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See detailConformational behaviour of a synthetic peptide of the C-terminus of villin that interacts with actin: an NMR, CD and stimulated annealing study.
Simenel, C.; Rose, T.; Goethals, M. et al

in International journal of peptide and protein research (1995), 45(6), 574-86

The solution structure of a synthetic 22-amino acid peptide (P1) corresponding to the extreme C-terminal end and one of the F-actin binding sites of villin has been determined by 1H NMR and CD ... [more ▼]

The solution structure of a synthetic 22-amino acid peptide (P1) corresponding to the extreme C-terminal end and one of the F-actin binding sites of villin has been determined by 1H NMR and CD spectroscopy. The structure of this peptide was compared to that of a peptide in which lysine to glutamic acid substitutions were introduced at positions 17 and 19 (P11), abolishing F-actin binding. Both peptides are largely unstructured in aqueous solution. Changes observed in the NMR and CD spectra of both peptides are consistent with alpha-helix formation in trifluoroethanol/water mixtures. A set of 189 interproton distances derived from nuclear Overhauser enhancement (NOE) measurements, 17 phi-angle constraints obtained from 3JNH alpha coupling constants, as well as about 10 N ... O distance restraints deduced from amide proton exchange kinetics with deuterium, were used for the structure determination. The three-dimensional structure of P1 and P11 is characterized by two helical regions, one extending from residues 2 to 5 and a second covering residues 7 to 17. The central fragment, ranging from Leu-7 to Leu-15, is more stable. The C-terminal residues are less structured, particularly within peptide P11. The significance of these structural results is discussed in relation to the biological activity of villin. [less ▲]

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See detailDie Wirtschaftswunder in Ostasien, Eine Suche nach Erklärungen
Klump, Rainer UL; Menkhoff, Lukas

in IFO-Studien (1995)

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See detailPanorama de jurisprudence en matière d'indemnisation du dommage
Ravarani, Georges UL

in Pasicrisie Luxembourgeoise: Recueil Trimestriel de la Jurisprudence Luxembourgeoise (1995), (29), 153232

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See detailNovel HEMT layout: The RoundHEMT
Marso, Michel UL; Schimpf, K.; Fox, A. et al

in Electronics Letters (1995), 31(1995), 589-591

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See detailThe Spectrum Efficiency of a Base-Station Antenna Array System for Spatially Selective Transmission
Zetterberg, Per; Ottersten, Björn UL

in IEEE Transactions on Vehicular Technology (1995), 44(3), 651660

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See detailPostmodernism and the repoliticization of education.
Biesta, Gert UL

in Interchange (1995), 26

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See detailValidation of ocean tide models by comparison to gravity loading measurements
Francis, Olivier UL

in Marées Terrestres Bulletin d'Informations (1995), 122

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See detailWandlungen politisch motivierter Gewalt in der Bundesrepublik
Willems, Helmut UL; Eckert, Roland

in Gruppendynamik (1995), 26. Jg(1), 89-123

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See detailA double-labelling fluorescent assay for concomitant measurements of [Ca2+]i and O2. production in human macrophages
Bueb, Jean-Luc UL; Gallois, A.; Schneider, J. C. et al

in Biochimica et Biophysica Acta (1995), 1244(1), 79-84

To measure intracellular free Ca2+ concentration ([Ca2+]i) and superoxide (O2) production in human alveolar macrophages, we used the fluorescent Ca2+ indicator fura-2 and the O2-sensitive dye ... [more ▼]

To measure intracellular free Ca2+ concentration ([Ca2+]i) and superoxide (O2) production in human alveolar macrophages, we used the fluorescent Ca2+ indicator fura-2 and the O2-sensitive dye dihydrorhodamine-123, which becomes fluorescent in its oxidized form, rhodamine-123. We describe a new double-dye technique whereby the kinetics of both [Ca2+]i levels and O2. production can be monitored simultaneously. This technique was developed in the dimethylsulfoxide-differentiated monocytic-like U-937 cell line (not equal to U-937), validated by comparison with single dye measurements and applied to human alveolar macrophages. The chemotactic peptide N-formyl-L-Methionyl-L-Leucyl-L-Phenylalanine induced in both cell types a similar transient elevation in [Ca2+]i, followed within seconds by a sustained increase in O2 production, which was however 4-fold weaker in not equal to U-937 cells. These results indicate that O2 production is an early event following the stimulation of human alveolar macrophages. This new double-dye technique may be relevant to other O2 ion-producing cells and could help to define more precisely the kinetics of the events leading to this biological response. [less ▲]

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See detailAPO-I-mediated apoptosis in normal and malignant lymphocytes
Dhein, J.; Behrmann, Iris UL; Daniel, P. T. et al

in Biochemical Society Transactions (1995), 22(3), 598-600

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See detailLe français et les langues mélanésiennes – des langues en contact
Ehrhart, Sabine UL

in Etudes Françaises (1995), (1), 79-85

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See detailStressimpfungstraining
Steffgen, Georges UL; de Boer, Claudia

in Psychologie und Sport -Schorndorf- (1995), 2(3), 83-86

Das Streßimpfungstraining (SIT) nach Meichenbaum (1991) stellt ein Verfahren dar, durch das gelernt wird, Streßsituationen bzw. emotional belastende Ereignisse angemessen zu bewältigen. Trainiert werden ... [more ▼]

Das Streßimpfungstraining (SIT) nach Meichenbaum (1991) stellt ein Verfahren dar, durch das gelernt wird, Streßsituationen bzw. emotional belastende Ereignisse angemessen zu bewältigen. Trainiert werden allgemein anwendbare Strategien. Einheitliches Merkmal dieser Strategien ist die Kompetenz, Problem- bzw. Streßsituationen frühzeitig zu erkennen und diese Situationen durch alternative/kognitive und verhaltensbezogene Bewältigungsmaßnahmen zu meistern. [less ▲]

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See detailInteraction between undulated and Patch leads to an extreme form of spina bifida in double-mutant mice.
Helwig, U.; Imai, K.; Schmahl, W. et al

in Nature Genetics (1995), 11(1), 60-3

The aetiology of spina bifida involves genetic and environmental factors, which may be why major genes contributing to pathogenesis have not been identified. Here we report that undulated-Patch double ... [more ▼]

