMCA2) has been recently identified as a negative regulator of apoptosis that can play a significant role in cancer progression by conferring cells resistance to apoptosis. We have previously reported an inhibitory interaction between PMCA2 and the calcium-activated signalling molecule calcineurin in breast cancer cells. Here, we demonstrate that disruption of the PMCA2/calcineurin interaction in a variety of human breast cancer cells results in activation of the calcineurin/NFAT pathway, upregulation in the expression of the pro-apoptotic protein Fas Ligand and in a concomitant loss of cell viability. Reduction in cell viability is the consequence of an increase in cell apoptosis. Impairment of the PMCA2/calcineurin interaction enhances paclitaxel-mediated cytotoxicity of breast tumoral cells. Our results suggest that therapeutic modulation of the PMCA2/calcineurin interaction might have important clinical applications to improve current treatments for breast cancer patients.
Disciplines :
Oncology
Author, co-author :
Baggott, Rhiannon R.
Mohamed, Tamer M. A.
Oceandy, Delvac
Holton, Marylouisa
Blanc, Marie Cecile
Roux-Soro, Sandrine C.
Brown, Sarah
Brown, James E.
Cartwright, Elizabeth J.
Wang, Weiguang
NEYSES, Ludwig ;  University of Luxembourg > Research Office
Armesilla, Angel L.
Language :
English
Title :
Disruption of the interaction between PMCA2 and calcineurin triggers apoptosis and enhances paclitaxel-induced cytotoxicity in breast cancer cells.
Publication date :
2012
Journal title :
Carcinogenesis
ISSN :
0143-3334
eISSN :
1460-2180
Publisher :
Oxford University Press, United Kingdom
Volume :
33
Issue :
12
Pages :
2362-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 16 October 2014

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