Article (Scientific journals)
ILQINS hexapeptide, identified in lysozyme left-handed helical ribbons and nanotubes, forms right-handed helical ribbons and crystals.
Lara, Cecile; Reynolds, Nicholas; Berryman, Josh et al.
2014In Journal of the American Chemical Society, 136 (12), p. 4732-4739
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Abstract :
[en] Amyloid fibrils are implicated in over 20 neurodegenerative diseases. The mechanisms of fibril structuring and formation are not only of medical and biological importance but are also relevant for material science and nanotechnologies due to the unique structural and physical properties of amyloids. We previously found that hen egg white lysozyme, homologous to the disease-related human lysozyme, can form left-handed giant ribbons, closing into nanotubes. By using matrix-assisted laser desorption ionization mass spectrometry analysis, we here identify a key component of such structures: the ILQINS hexapeptide. By combining atomic force microscopy and circular dichorism, we find that this fragment, synthesized by solid-phase peptide synthesis, also forms fibrillar structures in water at pH 2. However, all fibrillar structures formed possess an unexpected right-handed twist, a rare chirality within the corpus of amyloid experimental observations. We confirm by small- and wide-angle X-ray scattering and molecular dynamics simulations that these fibrils are composed of conventional left-handed β-sheets, but that packing stresses between adjacent sheets create this twist of unusual handedness. We also show that the right-handed fibrils represent a metastable state toward β-sheet-based microcrystals formation.
Research center :
ULHPC - University of Luxembourg: High Performance Computing
Disciplines :
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Lara, Cecile
Reynolds, Nicholas
Berryman, Josh  ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit
Xu, Anqui
Zhang, Afang
Mezzenga, Raffaele
Language :
English
Title :
ILQINS hexapeptide, identified in lysozyme left-handed helical ribbons and nanotubes, forms right-handed helical ribbons and crystals.
Publication date :
26 March 2014
Journal title :
Journal of the American Chemical Society
ISSN :
1520-5126
Publisher :
American Chemical Society, Washington, United States - District of Columbia
Volume :
136
Issue :
12
Pages :
4732-4739
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 08 May 2014

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