The aetiology of spina bifida involves genetic and environmental factors, which may be why major genes contributing to pathogenesis have not been identified. Here we report that undulated-Patch double-mutant mice have a phenotype reminiscent of an extreme form of spina bifida occulta in humans. This unexpected phenotype in double-mutant but not single-mutant mice shows that novel congenital anomalies such as spina bifida can result from interaction between products of independently segregating loci. This example of digenic inheritance may explain the often sporadic nature of spina bifida in humans. [less ▲]

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See detailSteigern psychische Belastungen das Eßbedürfnis? Eine experimentelle Studie an Bulimikerinnen
Tuschen-Caffier, Brunna; Vögele, Claus UL; Kuhnhardt, Karin et al

in Zeitschrift für Klinische Psychologie, Psychopathologie und Psychotherapie (1995), 24

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See detailThe biological cascade leading to cardiac hypertrophy.
Neyses, Ludwig UL; Pelzer, T.

in European heart journal (1995), 16 Suppl N

Cardiac hypertrophy, one of the major risk factors in hypertension, is associated with a high incidence of congestive heart failure and sudden death. Despite efforts over the last 20 years, the underlying ... [more ▼]

Cardiac hypertrophy, one of the major risk factors in hypertension, is associated with a high incidence of congestive heart failure and sudden death. Despite efforts over the last 20 years, the underlying molecular mechanisms of cardiac hypertrophy are still poorly understood, thus making it difficult to develop new therapeutic strategies. A growing body of evidence suggests that cardiac hypertrophy results from mechanical stress that triggers paracrine and autocrine signal transduction pathways. Furthermore, whereas hypertrophy leads to isoform switches in some contractile proteins, increased protein synthesis is largely based on increased translational capacity. Cardiac growth under physiological as well as pathological conditions is regulated by several recently identified transcription factors. Among the factors that are capable of transmitting hypertrophic stimuli to the nucleus is the early growth response gene-1 (Egr-1). Whereas female gender is already an established cardioprotective factor in clinical trials, some very recent data indicate that oestrogens and the nuclear oestrogen receptor may directly modulate gene expression in the development of cardiac hypertrophy. Future pharmacological interventions could be directed towards modifying the nuclear signal transduction cascade involving multiple protein kinases and phosphatases. [less ▲]

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See detailChromosomal localization of the murine cadherin-11.
Hoffman, I.; Balling, Rudi UL

in Mammalian Genome (1995), 6(4), 304

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See detailCloning and expression analysis of a novel mesodermally expressed cadherin.
Hoffmann, I.; Balling, Rudi UL

in Developmental Biology (1995), 169(1), 337-46

Cadherins are calcium-dependent cell adhesion molecules which show developmental and tissue-specific expression. Here we report the cloning of a mouse cadherin which is predominantly expressed in tissues ... [more ▼]

Cadherins are calcium-dependent cell adhesion molecules which show developmental and tissue-specific expression. Here we report the cloning of a mouse cadherin which is predominantly expressed in tissues of mesodermal origin. In contrast to other cadherins, cadherin-11 expression is largely restricted to mesenchymal tissues surrounding various organs but is not found in epithelia. Sequence analysis suggests that this cadherin is the mouse homologue of the previously reported human cadherin-11 and is a member of a cadherin subfamily, which is evolutionarily distinct from other cadherin subfamilies identified so far. [less ▲]

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See detailA simple method for a specific retrograde labelling of dorsal root and sympathetic ganglion cells innervating the knee joint of the cat.
Hanesch, Ulrike UL; Heppelmann, Bernd

in Journal of Neuroscience Methods (1995), 63

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See detailDas erste Spital in Luxemburg. Eine unerforschte Quelle zum Wirken Walrams von Monschau-Limburg und zur Geschichte der Stadt Luxemburg
Margue, Michel UL; Pauly, Michel UL

in Hemecht : Zeitschrift für Luxemburger Geschichte = Revue d'Histoire Luxembourgeoise (1995), 47(1), 15-42

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See detailUrban Space and Logistics. On the road to Sustainability?
Hesse, Markus UL

in World Transport Policy & Practice (1995), 1(1), 39-45

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See detailJacobiennes de certaines courbes de genre 2 : torsion et simplicité
Leprévost, Franck UL

in Journal de Théorie des Nombres de Bordeaux (1995), 7

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See detail« Mal di luna (Cyrano de Bergerac, Cavazzoni, Fellini)»
Roelens, Nathalie UL

in Romaniac (1995)

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See detailA Subspace Fitting Method for Identification of Linear State Space Models
Swindlehurst, A. L.; Roy, R.; Ottersten, Björn UL et al

in IEEE Transactions on Automatic Control (1995)

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See detailRight-wing extremism, racism or youth violence? Explaining violence against foreigners in Germany
Willems, Helmut UL

in New Community (1995), 21(4), 501-523

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See detailPax-1, a regulator of sclerotome development is induced by notochord and floor plate signals in avian embryos.
Ebensperger, C.; Wilting, J.; Brand-Saberi, B. et al

in Anatomy & Embryology (1995), 191(4), 297-310

Pax-1 encodes for a DNA-binding transcriptional activator that was originally discovered in murine embryos using a probe from the Drosophila paired-box-containing gene, gooseberry-distal. We have cloned ... [more ▼]

Pax-1 encodes for a DNA-binding transcriptional activator that was originally discovered in murine embryos using a probe from the Drosophila paired-box-containing gene, gooseberry-distal. We have cloned the avian Pax-1 gene as a basis for experimental studies of the induction of Pax-1 in the paraxial mesoderm. The amino acid sequence of the paired-domain is exactly the same in the quail and mouse, whereas outside the paired-domain there is 61% homology. Starting at about the eight-somite stage, quail Pax-1 is expressed in the paraxial mesoderm in a craniocaudal sequence. The unsegmented paraxial mesoderm and the two most recently formed somites do not express Pax-1. In the epithelial somite, the somitocoele cells and the cells of the ventral two-thirds of the epithelial wall are positive. As soon as the sclerotome is formed, only a subset of sclerotome cells expresses Pax-1. These are the cells that migrate towards the notochord to form the perinotochordal tube. Expression then becomes restricted to the intervertebral discs, the perichondrium of the vertebral bodies and the connective tissue surrounding the spinal ganglia. Additional expression domains are found in the scapula and the pelvic region, distinct areas of the head, and the epithelium of the second to the fourth visceral pouch. In later stages the thymus is positive. In vitro and in vivo experiments show that the notochord induces Pax-1 in the paraxial mesoderm, but limb bud mesoderm is not competent to respond to notochordal signals. Floor plate is also capable of inducing Pax-1 expression in sclerotome cells. Our studies show that in competent cells of the paraxial mesoderm, Pax-1 is a mediator of signals emanating from the notochord and the floor plate. [less ▲]

