Reference : Munc13 controls the location and efficiency of dense-core vesicle release in neurons.
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
Munc13 controls the location and efficiency of dense-core vesicle release in neurons.
van de Bospoort, Rhea [> >]
Farina, Margherita [> >]
Schmitz, Sabine mailto [Vrije Universiteit Amsterdam - VU > Functional Genomics]
de Jong, Arthur [> >]
de Wit, Heidi [> >]
Verhage, Matthijs [> >]
Toonen, Ruud F. [> >]
The Journal of cell biology
Yes (verified by ORBilu)
United States
[en] Animals ; Cells, Cultured ; Hippocampus/physiology ; Intracellular Signaling Peptides and Proteins/biosynthesis/genetics/physiology ; Mice ; Mice, Knockout ; Nerve Tissue Proteins/biosynthesis/genetics/physiology ; Neurons/physiology ; Presynaptic Terminals/physiology ; Secretory Vesicles/secretion
[en] Neuronal dense-core vesicles (DCVs) contain diverse cargo crucial for brain development and function, but the mechanisms that control their release are largely unknown. We quantified activity-dependent DCV release in hippocampal neurons at single vesicle resolution. DCVs fused preferentially at synaptic terminals. DCVs also fused at extrasynaptic sites but only after prolonged stimulation. In munc13-1/2-null mutant neurons, synaptic DCV release was reduced but not abolished, and synaptic preference was lost. The remaining fusion required prolonged stimulation, similar to extrasynaptic fusion in wild-type neurons. Conversely, Munc13-1 overexpression (M13OE) promoted extrasynaptic DCV release, also without prolonged stimulation. Thus, Munc13-1/2 facilitate DCV fusion but, unlike for synaptic vesicles, are not essential for DCV release, and M13OE is sufficient to produce efficient DCV release extrasynaptically.

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