Conformational behaviour of a synthetic peptide of the C-terminus of villin that interacts with actin: an NMR, CD and stimulated annealing study.

in International journal of peptide and protein research (1995), 45(6), 574-86

The solution structure of a synthetic 22-amino acid peptide (P1) corresponding to the extreme C-terminal end and one of the F-actin binding sites of villin has been determined by 1H NMR and CD ... [more ▼]

The solution structure of a synthetic 22-amino acid peptide (P1) corresponding to the extreme C-terminal end and one of the F-actin binding sites of villin has been determined by 1H NMR and CD spectroscopy. The structure of this peptide was compared to that of a peptide in which lysine to glutamic acid substitutions were introduced at positions 17 and 19 (P11), abolishing F-actin binding. Both peptides are largely unstructured in aqueous solution. Changes observed in the NMR and CD spectra of both peptides are consistent with alpha-helix formation in trifluoroethanol/water mixtures. A set of 189 interproton distances derived from nuclear Overhauser enhancement (NOE) measurements, 17 phi-angle constraints obtained from 3JNH alpha coupling constants, as well as about 10 N ... O distance restraints deduced from amide proton exchange kinetics with deuterium, were used for the structure determination. The three-dimensional structure of P1 and P11 is characterized by two helical regions, one extending from residues 2 to 5 and a second covering residues 7 to 17. The central fragment, ranging from Leu-7 to Leu-15, is more stable. The C-terminal residues are less structured, particularly within peptide P11. The significance of these structural results is discussed in relation to the biological activity of villin. [less ▲]

Die Wirtschaftswunder in Ostasien, Eine Suche nach Erklärungen

in IFO-Studien (1995)

Novel HEMT layout: The RoundHEMT

in Electronics Letters (1995), 31(1995), 589-591

The Spectrum Efficiency of a Base-Station Antenna Array System for Spatially Selective Transmission

in IEEE Transactions on Vehicular Technology (1995), 44(3), 651660

Validation of ocean tide models by comparison to gravity loading measurements

in Marées Terrestres Bulletin d'Informations (1995), 122

A double-labelling fluorescent assay for concomitant measurements of [Ca2+]i and O2. production in human macrophages

in Biochimica et Biophysica Acta (1995), 1244(1), 79-84

To measure intracellular free Ca2+ concentration ([Ca2+]i) and superoxide (O2) production in human alveolar macrophages, we used the fluorescent Ca2+ indicator fura-2 and the O2-sensitive dye ... [more ▼]

To measure intracellular free Ca2+ concentration ([Ca2+]i) and superoxide (O2) production in human alveolar macrophages, we used the fluorescent Ca2+ indicator fura-2 and the O2-sensitive dye dihydrorhodamine-123, which becomes fluorescent in its oxidized form, rhodamine-123. We describe a new double-dye technique whereby the kinetics of both [Ca2+]i levels and O2. production can be monitored simultaneously. This technique was developed in the dimethylsulfoxide-differentiated monocytic-like U-937 cell line (not equal to U-937), validated by comparison with single dye measurements and applied to human alveolar macrophages. The chemotactic peptide N-formyl-L-Methionyl-L-Leucyl-L-Phenylalanine induced in both cell types a similar transient elevation in [Ca2+]i, followed within seconds by a sustained increase in O2 production, which was however 4-fold weaker in not equal to U-937 cells. These results indicate that O2 production is an early event following the stimulation of human alveolar macrophages. This new double-dye technique may be relevant to other O2 ion-producing cells and could help to define more precisely the kinetics of the events leading to this biological response. [less ▲]

Le français et les langues mélanésiennes – des langues en contact

in Etudes Françaises (1995), (1), 79-85

Interaction between undulated and Patch leads to an extreme form of spina bifida in double-mutant mice.

in Nature Genetics (1995), 11(1), 60-3

The aetiology of spina bifida involves genetic and environmental factors, which may be why major genes contributing to pathogenesis have not been identified. Here we report that undulated-Patch double ... [more ▼]