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See detailA gene for autosomal dominant sacral agenesis maps to the holoprosencephaly region at 7q36.
Lynch, S. A.; Bond, P. M.; Copp, A. J. et al

in Nature Genetics (1995), 11(1), 93-5

Sacral agenesis is a rare disorder of uncertain incidence that has been reported in diverse populations. Although usually sporadic and most commonly associated with maternal diabetes, there is a ... [more ▼]

Sacral agenesis is a rare disorder of uncertain incidence that has been reported in diverse populations. Although usually sporadic and most commonly associated with maternal diabetes, there is a hereditary form which may occur in isolation or with a presacral mass (anterior meningocele and/or presacral teratoma) and anorectal abnormalities, which constitute the Currarino triad (MIM 176450). The radiological hallmark of hereditary sacral agenesis is a hemi-sacrum (sickle-shaped sacrum) with intact first sacral vertebra. Bowel obstruction is the usual neonatal presentation, but, unlike other neural tube defects, adult presentation is not uncommon. The major pathology is confined to the pelvic cavity and may present as a space-occupying lesion or meningitis due to ascending infection. All recurrences in families have been compatible with autosomal dominant inheritance except for those associated with the isomerism gene at Xq24-q27.1 (ref. 3). Several associated cytogenetic defects have been reported, including 7q deletions. Previous studies failed to detect linkage to HLA markers, but we now present evidence for a location on 7q36. The same region also contains a gene for holoprosencephaly, an early malformation of the extreme rostral end of the neural tube. [less ▲]

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See detailAnalysis of data from the 1994 international comparison of absolute gravimeters with a single computational protocol
Sasagawa, G.; Klopping, F.; van Dam, Tonie UL

in Metrologia (1995), 32

Data from the 1994 International Comparison of Absolute Gravimeters were processed with the National Oceanic and Atmospheric Administration (NOAA) Geosciences Lab protocols and processing algorithms. The ... [more ▼]

Data from the 1994 International Comparison of Absolute Gravimeters were processed with the National Oceanic and Atmospheric Administration (NOAA) Geosciences Lab protocols and processing algorithms. The rootmean-square discrepancy between results obtained from individual groups and the NOAA processing is 2,3 μGal; the average discrepancy is 1,5 μGal. The sources of the discrepancies are probably (i) differences in the vertical gradients used for datum level transfers, (ii) inconsistencies between processing algorithms, and (iii) differences in data editing criteria. [less ▲]

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See detailRez. zu Hermann Ehmann: Jugendsprache und Dialekt
Sieburg, Heinz UL

in Zeitschrift für Deutsche Philologie (1995), 114

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See detailIndustrial linear optimization problems solved by constraint logic programming
Bisdorff, Raymond UL; Laurent, S.

in European Journal of Operational Research (1995), 84(1), 82-95

In this article we illustrate that Constraint Logic Programming (CLP) systems allow easy expression and solutions of constrained decision problems. CLP solutions for two industrial linear optimization ... [more ▼]

In this article we illustrate that Constraint Logic Programming (CLP) systems allow easy expression and solutions of constrained decision problems. CLP solutions for two industrial linear optimization problems are discussed: - a mixed linear multiple criteria selection problem implemented in the linear rational Prolog III solver, and - a linear integer multiple criteria location problem, particularly adapted to the CHIP solver. [less ▲]

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See detail«Een Eco-grafie van de cultroman»
Roelens, Nathalie UL

in ALW-Cahier (1995)

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See detailKritische onderwijssociologie - vijftien jaar na dato (besprekingsartikel).
Biesta, Gert UL

in Pedagogisch Tijdschrift: Forum voor Opvoedkunde (1995), 20

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See detailUniversal relaxation behavior of classical liquid crystals at hypersonic frequencies.
Grammes, C.; Krüger, Jan-Kristian UL; Bohn, K.-P. et al

in Physical Review. E : Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics (1995), 51

Within this work we present comparative Brillouin and ultrasonic investigations of p-methoxybenzylidene p-(n-butylaniline), p-azoxyanisol, and 4-cyano-4’-pentyl-biphenyl performed in the vicinity of the ... [more ▼]

Within this work we present comparative Brillouin and ultrasonic investigations of p-methoxybenzylidene p-(n-butylaniline), p-azoxyanisol, and 4-cyano-4’-pentyl-biphenyl performed in the vicinity of the nematic-isotropic transition. We confirm the existence of a universal hypersonic relaxation mechanism of weak activation energy and a related significant splitting of the two principal longitudinal acoustic modes within the nematic phase. Brillouin refractometry is proposed as a method to determine the magnitude of the nematic order parameter simultaneously with the second-order elastic stiffness constants. The origin of the hypersonic anisotropy is discussed in terms of thermal relaxations and the evolution of the nematic order parameter. [less ▲]

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See detailSymmetry Lie algebra of the two body systems with a Dirac oscillator interaction
del Sol Mesa, Antonio UL

in Revista Mexicana de Física (1995)

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See detailAlgèbre de Schwartz d'un groupe de Lie nilpotent
Ludwig, Jean; Molitor-Braun, Carine UL

in Travaux Mathématiques (1995), 7

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See detailSecond-order elasticity of liquid crystals within their nematic state at high frequencies
Krüger, Jan-Kristian UL; Grammes, C.; Jiménez, R. et al

in Physical Review. E : Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics (1995), 51(3), 2115-2128

Within this work we present Brillouin and ultrasonic investigations performed on the liquid crystals p-methoxybenzylidene p-(n-butylaniline) (MBBA), p-azoxyanisol (PAA), 4-cyano-4-n-alkylbiphenyles (nCB ... [more ▼]

