Article (Scientific journals)
Expression of the plasma membrane Ca2+-ATPase in myogenic cells.
Hammes, A.; Oberdorf-Maass, S.; Jenatschke, S. et al.
1996In The Journal of biological chemistry, 271 (48), p. 30816-22
Peer reviewed


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Keywords :
Animals; Calcium/metabolism; Calcium-Transporting ATPases/metabolism; Cell Differentiation; Cell Membrane/enzymology; Cells, Cultured; Creatine Kinase/metabolism; Humans; Muscles/enzymology; RNA, Messenger/genetics; Rats; Recombinant Proteins
Abstract :
[en] To study the physiological function of the plasma membrane calmodulin-dependent calcium ATPase (PMCA) in intact cells, L6 myogenic cell lines stably overexpressing the human PMCA isoform 4CI (= human PMCA isoform 4b) were generated. Several independent L6 clones and controls stably transfected with the empty expression vector were analyzed in detail. The resting cytosolic calcium level in hPMCA4CI-overexpressing muscle cells (measured by the Fura-2 method) was significantly reduced by 20-30% compared with controls. This was shown in a cytosolic window of 1322 single cells (p < 0.01). Furthermore, the differentiation process of these cells was remarkably accelerated compared with control myoblasts and parental nontransfected L6 cells as assessed by multinucleated myotube formation and creatine phosphokinase activity elevation. After 4 and 6 days of differentiation, PMCA-overexpressing L6 cells from four independent clones displayed a 3- and 4-fold higher creatine phosphokinase activity compared with controls (n = 5, p < 0.02). These results may extend the concept of the function of the PMCA from simple prevention of calcium overload to an active involvement in intracellular calcium regulation with potentially important consequences for cellular functions.
Disciplines :
Cardiovascular & respiratory systems
Author, co-author :
Hammes, A.
Oberdorf-Maass, S.
Jenatschke, S.
Pelzer, T.
Maass, A.
Gollnick, F.
Meyer, R.
Afflerbach, J.
Neyses, Ludwig ;  University of Luxembourg > Reserach Office
Language :
Title :
Expression of the plasma membrane Ca2+-ATPase in myogenic cells.
Publication date :
Journal title :
The Journal of biological chemistry
Volume :
Issue :
Pages :
Peer reviewed :
Peer reviewed
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