The aetiology of spina bifida involves genetic and environmental factors, which may be why major genes contributing to pathogenesis have not been identified. Here we report that undulated-Patch double-mutant mice have a phenotype reminiscent of an extreme form of spina bifida occulta in humans. This unexpected phenotype in double-mutant but not single-mutant mice shows that novel congenital anomalies such as spina bifida can result from interaction between products of independently segregating loci. This example of digenic inheritance may explain the often sporadic nature of spina bifida in humans. [less ▲]

The biological cascade leading to cardiac hypertrophy.

in European heart journal (1995), 16 Suppl N

Cardiac hypertrophy, one of the major risk factors in hypertension, is associated with a high incidence of congestive heart failure and sudden death. Despite efforts over the last 20 years, the underlying ... [more ▼]

Cardiac hypertrophy, one of the major risk factors in hypertension, is associated with a high incidence of congestive heart failure and sudden death. Despite efforts over the last 20 years, the underlying molecular mechanisms of cardiac hypertrophy are still poorly understood, thus making it difficult to develop new therapeutic strategies. A growing body of evidence suggests that cardiac hypertrophy results from mechanical stress that triggers paracrine and autocrine signal transduction pathways. Furthermore, whereas hypertrophy leads to isoform switches in some contractile proteins, increased protein synthesis is largely based on increased translational capacity. Cardiac growth under physiological as well as pathological conditions is regulated by several recently identified transcription factors. Among the factors that are capable of transmitting hypertrophic stimuli to the nucleus is the early growth response gene-1 (Egr-1). Whereas female gender is already an established cardioprotective factor in clinical trials, some very recent data indicate that oestrogens and the nuclear oestrogen receptor may directly modulate gene expression in the development of cardiac hypertrophy. Future pharmacological interventions could be directed towards modifying the nuclear signal transduction cascade involving multiple protein kinases and phosphatases. [less ▲]

Cloning and expression analysis of a novel mesodermally expressed cadherin.

in Developmental Biology (1995), 169(1), 337-46

Cadherins are calcium-dependent cell adhesion molecules which show developmental and tissue-specific expression. Here we report the cloning of a mouse cadherin which is predominantly expressed in tissues ... [more ▼]

Cadherins are calcium-dependent cell adhesion molecules which show developmental and tissue-specific expression. Here we report the cloning of a mouse cadherin which is predominantly expressed in tissues of mesodermal origin. In contrast to other cadherins, cadherin-11 expression is largely restricted to mesenchymal tissues surrounding various organs but is not found in epithelia. Sequence analysis suggests that this cadherin is the mouse homologue of the previously reported human cadherin-11 and is a member of a cadherin subfamily, which is evolutionarily distinct from other cadherin subfamilies identified so far. [less ▲]

Das erste Spital in Luxemburg. Eine unerforschte Quelle zum Wirken Walrams von Monschau-Limburg und zur Geschichte der Stadt Luxemburg

in Hemecht : Zeitschrift für Luxemburger Geschichte = Revue d'Histoire Luxembourgeoise (1995), 47(1), 15-42

Urban Space and Logistics. On the road to Sustainability?

in World Transport Policy & Practice (1995), 1(1), 39-45

« Mal di luna (Cyrano de Bergerac, Cavazzoni, Fellini)»

in Romaniac (1995)

Right-wing extremism, racism or youth violence? Explaining violence against foreigners in Germany

in New Community (1995), 21(4), 501-523

A gene for autosomal dominant sacral agenesis maps to the holoprosencephaly region at 7q36.

in Nature Genetics (1995), 11(1), 93-5

Sacral agenesis is a rare disorder of uncertain incidence that has been reported in diverse populations. Although usually sporadic and most commonly associated with maternal diabetes, there is a ... [more ▼]