Within this work we present Brillouin and ultrasonic investigations performed on the liquid crystals p-methoxybenzylidene p-(n-butylaniline) (MBBA), p-azoxyanisol (PAA), 4-cyano-4-n-alkylbiphenyles (nCB), and 4-n-pentoxybenzylidene-4'-n-octoaniline (5O.8). Special attention has been paid to the evolution of a significant splitting of the two longitudinal polarized acoustic modes within the nematic phase. Angle-resolving Brillouin spectroscopy allowed the determination of the complete stiffness tensors and hence a discussion of the propagation of quasitransverse polarized acoustic modes in the nematic state of liquid crystals. We analyze the behavior of the eigenvectors of the relevant acoustic modes and discuss their significance for the propagation of quasishear modes. For 5O.8 we report an acoustic instability within its nematic state. We confirm the existence of a universal thermal relaxation mechanism of weak activation energy for the nematic state of classical liquid crystals. The observed hypersonic anisotropy is discussed in terms of that relaxation mechanism and is compared with that of glass-forming liquid crystals. [less ▲]

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See detailVon Mäusen und Menschen In: “Genetische Determinierung: Schicksal aus den Genen?”
Balling, Rudi UL

in GSF-Mensch und Umwelt (1995), (10), 17-23

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See detailDie Kleist-Sammlung in der Amerika-Gedenkbibliothek, Berlin
Amann, Wilhelm UL

in Beiträge zur Kleist-Forschung (1995), 9

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See detailFührt Ängstlichkeit und Ärgerneigung bei Leistungssportlern zu erhöhter Stressanfälligkeit?
Steffgen, Georges UL; Schwenkmezger, Peter

in Psychologie und Sport (1995), 2(1), 14-20

Abhängig von dem Ausmaß der personenspezifischen Streßanfälligkeit sind in emotional belastenden Situationen unterschiedliche Reaktionsweisen mit häufig negativen Folgen auf die sportliche Leistung ... [more ▼]

Abhängig von dem Ausmaß der personenspezifischen Streßanfälligkeit sind in emotional belastenden Situationen unterschiedliche Reaktionsweisen mit häufig negativen Folgen auf die sportliche Leistung feststellbar. Die vorliegende Studie an luxemburgischen Leistungssportlerinnen und -sportlern zeigt auf, inwieweit Ängstlichkeit, Ärgerneigung sowie Formen des Ärgerausdrucks und der Streßverarbeitung als Prädiktoren der Streßanfälligkeit von Bedeutung sind. Insbesondere der Befund, daß bei hoher Ärgerneigung ein geringer aktiver Kontrollversuch die Streßanfälligkeit erhöht, wird hinsichtlich seiner Bedeutung für den Leistungssport diskutiert. [less ▲]

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See detailPossible Involvement of Gaba-B receptors in the sedative effects of nicotine in mice
Mancuso, Giovanna UL; Tirelli, Ezio

in Journal of Psychopharmacology (1995), 9(3), 51

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See detailEXPLORING THE MYCOPLASMA-CAPRICOLUM GENOME - A MINIMAL CELL REVEALS ITS PHYSIOLOGY
BORK, P.; OUZOUNIS, C.; CASARI, G. et al

in Molecular Microbiology (1995), 16(5), 955-967

We report on the analysis of 214 kb of the parasitic eubacterium Mycoplasma capricolum sequenced by genomic walking techniques. The 287 putative proteins detected to date represent about half of the ... [more ▼]

We report on the analysis of 214 kb of the parasitic eubacterium Mycoplasma capricolum sequenced by genomic walking techniques. The 287 putative proteins detected to date represent about half of the estimated total number of 500 predicted for this organism. A large fraction of these (75%) can be assigned a likely function as a result of similarity searches. Several important features of the functional organization of this small genome are already apparent. Among these are (i) the expected relatively large number of enzymes involved in metabolic transport and activation, for efficient use of host cell nutrients; (ii) the presence of anabolic enzymes; (iii) the unexpected diversity of enzymes involved in DNA replication and repair; and (iv) a sizeable number of orthologues (82 so far) in Escherichia coil. This survey is beginning to provide a detailed view of how M. capricolum manages to maintain essential cellular processes with a genome much smaller than that of its bacterial relatives. [less ▲]

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See detailInP/InGaAs Photodetector Based on a High Electron Mobility Transistor Layer Structure: Its Response at 1.3 µm wavelength
Horstmann, M.; Marso, Michel UL; Fox, A. et al

in Applied Physics Letters (1995), 67(1995), 106-108

We report on the investigation of the room-temperature optoelectronic behavior of a metal–semiconductor–metal two-dimensional electron gas photodiode based on the two-dimensional electron gas of a high ... [more ▼]

We report on the investigation of the room-temperature optoelectronic behavior of a metal–semiconductor–metal two-dimensional electron gas photodiode based on the two-dimensional electron gas of a high electron mobility transistor structure. The photodetector is fabricated in the InP/InGaAs material system, without use of Al-containing layers. Optoelectronic measurements on a device with a finger spacing of 3 mm show a full width at half-maximum (FWHM) of the pulse response of <60 ps, which is the resolution limit of our measurement equipment. Low-temperature measurements at 40 K with electro-optical sampling at a wavelength of 890 nm show a FWHM of 1 ps. [less ▲]

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See detailArray Processing in Correlated Noise Fields Based on Instrumental Variables and Subspace Fitting
Viberg, Mats; Stoica, Petre; Ottersten, Björn UL

in IEEE Transactions on Signal Processing (1995), 43

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See detailPerformance Analysis of Direction Finding with Large Arrays and Finite Data
Viberg, Mats; Ottersten, Björn UL; Nehorai, A.

in IEEE Transactions on Signal Processing (1995)

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See detailPsychologische Trainigs für die Praxis - Darstellung zweier Verfahren
Steffgen, Georges UL

in Psychologie und Sport -Schorndorf- (1995), 2(3), 1-5

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See detailTargeting of Listeria monocytogenes ActA protein to the plasma membrane as a tool to dissect both actin-based cell morphogenesis and ActA function.
Friederich, Evelyne UL; Gouin, E.; Hellio, R. et al

in The EMBO journal (1995), 14(12), 2731-44

Actin assembly on the surface of Listeria monocytogenes in the cytoplasm of infected cells provides a model to study actin-based motility and changes in cell shape. We have shown previously that the ActA ... [more ▼]

