Intestinal-Cell Kinase and Juvenile Myoclonic Epilepsy.

in New England Journal of Medicine (2019), 380(16), 24

With regard to the article by Bailey et al. (March 15, 2018, issue) on the potential role of variants in the gene encoding intestinal cell kinase (ICK) in genetic generalized epilepsies, including ... [more ▼]

With regard to the article by Bailey et al. (March 15, 2018, issue) on the potential role of variants in the gene encoding intestinal cell kinase (ICK) in genetic generalized epilepsies, including juvenile myoclonic epilepsy: We attempted replication by rechecking for enrichment of ICK variants in two previously published analyses of mainly familial cases of genetic generalized epilepsy, which included a total of 1149 cases of genetic generalized epilepsy and 5911 ethnically matched controls. We analyzed the burden of single-gene rare variants with the use of whole exome sequencing data, applying population stratification and both sample and variant quality control. We found no evidence of an enrichment of ICK variants in genetic generalized epilepsies or juvenile myoclonic epilepsy. Specifically, we did not detect a nonsynonymous variant in 357 persons with juvenile myoclonic epilepsy at a minor allele frequency at or below 0.1%. Although we cannot exclude the possibility that ICK variants may be population-specific risk factors for juvenile myoclonic epilepsy, the lack of validation in our cohorts does not support a true disease association but rather suggests that the authors’ results may be due to chance, possibly owing to methodologic issues (see the Supplementary Appendix, available with the full text of this letter at NEJM.org). [less ▲]

Into the ground

in Arch + (2018), (231), 78-83

How the financialization of property markets and land use puts cities under pressure.

Into the Μaze of Legitimacy and Efficiency; the Single Resolution Board as a Necessity

in Dogaru, Tatiana-Camelia (Ed.) (Re)forming Public Administration Paradigms in Global Governance Context (2018)

Die Intonation final fallender Nuklei. Eine kontrastive Untersuchung zum Hamburgischen und Berlinischen

in Germanistische Linguistik (2001), 157-158

Intorno al-i concetto-i di datore di lavoro. A proposito di The Concept of The Employer di Jeremias Prassl

in Giornale di Diritto del Lavoro e di Relazioni Industriali (2016)

The Intra-Party Effects of Open List Design

Doctoral thesis (2021)

Intracellular metabolite profiling of platelets: evaluation of extraction processes and chromatographic strategies.

in Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences (2012), 898

An extraction method for intracellular metabolite profiling should ideally be able to recover the broadest possible range of metabolites present in a sample. However, the development of such methods is ... [more ▼]

An extraction method for intracellular metabolite profiling should ideally be able to recover the broadest possible range of metabolites present in a sample. However, the development of such methods is hampered by the diversity of the physico-chemical properties of metabolites as well as by the specific characteristics of samples and cells. In this study, we report the optimization of an UPLC-MS method for the metabolite analysis of platelet samples. The optimal analytical protocol was determined by testing seven different extraction methods as well as by employing two different LC-MS methods, in which the metabolites were separated by using hydrophilic interaction liquid chromatography (HILIC) and reversed phase liquid chromatography (RPLC). The optimal conditions were selected using the coverage of the platelets' metabolome, the response of the identified metabolites, the reproducibility of the analytical method, and the time of the analysis as main evaluation criteria. Our results show that methanol-water (7:3) extraction coupled with HILIC-MS method provides the best compromise, allowing identification of 107 metabolites in a platelet cell extract sample, 91% of them with a RSD% lower than 20. A higher number of metabolites could be detected when analyzing the platelet samples with two different LC-MS methods or when using complementary extraction methods in parallel. [less ▲]

Intractable conflicts in Africa: The international response to the Darfur and South Sudan crises

in Global Campus Human Rights Journal (2017), 1(2), 287-314

This article considers the intractable conflicts and human rightssituations in Darfur, Sudan and South Sudan, respectively, against theinternational responses they elicited. Intractable conflicts are ... [more ▼]

This article considers the intractable conflicts and human rightssituations in Darfur, Sudan and South Sudan, respectively, against theinternational responses they elicited. Intractable conflicts are conflicts that havelasted for a long time with resistance to settlement despite various attempts atintervention and conciliation. These conflicts from neighbouring nations haveboth elicited extensive engagement from the international and regionalcommunities but, while some clarity regarding the direction to be taken has beenachieved in the case of South Sudan, the situation in Darfur remains dire. Thearticle analyses the difference in the peace-building approaches in the twoconflicts and how these approaches have contributed to the different outcomes inDarfur and South Sudan. Following an exposition of intractability in theintroduction, the second section applies the factors identified to the case ofDarfur, confirming that this indeed is an intractable situation. It then considersthe international response to the conflict in Darfur and the mechanismsemployed by the global and the regional community in an attempt to addressthis conflict. The third section considers the situation in South Sudan and theinternational response, noting that efforts were led by the regional and sub-regional bodies, with the UN’s role being to complement these efforts. Themethodology employed is a comparative analysis, in which the internationaland regional legal and institutional responses to the crisis in South Sudan areanalysed with a view to identifying the lessons to be applied in addressing thesituation in Darfur, utilising theoretical and functional approaches to legal andpolitical interventions. The final section draws from the insights gained incomparing the international response in Darfur and South Sudan, and concludesby attempting to extract general principles about intractability and theeffectiveness of international responses to situations considered to be intractable,noting in particular the importance of regional and sub-regional bodies takingthe lead in efforts to resolve intractable conflicts. [less ▲]

Intragrain charge transport in kesterite thin films-Limits arising from carrier localization

in JOURNAL OF APPLIED PHYSICS (2016), 120(17),

Intragrain charge carrier mobilities measured by time-resolved terahertz spectroscopy in state of the art Cu2ZnSn(S,Se)(4) kesterite thin films are found to increase from 32 to 140 cm(2) V-1 s(-1) with ... [more ▼]

Intragrain charge carrier mobilities measured by time-resolved terahertz spectroscopy in state of the art Cu2ZnSn(S,Se)(4) kesterite thin films are found to increase from 32 to 140 cm(2) V-1 s(-1) with increasing Se content. The mobilities are limited by carrier localization on the nanometer-scale, which takes place within the first 2 ps after carrier excitation. The localization strength obtained from the Drude-Smith model is found to be independent of the excited photocarrier density. This is in accordance with bandgap fluctuations as a cause of the localized transport. Charge carrier localization is a general issue in the probed kesterite thin films, which were deposited by co-evaporation colloidal inks, and sputtering followed by annealing with varying Se/S contents and yield 4.9\%-10.0 efficiency in the completed device. (C) 2016 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). [less ▲]

Intraoperative discrimination of native meningioma and dura mater by Raman spectroscopy

in Scientific Reports (2021)