Sacral agenesis is a rare disorder of uncertain incidence that has been reported in diverse populations. Although usually sporadic and most commonly associated with maternal diabetes, there is a hereditary form which may occur in isolation or with a presacral mass (anterior meningocele and/or presacral teratoma) and anorectal abnormalities, which constitute the Currarino triad (MIM 176450). The radiological hallmark of hereditary sacral agenesis is a hemi-sacrum (sickle-shaped sacrum) with intact first sacral vertebra. Bowel obstruction is the usual neonatal presentation, but, unlike other neural tube defects, adult presentation is not uncommon. The major pathology is confined to the pelvic cavity and may present as a space-occupying lesion or meningitis due to ascending infection. All recurrences in families have been compatible with autosomal dominant inheritance except for those associated with the isomerism gene at Xq24-q27.1 (ref. 3). Several associated cytogenetic defects have been reported, including 7q deletions. Previous studies failed to detect linkage to HLA markers, but we now present evidence for a location on 7q36. The same region also contains a gene for holoprosencephaly, an early malformation of the extreme rostral end of the neural tube. [less ▲]

Rez. zu Hermann Ehmann: Jugendsprache und Dialekt

in Zeitschrift für Deutsche Philologie (1995), 114

«Een Eco-grafie van de cultroman»

in ALW-Cahier (1995)

Universal relaxation behavior of classical liquid crystals at hypersonic frequencies.

in Physical Review. E : Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics (1995), 51

Within this work we present comparative Brillouin and ultrasonic investigations of p-methoxybenzylidene p-(n-butylaniline), p-azoxyanisol, and 4-cyano-4’-pentyl-biphenyl performed in the vicinity of the ... [more ▼]

Within this work we present comparative Brillouin and ultrasonic investigations of p-methoxybenzylidene p-(n-butylaniline), p-azoxyanisol, and 4-cyano-4’-pentyl-biphenyl performed in the vicinity of the nematic-isotropic transition. We confirm the existence of a universal hypersonic relaxation mechanism of weak activation energy and a related significant splitting of the two principal longitudinal acoustic modes within the nematic phase. Brillouin refractometry is proposed as a method to determine the magnitude of the nematic order parameter simultaneously with the second-order elastic stiffness constants. The origin of the hypersonic anisotropy is discussed in terms of thermal relaxations and the evolution of the nematic order parameter. [less ▲]

Observations - La tierce complicité de la violation d'un pacte de préférence notifié à la société (note sous Cass. fr., 26 avril 1994) (n° 6664)

in Revue Pratique des Sociétés Civiles et Commerciales (1995), (1), 59

Frauen im pädagogischen Diskurs. Eine interdisziplinäre Biblio­graphie. 1988-1993. Frankfurt: Ulrike Helmer 1994

in Feministische Studien (1995), 13(2), 160-162

Algèbre de Schwartz d'un groupe de Lie nilpotent

in Travaux Mathématiques (1995), 7

Von Mäusen und Menschen In: “Genetische Determinierung: Schicksal aus den Genen?”

in GSF-Mensch und Umwelt (1995), (10), 17-23

Lange Wellen“ der Unternehmenskonjunktur: Eine Untersuchung langfristiger Umsatz- und Innovationszyklen der Druckmaschinenfabrik Koenig & Bauer in Würzburg (1817-1992)

in Zeitschrift für Unternehmensgeschichte (1995)

Führt Ängstlichkeit und Ärgerneigung bei Leistungssportlern zu erhöhter Stressanfälligkeit?

in Psychologie und Sport (1995), 2(1), 14-20

Abhängig von dem Ausmaß der personenspezifischen Streßanfälligkeit sind in emotional belastenden Situationen unterschiedliche Reaktionsweisen mit häufig negativen Folgen auf die sportliche Leistung ... [more ▼]

Abhängig von dem Ausmaß der personenspezifischen Streßanfälligkeit sind in emotional belastenden Situationen unterschiedliche Reaktionsweisen mit häufig negativen Folgen auf die sportliche Leistung feststellbar. Die vorliegende Studie an luxemburgischen Leistungssportlerinnen und -sportlern zeigt auf, inwieweit Ängstlichkeit, Ärgerneigung sowie Formen des Ärgerausdrucks und der Streßverarbeitung als Prädiktoren der Streßanfälligkeit von Bedeutung sind. Insbesondere der Befund, daß bei hoher Ärgerneigung ein geringer aktiver Kontrollversuch die Streßanfälligkeit erhöht, wird hinsichtlich seiner Bedeutung für den Leistungssport diskutiert. [less ▲]