Actin assembly on the surface of Listeria monocytogenes in the cytoplasm of infected cells provides a model to study actin-based motility and changes in cell shape. We have shown previously that the ActA protein, exposed on the bacterial surface, is required for polarized nucleation of actin filaments. To investigate whether plasma membrane-associated ActA can control the organization of microfilaments and cell shape, variants of ActA, in which the bacterial membrane signal had been replaced by a plasma membrane anchor sequence, were produced in mammalian cells. While both cytoplasmic and membrane-bound forms of ActA increased the F-actin content, only membrane-associated ActA caused the formation of plasma membrane extensions. This finding suggests that ActA acts as an actin filament nucleator and shows that permanent association with the inner face of the plasma membrane is required for changes in cell shape. Based on the observation that the amino-terminal segment of ActA and the remaining portion which includes the proline-rich repeats cause distinct phenotypic modifications in transfected cells, we propose a model in which two functional domains of ActA cooperate in the nucleation and dynamic turnover of actin filaments. The present approach is a new model system to dissect the mechanism of action of ActA and to further investigate interactions of the plasma membrane and the actin cytoskeleton during dynamic changes of cell shape. [less ▲]

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See detailFast and sensitive search of information databases for biological relationships
Schneider, Reinhard UL; Casari, G.; Sander, C.

in Statustagung des BMBF, HPSC 95, Stand und Perspektiven des Parallelen Höchstleistungsrechnens und seiner Anwendungen (1995)

Sequence comparison has become an essential and standard tool in the analysis of genomic data. Genome projects will decipher much of the genetic information in many organisms, including humans. As a ... [more ▼]

Sequence comparison has become an essential and standard tool in the analysis of genomic data. Genome projects will decipher much of the genetic information in many organisms, including humans. As a result, the computational cost of databank searches will increase dramatically. In addition, the search for biologically meaningful homology between a newly determined sequence and sequences already stored in the various databanks becomes increasingly important as most of the new data will be in raw, not understood form. The detection of sufficient similarity between a newly determined sequence to a protein of know function or even known 3D-structure in a databank allows one to transfer most of the knowledge from one sequence to the other. The result can be enormous savings in genetic and biochemical laboratory efforts. [less ▲]

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See detailKleist speichern. Zur Verzeichnung des handschriftlichen Nachlasses von Georg Minde-Pouet
Amann, Wilhelm UL; Wangermann, Tobias

in Brandenburger Kleist-Blätter (1995), 8

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See detailS.A.S. : un exemple à suivre ?
Corbisier, Isabelle UL; Nicaise, Pierre

in Le droit des affaires = Het ondernemingsrecht (1994), (33), 43-80

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See detailInduction of c-Jun and suppresion of CREB transmission factor proteins in axotomized neurons of substantia nigra and covariation with tyrosine hydroxilase
Brecht, S; Gass, P; Anton, Fernand UL et al

in Molecular and Cellular Neuroscience (1994), 5(5), 431-441

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See detailPouvoir princier et peuplement : aux origines de la ville de Luxembourg
Margue, Michel UL

in Château Gaillard (1994), 16

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See detailDetection of atmospheric pressure loading using the Global Positioning System
van Dam, Tonie UL; Blewitt, Geoffrey; Heflin, M.

in Journal of Geophysical Research (1994), 99(B12), 23939-23950

Earth deformation signals caused by atmospheric pressure loading are detected in vertical position estimates at Global Positioning System (GPS) stations. Surface displacements due to changes in ... [more ▼]

Earth deformation signals caused by atmospheric pressure loading are detected in vertical position estimates at Global Positioning System (GPS) stations. Surface displacements due to changes in atmospheric pressure account for up to 24% of the total variance in the GPS height estimates. The detected loading signals are larger at higher latitudes where pressure variations are greatest; the largest effect is observed at Fairbanks, Alaska (latitude 65°), with a signal RMS of 5 mm. Out of 19 continuously operating GPS sites (with a mean of 281 daily solutions per site), 18 show a positive correlation between the GPS vertical estimates and the modeled loading displacements. Accounting for loading reduces the variance of the vertical station positions on 12 of the 19 sites investigated. Removing the modeled pressure loading from GPS determinations of baseline length for baselines longer than 6000 km reduces the variance on 73 of the 117 baselines investigated. The slight increase in variance for some of the sites and baselines is consistent with expected statistical fluctuations. The results from most stations are consistent with ∼65% of the modeled pressure load being found in the GPS vertical position measurements. Removing an annual signal from both the measured heights and the modeled load time series leaves this value unchanged. The source of the remaining discrepancy between the modeled and observed loading signal may be the result of (1) anisotropic effects in the Earth's loading response, (2) errors in GPS estimates of tropospheric delay, (3) errors in the surface pressure data, or (4) annual signals in the time series of loading and station heights. In addition, we find that using site dependent coefficients, determined by fitting local pressure to the modeled radial displacements, reduces the variance of the measured station heights as well as or better than using the global convolution sum. [less ▲]

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See detailTeststatistische Überprüfung der Impact of Event-Skala: Befunde zu Reliabilität und Stabilität.
Ferring, Dieter UL; Filipp, Sigrun-Heide

in Focus Diagnostica (1994), 40(4)

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See detailCorrelated mutations and residue contacts in proteins
Göbel, U.; Sander, C.; Schneider, Reinhard UL et al

in Proteins (1994), 18

The maintenance of protein function and structure constrains the evolution of amino acid sequences. This fact can be exploited to interpret correlated mutations observed in a sequence family as an ... [more ▼]

The maintenance of protein function and structure constrains the evolution of amino acid sequences. This fact can be exploited to interpret correlated mutations observed in a sequence family as an indication of probable physical contact in three dimensions. Here we present a simple and general method to analyze correlations in mutational behavior between different positions in a multiple sequence alignment. We then use these correlations to predict contact maps for each of 11 protein families and compare the result with the contacts determined by crystallography. For the most strongly correlated residue pairs predicted to be in contact, the prediction accuracy ranges from 37 to 68% and the improvement ratio relative to a random prediction from 1.4 to 5.1. Predicted contact maps can be used as input for the calculation of protein tertiary structure, either from sequence information alone or in combination with experimental information. [less ▲]

