Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailExpressed protein profile of a Tectomicrobium and other microbial symbionts in the marine sponge Aplysina aerophoba as evidenced by metaproteomics.
Chaib De Mares, Maryam; Jimenez, Diego Javier; Palladino, Giorgia et al

in Scientific reports (2018), 8(1), 11795

Aplysina aerophoba is an emerging model marine sponge, with a well-characterized microbial community in terms of diversity and structure. However, little is known about the expressed functional ... [more ▼]

Aplysina aerophoba is an emerging model marine sponge, with a well-characterized microbial community in terms of diversity and structure. However, little is known about the expressed functional capabilities of its associated microbes. Here, we present the first metaproteomics-based study of the microbiome of A. aerophoba. We found that transport and degradation of halogenated and chloroaromatic compounds are common active processes in the sponge microbiomes. Our data further reveal that the highest number of proteins were affiliated to a sponge-associated Tectomicrobium, presumably from the family Entotheonellaceae, as well as to the well-known symbiont "Candidatus Synechococcus spongiarium", suggesting a high metabolic activity of these two microorganisms in situ. Evidence for nitric oxide (NO) conversion to nitrous oxide was consistently observed for Tectomicrobia across replicates, by production of the NorQ protein. Moreover, we found a potential energy-yielding pathway through CO oxidation by putative Chloroflexi bacteria. Finally, we observed expression of enzymes that may be involved in the transformation of chitin, glycoproteins, glycolipids and glucans into smaller molecules, consistent with glycosyl hydrolases predicted from analyses of the genomes of Poribacteria sponge symbionts. Thus, this study provides crucial links between expressed proteins and specific members of the A. aerophoba microbiome. [less ▲]

Detailed reference viewed: 90 (2 UL)
Full Text
Peer Reviewed
See detailExpressing Receipt-Freeness and Coercion-Resistance in Logics of Strategic Ability: Preliminary Attempt
Tabatabaei, Masoud UL; Jamroga, Wojciech UL; Ryan, Peter UL

in The International Workshop on AI for Privacy and Security (PrAISe), 2016. (2016)

Voting is a mechanism of utmost importance to social processes. In this paper, we focus on the strategic aspect of information security in voting procedures. We argue that the notions of receipt-freeness ... [more ▼]

Voting is a mechanism of utmost importance to social processes. In this paper, we focus on the strategic aspect of information security in voting procedures. We argue that the notions of receipt-freeness and coercion resistance are underpinned by existence (or nonexistence) of a suitable strategy for some participants of the voting process. In order to back the argument formally, we provide logical ``transcriptions'' of the informal intuitions behind coercion-related properties that can be found in the existing literature. The transcriptions are formulated in the modal game logic ATL*, well known in the area of multi-agent systems. [less ▲]

Detailed reference viewed: 114 (8 UL)
Peer Reviewed
See detailExpression and function of Pax 1 during development of the pectoral girdle.
Timmons, P. M.; Wallin, J.; Rigby, P. W. et al

in Development (1994), 120(10), 2773-85

Pax 1 is a member of the paired-box containing gene family. Expression has previously been observed in the developing sclerotomes and later in the anlagen of the intervertebral discs. Analysis of Pax 1 ... [more ▼]

Pax 1 is a member of the paired-box containing gene family. Expression has previously been observed in the developing sclerotomes and later in the anlagen of the intervertebral discs. Analysis of Pax 1-deficient undulated mice revealed an important role for this gene in the development of the axial skeleton, in which Pax 1 apparently functions as a mediator of notochordal signals during sclerotome differentiation. Here we demonstrate that Pax 1 is also transiently expressed in the developing limb buds. A comparative phenotypic analysis of different undulated alleles shows that this expression is of functional significance. In mice that are mutant for the Pax 1 gene severe developmental abnormalities are found in the pectoral girdle. These include fusions of skeletal elements which would normally remain separate, and failures in the differentiation of blastemas into cartilaginous structures. Although Pax 1 is also expressed in the developing hindlimb buds and Wolffian ridge, no malformations could be detected in the corresponding regions of Pax 1 mutant mice. These findings show that, in addition to its role in the developing vertebral column, Pax 1 has an important function in the development of parts of the appendicular skeleton. [less ▲]