EXPLORING THE MYCOPLASMA-CAPRICOLUM GENOME - A MINIMAL CELL REVEALS ITS PHYSIOLOGY

in Molecular Microbiology (1995), 16(5), 955-967

We report on the analysis of 214 kb of the parasitic eubacterium Mycoplasma capricolum sequenced by genomic walking techniques. The 287 putative proteins detected to date represent about half of the ... [more ▼]

We report on the analysis of 214 kb of the parasitic eubacterium Mycoplasma capricolum sequenced by genomic walking techniques. The 287 putative proteins detected to date represent about half of the estimated total number of 500 predicted for this organism. A large fraction of these (75%) can be assigned a likely function as a result of similarity searches. Several important features of the functional organization of this small genome are already apparent. Among these are (i) the expected relatively large number of enzymes involved in metabolic transport and activation, for efficient use of host cell nutrients; (ii) the presence of anabolic enzymes; (iii) the unexpected diversity of enzymes involved in DNA replication and repair; and (iv) a sizeable number of orthologues (82 so far) in Escherichia coil. This survey is beginning to provide a detailed view of how M. capricolum manages to maintain essential cellular processes with a genome much smaller than that of its bacterial relatives. [less ▲]

Array Processing in Correlated Noise Fields Based on Instrumental Variables and Subspace Fitting

in IEEE Transactions on Signal Processing (1995), 43

Psychologische Trainigs für die Praxis - Darstellung zweier Verfahren

in Psychologie und Sport -Schorndorf- (1995), 2(3), 1-5

Fast and sensitive search of information databases for biological relationships

in Statustagung des BMBF, HPSC 95, Stand und Perspektiven des Parallelen Höchstleistungsrechnens und seiner Anwendungen (1995)

Sequence comparison has become an essential and standard tool in the analysis of genomic data. Genome projects will decipher much of the genetic information in many organisms, including humans. As a ... [more ▼]

Sequence comparison has become an essential and standard tool in the analysis of genomic data. Genome projects will decipher much of the genetic information in many organisms, including humans. As a result, the computational cost of databank searches will increase dramatically. In addition, the search for biologically meaningful homology between a newly determined sequence and sequences already stored in the various databanks becomes increasingly important as most of the new data will be in raw, not understood form. The detection of sufficient similarity between a newly determined sequence to a protein of know function or even known 3D-structure in a databank allows one to transfer most of the knowledge from one sequence to the other. The result can be enormous savings in genetic and biochemical laboratory efforts. [less ▲]

Development, Pattern and Causes of Violence against Foreigners in Germany: Social and Biographical Characteristics of Perpetrators and the Process of Escalation

in Terrorism & Political Violence (1995), 7(1), 162-181

S.A.S. : un exemple à suivre ?

in Le droit des affaires = Het ondernemingsrecht (1994), (33), 43-80

Pouvoir princier et peuplement : aux origines de la ville de Luxembourg

in Château Gaillard (1994), 16

Teststatistische Überprüfung der Impact of Event-Skala: Befunde zu Reliabilität und Stabilität.

in Focus Diagnostica (1994), 40(4)

Dossier Dewey. Het ontwerp van een transactionele handelingspsychologie.

in Psychologie & maatschappij (1994), 18(2), 123-140

Phosphorus Redistribution During the Formation of Buried CoSi2 Layers by Ion Beam Synthesis,

in Nuclear Instruments and Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms (1994), 84

«Nos w nos z humorem»

in Humor Europejski (1994)

Testicular self-examination: Attitudes and practices among young men in Europe

in Preventive Medicine (1994), 23

BACKGROUND: Testicular self examination (TSE) is recommended for the early detection of testicular cancer. Evidence from North America suggests there is only limited public awareness of its importance ... [more ▼]