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See detailDossier Dewey. Het ontwerp van een transactionele handelingspsychologie.
Biesta, Gert UL

in Psychologie & maatschappij (1994), 18(2), 123-140

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See detailPhosphorus Redistribution During the Formation of Buried CoSi2 Layers by Ion Beam Synthesis,
Schüppen, A.; Jebasinski, R.; Mantl, S. et al

in Nuclear Instruments and Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms (1994), 84

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See detailMorele opvoeding en morele ontwikkeling; inleiding op het thema.
Biesta, Gert UL; Wardekker, W.; Zwart-Woudstra, H.

in Comenius (1994), 14(1), 5-11

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See detail«Nos w nos z humorem»
Roelens, Nathalie UL

in Humor Europejski (1994)

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See detailTesticular self-examination: Attitudes and practices among young men in Europe
Wardle, Jane; Steptoe, Andrew; Burckhardt, Ruth et al

in Preventive Medicine (1994), 23

BACKGROUND: Testicular self examination (TSE) is recommended for the early detection of testicular cancer. Evidence from North America suggests there is only limited public awareness of its importance ... [more ▼]

BACKGROUND: Testicular self examination (TSE) is recommended for the early detection of testicular cancer. Evidence from North America suggests there is only limited public awareness of its importance among the young male population. Compliance with regular TSE is found in only a small minority of young men. Attitudes toward and practice of TSE have rarely been studied outside North America. METHOD: Attitudes to TSE were evaluated by questionnaire in a sample of 16,486 students. Frequency of TSE practice was reported by the 7,304 men in the sample. The data were collected as part of the European Health Behavior Survey, an international study on health beliefs and health behavior. RESULTS: Eighty-seven percent of men reported never having practiced TSE. Regular practice (monthly) was reported by only 3% of the sample, with another 10% reporting occasional TSE. Significant differences emerged between countries, ranging from 76% of German men to 98% of Icelandic men reporting no TSE. Men rated TSE as less important to health than women. Attitude toward TSE among men was a significant predictor of TSE practice. CONCLUSION: Both the low levels of TSE and the low ratings of the importance of TSE suggest that young men in Europe are unaware of the value of this comparatively simple method of early detection of cancer. If a highly educated population group in the "at risk" age category is not carrying out the recommendations, it is unlikely that there are higher levels of compliance in other groups. These results suggest an important role for health education in the early detection of testicular cancer. [less ▲]

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See detailThe role of Pax-1 in axial skeleton development.
Wallin, J.; Wilting, J.; Koseki, H. et al

in Development (1994), 120(5), 1109-21

Previous studies have identified a single amino-acid substitution in the transcriptional regulator Pax-1 as the cause of the mouse skeletal mutant undulated (un). To evaluate the role of Pax-1 in the ... [more ▼]

Previous studies have identified a single amino-acid substitution in the transcriptional regulator Pax-1 as the cause of the mouse skeletal mutant undulated (un). To evaluate the role of Pax-1 in the formation of the axial skeleton we have studied Pax-1 protein expression in early sclerotome cells and during subsequent embryonic development, and we have characterized the phenotype of three different Pax-1 mouse mutants, un, undulated-extensive (unex) and Undulated short-tail (Uns). In the Uns mutation the whole Pax-1 locus is deleted, resulting in the complete absence of Pax-1 protein in these mice. The other two genotypes are interpreted as hypomorphs. We conclude that Pax-1 is necessary for normal vertebral column formation along the entire axis, although the severity of the phenotype is strongest in the lumbar region and the tail. Pax-1-deficient mice lack vertebral bodies and intervertebral discs. The proximal part of the ribs and the rib homologues are also missing or severely malformed, whereas neural arches are nearly normal. Pax-1 is thus required for the development of the ventral parts of vertebrae. Embryonic analyses reveal that although sclerotomes are formed in mutant embryos, abnormalities can be detected from day 10.5 p.c. onwards. The phenotypic analyses also suggest that the notochord still influences vertebral body formation some days after the sclerotomes are formed. Furthermore, the notochord diameter is larger in mutant embryos from day 12 p.c., due to increased cell proliferation. In the strongly affected genotypes the notochord persists as a rod-like structure and the nucleus pulposus is never properly formed. Since the notochord is Pax-1-negative these findings suggest a bidirectional interaction between notochord and paraxial mesoderm. The availability of these Pax-1 mutant alleles permitted us to define an early role for Pax-1 in sclerotome patterning as well as a late role in intervertebral disc development. Our observations suggest that Pax-1 function is required for essential steps in ventral sclerotome differentiation, i.e. for the transition from the mesenchymal stage to the onset of chondrogenesis. [less ▲]

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See detailNADPH-diaphorase reactivity in articular afferents of a normal and inflamed knee joint in the cat.
Bscheidl, Christoph; Hanesch, Ulrike UL; Heppelmann, Bernd

in Brain Research (1994), 668

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See detailMouse genetics and the development of vertebral column development
Balling, Rudi UL; Koseki, H; Wallin, J et al

in Proceedings Greenwood Genetics Center (1994), (13), 58-60

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See detailNichts als Ärger. Zur Modifikation von Ärgerreaktionen im Leistungssport
Steffgen, Georges UL

in Leistungssport (1994), 24(3), 27-30

Ein Fußballspieler, der aufgrund seiner "Wut im Bauch" ein Revanchefoul begeht, oder ein anderer, der aufgrund seines Ärgers das Siegestor erzielt, sind Beispiele für höchst unterschiedliche Auswirkungen ... [more ▼]

Ein Fußballspieler, der aufgrund seiner "Wut im Bauch" ein Revanchefoul begeht, oder ein anderer, der aufgrund seines Ärgers das Siegestor erzielt, sind Beispiele für höchst unterschiedliche Auswirkungen der Emotion Ärger. Im Kontext leistungssportlichen Handelns wird von Sportlern, Trainern, Betreuern und Sportwissenschaftlern die leistungsfördernde Wirkung von Ärgererleben heftig diskutiert. Um die Kontroversen zu entschärfen, wird in diesem Beitrag eine psychologische Sichtweise zum Thema "Ärger" dargelegt. Die Gründe für eine Veränderung von Ärgerreaktionen, ein theoretischer Erklärungsansatz sowie ein Modifikationsprogramm des Ärgers werden hierzu vorgestellt. Ziel ist es, ein differenzierteres Bild der Veränderungsnotwendigkeiten und -möglichkeiten von Ärgerreaktionen aufzuzeigen. [less ▲]

