Article (Scientific journals)
Basic Regulatory Principles of Escherichia coli's Electron Transport Chain for Varying Oxygen Conditions.
Henkel, Sebastian G.; Beek, Alexander Ter; Steinsiek, Sonja et al.
2014In PLoS ONE, 9 (9), p. 107640
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Keywords :
systems biology; E. coli
Abstract :
[en] For adaptation between anaerobic, micro-aerobic and aerobic conditions Escherichia coli's metabolism and in particular its electron transport chain (ETC) is highly regulated. Although it is known that the global transcriptional regulators FNR and ArcA are involved in oxygen response it is unclear how they interplay in the regulation of ETC enzymes under micro-aerobic chemostat conditions. Also, there are diverse results which and how quinones (oxidised/reduced, ubiquinone/other quinones) are controlling the ArcBA two-component system. In the following a mathematical model of the E. coli ETC linked to basic modules for substrate uptake, fermentation product excretion and biomass formation is introduced. The kinetic modelling focusses on regulatory principles of the ETC for varying oxygen conditions in glucose-limited continuous cultures. The model is based on the balance of electron donation (glucose) and acceptance (oxygen or other acceptors). Also, it is able to account for different chemostat conditions due to changed substrate concentrations and dilution rates. The parameter identification process is divided into an estimation and a validation step based on previously published and new experimental data. The model shows that experimentally observed, qualitatively different behaviour of the ubiquinone redox state and the ArcA activity profile in the micro-aerobic range for different experimental conditions can emerge from a single network structure. The network structure features a strong feed-forward effect from the FNR regulatory system to the ArcBA regulatory system via a common control of the dehydrogenases of the ETC. The model supports the hypothesis that ubiquinone but not ubiquinol plays a key role in determining the activity of ArcBA in a glucose-limited chemostat at micro-aerobic conditions.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Henkel, Sebastian G.
Beek, Alexander Ter
Steinsiek, Sonja
Stagge, Stefan
Bettenbrock, Katja
de Mattos, M. Joost Teixeira
SAUTER, Thomas ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Sawodny, Oliver
Ederer, Michael
Language :
English
Title :
Basic Regulatory Principles of Escherichia coli's Electron Transport Chain for Varying Oxygen Conditions.
Publication date :
2014
Journal title :
PLoS ONE
ISSN :
1932-6203
Publisher :
Public Library of Science, United States - California
Volume :
9
Issue :
9
Pages :
e107640
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 02 October 2014

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