Detailed reference viewed: 69 (1 UL)
Full Text
Peer Reviewed
See detailExpression and functional analyses of novel mutations of ATP-binding cassette transporter-1 in Japanese patients with high-density lipoprotein deficiency
Nishida, Y.; Hirano, K.; Tsukamoto, K. et al

in Biochemical and Biophysical Research Communications (2002), 290(2), 713-721

ATP-binding cassette transporter-1 (ABCA1) gene is mutated in patients with familial high-density lipoprotein deficiency (FHD). In order to know the molecular basis for FHD, we characterized three ... [more ▼]

ATP-binding cassette transporter-1 (ABCA1) gene is mutated in patients with familial high-density lipoprotein deficiency (FHD). In order to know the molecular basis for FHD, we characterized three different ABCA1 mutations associated with FHD (G1158A/A255T, C5946T/R1851X, and A5226G/N1611D) with respect to their expression in the passaged fibroblasts from the patients and in the cells transfected with themutated cDNAs. Fibroblasts from the all patients showed markedly decreased cholesterol efflux to apolipoprotein (apo)-Al. In the fibroblasts homozygous for G1158A/A255T, the immunoreactive mass of ABCA1 could not be detected, even when stimulated by 9-cisretinoic acid and 22-R- hydroxycholesterol. In the fibroblasts homozygous for C5946T/R1851X, ABCA1 mRNA was comparable. Because the mutant ABCA1 protein (R1851X) was predicted to lack the epitope for the antibody used, we transfected FLAG-tagged truncated mutant (R1851X/ABCA1-FLAG) cDNA into Cos-7 cells, showing that the mutant protein expression was markedly reduced. The expression of N1611D ABCA1 protein was comparable in both fibroblasts and overexpressing cells, although cholesterol efflux from the cells was markedly reduced. These data indicated that, in the three patients investigated, the abnormalities and dysfunction of ABCA1 occurred at the different levels, providing important information about the expression, regulation, and function of ABCA1. [less ▲]

Detailed reference viewed: 93 (0 UL)
Peer Reviewed
See detailExpression of avian Pax1 and Pax9 is intrinsically regulated in the pharyngeal endoderm, but depends on environmental influences in the paraxial mesoderm.
Muller, Tim UL; Ebensperger, C.; Neubuser, A. et al

in Developmental Biology (1996), 178(2), 403-17

Pax1 and Pax9 represent a subfamily of paired-box-containing genes. In vertebrates, Pax1 and Pax9 transcripts have been found specifically in mesodermal tissues and the pharyngeal endoderm. Pax1 ... [more ▼]

Pax1 and Pax9 represent a subfamily of paired-box-containing genes. In vertebrates, Pax1 and Pax9 transcripts have been found specifically in mesodermal tissues and the pharyngeal endoderm. Pax1 expression in the sclerotomes has been shown to be indispensable for proper formation of the axial skeleton, but expression of Pax1 in the endoderm has not been studied in detail. We have cloned the chick homologue of the murine Pax9 gene. Our results show that transcripts of Pax1 and Pax9 are first detectable in the prospective foregut endoderm of headfold-stage avian embryos. Endodermal expression correlates with the highly proliferative zones of the folding foregut and evaginating pharyngeal pouches. In later stages, Pax1 and Pax9 are expressed in overlapping but distinct patterns within the developing sclerotomes and limb buds. From grafting experiments we conclude that activation of pharyngeal Pax1 and Pax9 expression is an intrinsic property of the endoderm, not requiring midline structures or head mesoderm. In contrast, notochord is required to induce Pax1 in competent sclerotomes. Here we show that in vitro there is a cranio-caudal gradient of inductive capacity in the notochord. This coincides with the graded expression of Pax1 and Pax9 along the cranio-caudal axis in 2- to 3-day-old embryos. Furthermore, paraxial head mesoderm shows no competence to express Pax1. Finally, in vitro we find counteracting influences on notochord signaling by lateral tissues (lateral plate, intermediate mesoderm), leading to an inhibition of Sonic hedgehog (Shh) expression in notochord and floor plate, as well as Pax1 and Pax9 expression in sclerotomes. Taken together, our results demonstrate that different mechanisms regulate expression of Pax1 and Pax9 in foregut and sclerotome, but suggest a common function for both genes in the two tissues that is promoting proliferation and preventing fusion of neighboring blastemas. [less ▲]

Detailed reference viewed: 106 (0 UL)
Full Text
Peer Reviewed
See detailExpression of the plasma membrane Ca2+-ATPase in myogenic cells.
Hammes, A.; Oberdorf-Maass, S.; Jenatschke, S. et al

in The Journal of biological chemistry (1996), 271(48), 30816-22

To study the physiological function of the plasma membrane calmodulin-dependent calcium ATPase (PMCA) in intact cells, L6 myogenic cell lines stably overexpressing the human PMCA isoform 4CI (= human PMCA ... [more ▼]