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See detailGeschichte und gegenwärtiger Trend der Pestalozzi-Forschung in der Schweiz
Tröhler, Daniel UL

in Finnish Journal of Education (1994), 25(3), 321-324

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See detailInstitutionelle Unsicherheit und wirtschaftliche Entwicklung
Klump, Rainer UL; Reichel, Richard

in Jahrbucher für Nationalokonomie und Statistik (1994)

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See detailToeplitz quantization of Kähler manifolds and gl(N), N to infinity, limits
Bordemann, Martin UL; Meinrenken, Eckhard; Schlichenmaier, Martin UL

in Communications in Mathematical Physics (1994), 165

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See detailEducation as Practical Intersubjectivity. Towards a critical-pragmatic understanding of education.
Biesta, Gert UL

in Educational Theory (1994), 44(3), 299-317

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See detailMorele opvoeding en morele ontwikkeling
Wardekker,W.; Biesta, Gert UL; Tavecchio,L. et al

in Comenius (1994), 14(1), 5-82

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See detailFremdenfeindliche Gewalt: Tätertypen, Eskalationsprozesse und Erklärungsansätze
Willems, Helmut UL; Eckert, Roland

in Politisches Lernen (1994), (1-2), 11-25

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See detailRegulation of apoptosis in the immune system
Krammer, P. H.; Behrmann, Iris UL; Daniel, P. T. et al

in Current Opinion in Immunology (1994), 6(2), 279-89

Apoptosis in T and B lymphocytes is involved in all fundamental processes in the immune system. It is a mechanism to regulate the course of an immune response and to establish immunological memory as well ... [more ▼]

Apoptosis in T and B lymphocytes is involved in all fundamental processes in the immune system. It is a mechanism to regulate the course of an immune response and to establish immunological memory as well as central and peripheral tolerance. Apoptosis in lymphocytes is regulated by gene products that induce or block this process. Elucidating the molecular basis for sensitivity and resistance towards induction of apoptosis is the key to the understanding of the development of the immune system, basic immune reactions and the pathogenesis of autoimmune diseases, AIDS and cancer. [less ▲]

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See detailLe projet de dictionnaire biographique du mouvement ouvrier luxembourgeois
Scuto, Denis UL

in Matériaux pour l'histoire de notre temps (1994), (34), 47-49

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See detailPHD - AN AUTOMATIC MAIL SERVER FOR PROTEIN SECONDARY STRUCTURE PREDICTION
ROST, B.; SANDER, C.; Schneider, Reinhard UL

in Computer Applications in the Biosciences [=CABIOS] (1994), 10(1), 53-60

By the middle of 1993, > 30000 protein sequences had been listed. For 1000 of these, the three-dimensional (tertiary) structure has been experimentally solved. Another 7000 can be modelled by homology ... [more ▼]

By the middle of 1993, > 30000 protein sequences had been listed. For 1000 of these, the three-dimensional (tertiary) structure has been experimentally solved. Another 7000 can be modelled by homology. For the remaining 21000 sequences, secondary structure prediction provides a rough estimate of structural features. Predictions in three states range between 35% (random) and 88% (homology modelling) overall accuracy. Using information about evolutionary conservation as contained in multiple sequence alignments, the secondary structure of 4700 protein sequences was predicted by the automatic e-mail sewer PHD, For proteins with at least one known homologue, the method has an expected overall three-state accuracy of 71.4% for proteins with at least one known homologue (evaluated on 126 unique protein chains). [less ▲]

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See detailStadtlogistik - Interessen „statt Logistik“?
Hatzfeld, Ulrich; Hesse, Markus UL

in Internationales Verkehrswesen (1994), 46(11), 646-653

The paper was one of the early attempts to address and conceptualise inner-city distribution logistics (aka city logistics), that is, the attempt to introduce collaborative modes of goods delivery in ... [more ▼]

The paper was one of the early attempts to address and conceptualise inner-city distribution logistics (aka city logistics), that is, the attempt to introduce collaborative modes of goods delivery in urban areas. Different from classical transport studies and engineering approaches, this paper particularly emphasises the role of individual (stakeholder) interests, which represent a major determinant of (and often barrier to) any such collaborative policy schemes. Given the recent calls for introducing a renewed city logistics in the context of e-commerce and the associated rise of last-mile deliveries, it might be wise to include a related perspective on single and collective rationalities, aims and objectives. This was the main reason to bury the 25 yrs-old paper from the archives. [less ▲]

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See detailTHE HSSP DATABASE OF PROTEIN-STRUCTURE SEQUENCE ALIGNMENTS
SANDER, C.; Schneider, Reinhard UL

in Nucleic Acids Research (1994), 22(17), 3597-3599

HSSP (homology-derived structures of proteins) is a derived database merging structural (2-D and 3-D) and sequence information (1-D). For each protein of known 3D structure from the Protein Data Bank, the ... [more ▼]

HSSP (homology-derived structures of proteins) is a derived database merging structural (2-D and 3-D) and sequence information (1-D). For each protein of known 3D structure from the Protein Data Bank, the database has a file with all sequence homologues, properly aligned to the PDB protein. Homologues are very likely to have the same 3D structure as the PDB protein to which they have been aligned. As a result, the database is not only a database of sequence aligned sequence families, but it is also a database of implied secondary and tertiary structures. [less ▲]

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See detailRelationship between different approaches to the relativistic two body problem
del Sol Mesa, Antonio UL

in Journal of Physics: A Mathematical and General (1994)

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See detailPragmatism as a pedagogy of communicative action.
Biesta, Gert UL

in Studies in Philosophy & Education (1994), 13

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See detailInitial steps of myogenesis in somites are independent of influence from axial structures.
Bober, E.; Brand-Saberi, B.; Ebensperger, C. et al

in Development (1994), 120(11), 3073-82

Formation of paraxial muscles in vertebrate embryos depends upon interactions between early somites and the neural tube and notochord. Removal of both axial structures results in a complete loss of ... [more ▼]