BACKGROUND: Testicular self examination (TSE) is recommended for the early detection of testicular cancer. Evidence from North America suggests there is only limited public awareness of its importance among the young male population. Compliance with regular TSE is found in only a small minority of young men. Attitudes toward and practice of TSE have rarely been studied outside North America. METHOD: Attitudes to TSE were evaluated by questionnaire in a sample of 16,486 students. Frequency of TSE practice was reported by the 7,304 men in the sample. The data were collected as part of the European Health Behavior Survey, an international study on health beliefs and health behavior. RESULTS: Eighty-seven percent of men reported never having practiced TSE. Regular practice (monthly) was reported by only 3% of the sample, with another 10% reporting occasional TSE. Significant differences emerged between countries, ranging from 76% of German men to 98% of Icelandic men reporting no TSE. Men rated TSE as less important to health than women. Attitude toward TSE among men was a significant predictor of TSE practice. CONCLUSION: Both the low levels of TSE and the low ratings of the importance of TSE suggest that young men in Europe are unaware of the value of this comparatively simple method of early detection of cancer. If a highly educated population group in the "at risk" age category is not carrying out the recommendations, it is unlikely that there are higher levels of compliance in other groups. These results suggest an important role for health education in the early detection of testicular cancer. [less ▲]

NADPH-diaphorase reactivity in articular afferents of a normal and inflamed knee joint in the cat.

in Brain Research (1994), 668

Nichts als Ärger. Zur Modifikation von Ärgerreaktionen im Leistungssport

in Leistungssport (1994), 24(3), 27-30

Ein Fußballspieler, der aufgrund seiner "Wut im Bauch" ein Revanchefoul begeht, oder ein anderer, der aufgrund seines Ärgers das Siegestor erzielt, sind Beispiele für höchst unterschiedliche Auswirkungen ... [more ▼]

Ein Fußballspieler, der aufgrund seiner "Wut im Bauch" ein Revanchefoul begeht, oder ein anderer, der aufgrund seines Ärgers das Siegestor erzielt, sind Beispiele für höchst unterschiedliche Auswirkungen der Emotion Ärger. Im Kontext leistungssportlichen Handelns wird von Sportlern, Trainern, Betreuern und Sportwissenschaftlern die leistungsfördernde Wirkung von Ärgererleben heftig diskutiert. Um die Kontroversen zu entschärfen, wird in diesem Beitrag eine psychologische Sichtweise zum Thema "Ärger" dargelegt. Die Gründe für eine Veränderung von Ärgerreaktionen, ein theoretischer Erklärungsansatz sowie ein Modifikationsprogramm des Ärgers werden hierzu vorgestellt. Ziel ist es, ein differenzierteres Bild der Veränderungsnotwendigkeiten und -möglichkeiten von Ärgerreaktionen aufzuzeigen. [less ▲]

Institutionelle Unsicherheit und wirtschaftliche Entwicklung

in Jahrbucher für Nationalokonomie und Statistik (1994)

Education as Practical Intersubjectivity. Towards a critical-pragmatic understanding of education.

in Educational Theory (1994), 44(3), 299-317

Fremdenfeindliche Gewalt: Tätertypen, Eskalationsprozesse und Erklärungsansätze

in Politisches Lernen (1994), (1-2), 11-25

Le projet de dictionnaire biographique du mouvement ouvrier luxembourgeois

in Matériaux pour l'histoire de notre temps (1994), (34), 47-49

PHD - AN AUTOMATIC MAIL SERVER FOR PROTEIN SECONDARY STRUCTURE PREDICTION

in Computer Applications in the Biosciences [=CABIOS] (1994), 10(1), 53-60

By the middle of 1993, > 30000 protein sequences had been listed. For 1000 of these, the three-dimensional (tertiary) structure has been experimentally solved. Another 7000 can be modelled by homology ... [more ▼]

By the middle of 1993, > 30000 protein sequences had been listed. For 1000 of these, the three-dimensional (tertiary) structure has been experimentally solved. Another 7000 can be modelled by homology. For the remaining 21000 sequences, secondary structure prediction provides a rough estimate of structural features. Predictions in three states range between 35% (random) and 88% (homology modelling) overall accuracy. Using information about evolutionary conservation as contained in multiple sequence alignments, the secondary structure of 4700 protein sequences was predicted by the automatic e-mail sewer PHD, For proteins with at least one known homologue, the method has an expected overall three-state accuracy of 71.4% for proteins with at least one known homologue (evaluated on 126 unique protein chains). [less ▲]