To study the physiological function of the plasma membrane calmodulin-dependent calcium ATPase (PMCA) in intact cells, L6 myogenic cell lines stably overexpressing the human PMCA isoform 4CI (= human PMCA isoform 4b) were generated. Several independent L6 clones and controls stably transfected with the empty expression vector were analyzed in detail. The resting cytosolic calcium level in hPMCA4CI-overexpressing muscle cells (measured by the Fura-2 method) was significantly reduced by 20-30% compared with controls. This was shown in a cytosolic window of 1322 single cells (p < 0.01). Furthermore, the differentiation process of these cells was remarkably accelerated compared with control myoblasts and parental nontransfected L6 cells as assessed by multinucleated myotube formation and creatine phosphokinase activity elevation. After 4 and 6 days of differentiation, PMCA-overexpressing L6 cells from four independent clones displayed a 3- and 4-fold higher creatine phosphokinase activity compared with controls (n = 5, p < 0.02). These results may extend the concept of the function of the PMCA from simple prevention of calcium overload to an active involvement in intracellular calcium regulation with potentially important consequences for cellular functions. [less ▲]

Detailed reference viewed: 106 (0 UL)
Full Text
Peer Reviewed
See detailExpression pattern suggests a role of MiR399 in the regulation of the cellular response to local Pi increase during arbuscular mycorrhizal symbiosis.
Branscheid, Anja; Sieh, Daniela; Pant, Bikram Datt et al

in Molecular plant-microbe interactions : MPMI (2010), 23(7), 915-26

Many plants improve their phosphate (Pi) availability by forming mutualistic associations with arbuscular mycorrhizal (AM) fungi. Pi-repleted plants are much less colonized by AM fungi than Pi-depleted ... [more ▼]

Many plants improve their phosphate (Pi) availability by forming mutualistic associations with arbuscular mycorrhizal (AM) fungi. Pi-repleted plants are much less colonized by AM fungi than Pi-depleted plants. This indicates a link between plant Pi signaling and AM development. MicroRNAs (miR) of the 399 family are systemic Pi-starvation signals important for maintenance of Pi homeostasis in Arabidopsis thaliana and might also qualify as signals regulating AM development in response to Pi availability. MiR399 could either represent the systemic low-Pi signal promoting or required for AM formation or they could act as counter players of systemic Pi-availability signals that suppress AM symbiosis. To test either of these assumptions, we analyzed the miR399 family in the AM-capable plant model Medicago truncatula and could experimentally confirm 10 novel MIR399 genes in this species. Pi-depleted plants showed increased expression of mature miR399 and multiple pri-miR399, and unexpectedly, levels of five of the 15 pri-miR399 species were higher in leaves of mycorrhizal plants than in leaves of nonmycorrhizal plants. Compared with nonmycorrhizal Pi-depleted roots, mycorrhizal roots of Pi-depleted M. truncatula and tobacco plants had increased Pi contents due to symbiotic Pi uptake but displayed higher mature miR399 levels. Expression levels of MtPho2 remained low and PHO2-dependent Pi-stress marker transcript levels remained high in these mycorrhizal roots. Hence, an AM symbiosis-related signal appears to increase miR399 expression and decrease PHO2 activity. MiR399 overexpression in tobacco suggested that miR399 alone is not sufficient to improve mycorrhizal colonization supporting the assumption that, in mycorrhizal roots, increased miR399 are necessary to keep the MtPho2 expression and activity low, which would otherwise increase in response to symbiotic Pi uptake. [less ▲]

Detailed reference viewed: 106 (1 UL)
Full Text
Peer Reviewed
See detailExpression patterns of cardiac aging in Drosophila.
Cannon, Leah; Zambon, Alexander C.; Cammarato, Anthony et al

in Aging cell (2017), 16(1), 82-92

Aging causes cardiac dysfunction, often leading to heart failure and death. The molecular basis of age-associated changes in cardiac structure and function is largely unknown. The fruit fly, Drosophila ... [more ▼]

