Article (Scientific journals)
Munc13 controls the location and efficiency of dense-core vesicle release in neurons.
van de Bospoort, Rhea; Farina, Margherita; Schmitz, Sabine et al.
2012In The Journal of cell biology, 199 (6), p. 883-91
Peer reviewed
 

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Keywords :
Animals; Cells, Cultured; Hippocampus/physiology; Intracellular Signaling Peptides and Proteins/biosynthesis/genetics/physiology; Mice; Mice, Knockout; Nerve Tissue Proteins/biosynthesis/genetics/physiology; Neurons/physiology; Presynaptic Terminals/physiology; Secretory Vesicles/secretion
Abstract :
[en] Neuronal dense-core vesicles (DCVs) contain diverse cargo crucial for brain development and function, but the mechanisms that control their release are largely unknown. We quantified activity-dependent DCV release in hippocampal neurons at single vesicle resolution. DCVs fused preferentially at synaptic terminals. DCVs also fused at extrasynaptic sites but only after prolonged stimulation. In munc13-1/2-null mutant neurons, synaptic DCV release was reduced but not abolished, and synaptic preference was lost. The remaining fusion required prolonged stimulation, similar to extrasynaptic fusion in wild-type neurons. Conversely, Munc13-1 overexpression (M13OE) promoted extrasynaptic DCV release, also without prolonged stimulation. Thus, Munc13-1/2 facilitate DCV fusion but, unlike for synaptic vesicles, are not essential for DCV release, and M13OE is sufficient to produce efficient DCV release extrasynaptically.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
van de Bospoort, Rhea
Farina, Margherita
Schmitz, Sabine ;  Vrije Universiteit Amsterdam - VU > Functional Genomics
de Jong, Arthur
de Wit, Heidi
Verhage, Matthijs
Toonen, Ruud F.
Language :
English
Title :
Munc13 controls the location and efficiency of dense-core vesicle release in neurons.
Publication date :
2012
Journal title :
The Journal of cell biology
ISSN :
0021-9525
eISSN :
1540-8140
Volume :
199
Issue :
6
Pages :
883-91
Peer reviewed :
Peer reviewed
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since 23 March 2014

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