Formation of paraxial muscles in vertebrate embryos depends upon interactions between early somites and the neural tube and notochord. Removal of both axial structures results in a complete loss of epaxial myotomal muscle, whereas hypaxial and limb muscles develop normally. We report that chicken embryos, after surgical removal of the neural tube at the level of the unsegmented paraxial mesoderm, start to develop myotomal cells that express transcripts for the muscle-specific regulators MyoD and myogenin. These cells also make desmin, indicating that the initial steps of axial skeletal muscle formation can occur in the absence of the neural tube. However, a few days following the extirpation, the expression of MyoD and myogenin transcripts gradually disappears, and becomes almost undetectable after 4 days. From these observations we conclude that the neural tube is not required for the generation of the skeletal muscle cell lineage, but may support the survival or maitenance of further differentiation of the myotomal cell compartment. Notochord transplanted medially or laterally to the unsegmented paraxial mesoderm leads to a ventralization of axial structures but does not entirely prevent the early appearance of myoblasts expressing MyoD transcripts. However, the additional notochord inhibits subsequent development and maturation of myotomes. Taken together, our data suggest that neural tube promotes, and notochord inhibits, the process of myogenesis in axial muscles at a developmental step following the initial expression of myogenic bHLH regulators. [less ▲]

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See detailDetection of atmospheric pressure loading using Very Long Baseline Interferometry measurements
van Dam, Tonie UL; Herring, T. A.

in Journal of Geophysical Research (1994), 99(B3), 4505-4518

Loading of the Earth by the temporal redistribution of global atmospheric mass is likely to displace the positions of geodetic monuments by tens of millimeters both vertically and horizontally. Estimates ... [more ▼]

Loading of the Earth by the temporal redistribution of global atmospheric mass is likely to displace the positions of geodetic monuments by tens of millimeters both vertically and horizontally. Estimates of these displacements are determined by convolving National Meteorological Center (NMC) global values of atmospheric surface pressure with Farrell's elastic Green's functions. An analysis of the distances between radio telescopes determined by very long baseline interferometry (VLBI) between 1984 and 1992 reveals that in many of the cases studied there is a significant contribution to baseline length change due to atmospheric pressure loading. Our analysis covers intersite distances of between 1000 and 10,000 km and is restricted to those baselines measured more than 100 times. Accounting for the load effects (after first removing a best fit slope) reduces the weighted root-mean-square (WRMS) scatter of the baseline length residuals on 11 of the 22 baselines investigated. The slight degradation observed in the WRMS scatter on the remaining baselines is largely consistent with the expected statistical fluctuations when a small correction is applied to a data set having a much larger random noise. The results from all baselines are consistent with ∼60% of the computed pressure contribution being present in the VLBI length determinations. Site dependent coefficients determined by fitting local pressure to the theoretical radial displacement are found to reproduce the deformation caused by the regional pressure to within 25% for most inland sites. The coefficients are less reliable at near coastal and island stations. [less ▲]

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See detailErnst Martin: Johann Kettiger und Johann Heinrich Pestalozzi. Zur Wirkungsgeschichte Pestalozzis
Tröhler, Daniel UL

in Paedagogica Historica (1994), XXX(2), 671-682

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See detailGute Noten. Der Schüler Kleist in den Aufzeichnungen des Carl Eduard Albanus
Amann, Wilhelm UL

in Brandenburger Kleist-Blätter (1994), 7

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See detailOn some aspects of a relativistic problem
del Sol Mesa, Antonio UL

in Canadian Journal of Physics (1994)

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See detailREDEFINING THE GOALS OF PROTEIN SECONDARY STRUCTURE PREDICTION
ROST, B.; SANDER, C.; Schneider, Reinhard UL

in Journal of Molecular Biology (1994), 235(1), 13-26

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See detailObservations - A propos de la réaction de M.O. Ralet à notre étude parue au n° 6634 (n° 6657)
Corbisier, Isabelle UL

in Revue Pratique des Sociétés Civiles et Commerciales (1994), (4), 469-473

Observations d'I. Corbisier en réponse à une réaction de O. Ralet à propos d'une étude précédemment publiée par la première citée et portant plus spécialement sur l'administrateur personne morale et le ... [more ▼]

Observations d'I. Corbisier en réponse à une réaction de O. Ralet à propos d'une étude précédemment publiée par la première citée et portant plus spécialement sur l'administrateur personne morale et le mandataire substitué (art. 1994 du Code civil) [less ▲]

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See detailObservations sous Cass., 20 janvier 1994 (n° 6653)
Corbisier, Isabelle UL

in Revue Pratique des Sociétés Civiles et Commerciales (1994), (4), 408-409

Commentaire de l'une des rares décisions précisant que la règle stipulant l'irrecevabilité de l'action en justice d'une société demeurée en défaut de procéder au dépôt de son acte constitutif au greffe du ... [more ▼]

Commentaire de l'une des rares décisions précisant que la règle stipulant l'irrecevabilité de l'action en justice d'une société demeurée en défaut de procéder au dépôt de son acte constitutif au greffe du tribunal compétent n'est pas d'ordre public [less ▲]

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See detailEffects of nisoldipine on endothelin-1- and angiotensin II-induced immediate/early gene expression and protein synthesis in adult rat ventricular cardiomyocytes.
Grohe, C.; Nouskas, J.; Vetter, H. et al

in Journal of cardiovascular pharmacology (1994), 24(1), 13-6

The cellular mechanisms by which dihydropyridine-type calcium antagonists lead to regression of hypertension-related cardiac hypertrophy have not been clarified. We previously showed that angiotensin II ... [more ▼]

The cellular mechanisms by which dihydropyridine-type calcium antagonists lead to regression of hypertension-related cardiac hypertrophy have not been clarified. We previously showed that angiotensin II (AII) and endothelin-1 (ET-1) induce protein synthesis in isolated adult rat cardiomyocytes, probably through protein kinase C (PKC) as second messenger and the gene product of the early growth response gene-1 (Egr-1) as third messenger. We now show that the dihydropyridine derivative nisoldipine inhibits AII- and ET-1-induced protein synthesis at low concentrations (IC50 7.5 nM for 0.1 microM ET). Induction of c-fos and Egr-1 mRNA by AII and ET was completely blocked by nisoldipine. Therefore, nisoldipine may influence the signal transduction pathway, i.e., through PKC. These results provide a potential pressure-independent mechanism by which nisoldipine may influence development of cardiac hypertrophy. [less ▲]

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