Aging causes cardiac dysfunction, often leading to heart failure and death. The molecular basis of age-associated changes in cardiac structure and function is largely unknown. The fruit fly, Drosophila melanogaster, is well-suited to investigate the genetics of cardiac aging. Flies age rapidly over the course of weeks, benefit from many tools to easily manipulate their genome, and their heart has significant genetic and phenotypic similarities to the human heart. Here, we performed a cardiac-specific gene expression study on aging Drosophila and carried out a comparative meta-analysis with published rodent data. Pathway level transcriptome comparisons suggest that age-related, extra-cellular matrix remodeling and alterations in mitochondrial metabolism, protein handling, and contractile functions are conserved between Drosophila and rodent hearts. However, expression of only a few individual genes similarly changed over time between and even within species. We also examined gene expression in single fly hearts and found significant variability as has been reported in rodents. We propose that individuals may arrive at similar cardiac aging phenotypes via dissimilar transcriptional changes, including those in transcription factors and micro-RNAs. Finally, our data suggest the transcription factor Odd-skipped, which is essential for normal heart development, is also a crucial regulator of cardiac aging. [less ▲]

Detailed reference viewed: 82 (14 UL)
Peer Reviewed
See detailExpression patterns of L-plastin isoform in normal and carcinomatous breast tissues.
Lapillonne, A.; Coue, O.; Friederich, Evelyne UL et al

in Anticancer research (2000), 20(5A), 3177-82

Plastins are members of a family of actin-binding proteins which exhibit a tissue-specific expression pattern. L-plastin, which is specifically expressed in hematopoietic cell lineage, has been proposed ... [more ▼]

Plastins are members of a family of actin-binding proteins which exhibit a tissue-specific expression pattern. L-plastin, which is specifically expressed in hematopoietic cell lineage, has been proposed to be involved in the control of cell adhesion and motility. This protein is also frequently expressed in cell lines derived from mammary solid tumors and therefore might be involved in cancer invasion and metastasis. We have analysed plastin expression in normal and carcinomatous breast tissues in vivo by immunohistochemistry and immunoblotting approaches using specific plastin isoform antibodies. L-plastin was not detected in normal epithelial cells of the mammary gland whereas a staining of myoepithelial cells was observed in 50% of the cases. In breast carcinomas, a significant immunostaining of malignant epithelial cells was observed in 4 of the 29 cases analysed (13.8%). No correlation between L-plastin expression and tumor size, histological grade or lymph node status was observed. In contrast, L-plastin was found expressed in 4 of the 11 estrogen and progesterone receptors negative tumors (p = 0.039). The potential role of plastin expression in the tumor process is discussed. [less ▲]

Detailed reference viewed: 48 (1 UL)
Full Text
See detailExpressionistische Alpen-Passion. Pazifistische Idealisierung der Schweiz bei Yvan Goll und anderen
Wiegmann, Eva UL

in Gätje, Hermann; Singh, Sikander (Eds.) Konjunktionen. Yvan Goll im Diskurs der Moderne (2017)

Detailed reference viewed: 103 (5 UL)
Full Text
See detailExpressionistische Lyrik als Kulturpolitik
Dembeck, Till UL

in Lubkoll, Christine; Illi, Manuel; Hampel, Anna (Eds.) Politische Literatur. Begriffe – Debatten – Aktualität (2018)

Detailed reference viewed: 56 (0 UL)
Full Text
See detailExpressions & relations mathématiques élémentaires
Korvers, Stéphane UL

Learning material (2016)

Ce texte introduit les outils de base des mathématiques que sont les expressions algébriques et numériques. Les nombres réels, les opérations sur les nombres, les moyennes, les sommes partielles de ... [more ▼]

Ce texte introduit les outils de base des mathématiques que sont les expressions algébriques et numériques. Les nombres réels, les opérations sur les nombres, les moyennes, les sommes partielles de termes de suites particulières, les polynômes ou la factorisation des expressions algébriques sont à compter parmi les thèmes abordés. La structure des notes vise à accompagner le lecteur au travers de ces éléments théoriques et à lui permettre de développer ses compétences mathématiques par la réalisation des nombreux exercices proposés. [less ▲]

Detailed reference viewed: 62 (4 UL)
Full Text
Peer Reviewed
See detailExpressive incoherence and alexithymia in autism spectrum disorder
Pinto Costa, Andreia UL; Steffgen, Georges UL; Samson, Andrea C.

in Journal of Autism and Developmental Disorders (2017)

Expressive incoherence can be implicated in socio-emotional communicative problems in autism spectrum disorder (ASD). The present study examined expressive incoherence in 37 children with ASD and 41 ... [more ▼]