THE HSSP DATABASE OF PROTEIN-STRUCTURE SEQUENCE ALIGNMENTS

in Nucleic Acids Research (1994), 22(17), 3597-3599

HSSP (homology-derived structures of proteins) is a derived database merging structural (2-D and 3-D) and sequence information (1-D). For each protein of known 3D structure from the Protein Data Bank, the ... [more ▼]

HSSP (homology-derived structures of proteins) is a derived database merging structural (2-D and 3-D) and sequence information (1-D). For each protein of known 3D structure from the Protein Data Bank, the database has a file with all sequence homologues, properly aligned to the PDB protein. Homologues are very likely to have the same 3D structure as the PDB protein to which they have been aligned. As a result, the database is not only a database of sequence aligned sequence families, but it is also a database of implied secondary and tertiary structures. [less ▲]

Pragmatism as a pedagogy of communicative action.

in Studies in Philosophy & Education (1994), 13

Detection of atmospheric pressure loading using Very Long Baseline Interferometry measurements

in Journal of Geophysical Research (1994), 99(B3), 4505-4518

Loading of the Earth by the temporal redistribution of global atmospheric mass is likely to displace the positions of geodetic monuments by tens of millimeters both vertically and horizontally. Estimates ... [more ▼]

Loading of the Earth by the temporal redistribution of global atmospheric mass is likely to displace the positions of geodetic monuments by tens of millimeters both vertically and horizontally. Estimates of these displacements are determined by convolving National Meteorological Center (NMC) global values of atmospheric surface pressure with Farrell's elastic Green's functions. An analysis of the distances between radio telescopes determined by very long baseline interferometry (VLBI) between 1984 and 1992 reveals that in many of the cases studied there is a significant contribution to baseline length change due to atmospheric pressure loading. Our analysis covers intersite distances of between 1000 and 10,000 km and is restricted to those baselines measured more than 100 times. Accounting for the load effects (after first removing a best fit slope) reduces the weighted root-mean-square (WRMS) scatter of the baseline length residuals on 11 of the 22 baselines investigated. The slight degradation observed in the WRMS scatter on the remaining baselines is largely consistent with the expected statistical fluctuations when a small correction is applied to a data set having a much larger random noise. The results from all baselines are consistent with ∼60% of the computed pressure contribution being present in the VLBI length determinations. Site dependent coefficients determined by fitting local pressure to the theoretical radial displacement are found to reproduce the deformation caused by the regional pressure to within 25% for most inland sites. The coefficients are less reliable at near coastal and island stations. [less ▲]

Gute Noten. Der Schüler Kleist in den Aufzeichnungen des Carl Eduard Albanus

in Brandenburger Kleist-Blätter (1994), 7

REDEFINING THE GOALS OF PROTEIN SECONDARY STRUCTURE PREDICTION

in Journal of Molecular Biology (1994), 235(1), 13-26

Observations sous Cass., 20 janvier 1994 (n° 6653)

in Revue Pratique des Sociétés Civiles et Commerciales (1994), (4), 408-409

Commentaire de l'une des rares décisions précisant que la règle stipulant l'irrecevabilité de l'action en justice d'une société demeurée en défaut de procéder au dépôt de son acte constitutif au greffe du ... [more ▼]

Commentaire de l'une des rares décisions précisant que la règle stipulant l'irrecevabilité de l'action en justice d'une société demeurée en défaut de procéder au dépôt de son acte constitutif au greffe du tribunal compétent n'est pas d'ordre public [less ▲]

Observations - Les apparences dans le cadre d'une action intentée contre les membres d'une association de fait : de la manière de les sauver ou, au contraire, de l'invitation à s'en méfier ? Ou de l'incidence des mystères en droit (n° 6636)

in Revue Pratique des Sociétés Civiles et Commerciales (1994), (2), 133-167