Expressive incoherence can be implicated in socio-emotional communicative problems in autism spectrum disorder (ASD). The present study examined expressive incoherence in 37 children with ASD and 41 typically developing (TD) children aged 3–13 years old during a frustration task. The role of alexithymia in expressive incoherence was also assessed. Compared to TD children, children with ASD had higher expressive incoherence, such as more neutral and positive emotion expressions during negative behaviors, but not in the expression of negative emotions during positive behaviors. Further analyses revealed that alexithymia moderated the expressions of positive emotions during negative behaviors. These results suggest that children with ASD may benefit from interventions targeting alexithymia to increase emotional coherence, which may improve socio-emotional communication. [less ▲]

Detailed reference viewed: 115 (6 UL)
Full Text
Peer Reviewed
See detailExpressiveness modulo Bisimilarity of Regular Expressions with Parallel Composition
Baeten, J.; Luttik, B.; Muller, Tim UL et al

in Proc. 17th International Workshop on Expressiveness in Concurrency (2010)

The languages accepted by finite automata are precisely the languages denoted by regular expressions. In contrast, finite automata may exhibit behaviours that cannot be described by regular expressions up ... [more ▼]

The languages accepted by finite automata are precisely the languages denoted by regular expressions. In contrast, finite automata may exhibit behaviours that cannot be described by regular expressions up to bisimilarity. In this paper, we consider extensions of the theory of regular expressions with various forms of parallel composition and study the effect on expressiveness. First we prove that adding pure interleaving to the theory of regular expressions strictly increases its expressiveness up to bisimilarity. Then, we prove that replacing the operation for pure interleaving by ACP-style parallel composition gives a further increase in expressiveness. Finally, we prove that the theory of regular expressions with ACP-style parallel composition and encapsulation is expressive enough to express all finite automata up to bisimilarity. Our results extend the expressiveness results obtained by Bergstra, Bethke and Ponse for process algebras with (the binary variant of) Kleene's star operation. [less ▲]

Detailed reference viewed: 86 (0 UL)
Full Text
Peer Reviewed
See detailExtend the Concept of Public Key Encryption with Delegated Search
Tang, Qiang UL; Ma, Hua; Chen, Xiaofeng

in Computer Journal (2015), 58(4), 11

We revisit the concept of public key encryption with delegated keyword search (PKEDS), a concept proposed by Ibraimi et al. A PKEDS scheme allows a receiver to authorize a third-party server to search in ... [more ▼]

We revisit the concept of public key encryption with delegated keyword search (PKEDS), a concept proposed by Ibraimi et al. A PKEDS scheme allows a receiver to authorize a third-party server to search in two ways: either according to a message chosen by the server itself or according to a trapdoor sent by the receiver.We first show some observations on the primitive formulation and the proposed PKEDS scheme by Ibraimi et al. and point out that the single-server setting may limit the application of this primitive. We then extend the concept to the multiple-server setting and present a new security model. We finally propose a new PKEDS scheme, which is proven secure, and compare it with that by Ibraimi et al. [less ▲]

Detailed reference viewed: 120 (2 UL)
Full Text
Peer Reviewed
See detailExtended Adjacency and Scale-dependent Graph Fourier Transform via Diffusion Distances
Elias, Vitor R.M.; Alves Martins, Wallace UL; Werner, Stefan

in IEEE Transactions on Signal and Information Processing over Networks (2020)

This paper proposes the augmentation of the adjacency model of networks for graph signal processing. It is assumed that no information about the network is available, apart from the initial adjacency ... [more ▼]

This paper proposes the augmentation of the adjacency model of networks for graph signal processing. It is assumed that no information about the network is available, apart from the initial adjacency matrix. In the proposed model, additional edges are created according to a Markov relation imposed between nodes. This information is incorporated into the extended-adjacency matrix as a function of the diffusion distance between nodes. The diffusion distance measures similarities between nodes at a certain diffusion scale or time, and is a metric adopted from diffusion maps. Similarly, the proposed extended-adjacency matrix depends on the diffusion scale, which enables the definition of a scale-dependent graph Fourier transform. We conduct theoretical analyses of both the extended adjacency and the corresponding graph Fourier transform and show that different diffusion scales lead to different graph-frequency perspectives. At different scales, the transform discriminates shifted ranges of signal variations across the graph, revealing more information on the graph signal when compared to traditional approaches. The scale-dependent graph Fourier transform is applied for anomaly detection and is shown to outperform the conventional graph Fourier transform. [less ▲]

Detailed reference viewed: 158 (7 UL)