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See detailProduction of bio-hydrogen and methane during semi-continuous digestion of maize silage in a two-stage system
Benito Martin, Patricia C.; Greger, Manfred UL; Schlienz, Markus UL

in International Journal of Hydrogen Energy (2017), 42

The feasibility and performance of applying a two-stage configuration for co-production of hydrogen and methane from maize silage in continuously stirred reactors was investigated under mesophilic ... [more ▼]

The feasibility and performance of applying a two-stage configuration for co-production of hydrogen and methane from maize silage in continuously stirred reactors was investigated under mesophilic conditions. The high organic loading used in the first-stage hydrogen producing reactor (e.g. load shock treatment) was effective at ensuring hydrogen-producing conditions, with no methanogenic activity observed for more than 60 days. A hydrogen yield of up to 53.8 NlH2/kg volatile solid (VS) was measured in the first reactor, with a hydrogen content of 33.1%. The methane yield in the second stage reactor was 133.9 NlCH4/kgVS, with a methane content of 65%. Abnormally low concentration of acetic acid and high concentrations of caproic acid were measured in the first reactor in the pH range 5–5.5, which could be explained by the presence of strains such as Clostridium kluyveri. Of the estimated total energy yield in the two-stage system, only 4% was from hydrogen production. The mixture of hydrogen and methane produced in the system (after carbon dioxide removal) is in the range recommended for use as vehicle fuel. [less ▲]

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See detailAn in silico re-design of the metabolism in Thermotoga maritima for increased biohydrogen production
Nogales, Juan E; Gudmunsson, Steinn; Thiele, Ines UL

in International Journal of Hydrogen Energy (2012), 37(17), 1220512218

Microbial hydrogen production is currently hampered by lack of efficiency. We examine how hydrogen production in the hyperthermophilic bacterium Thermotoga maritima can be increased in silico. An updated ... [more ▼]

Microbial hydrogen production is currently hampered by lack of efficiency. We examine how hydrogen production in the hyperthermophilic bacterium Thermotoga maritima can be increased in silico. An updated genome-scale metabolic model of T. maritima was used to i) describe in detail the H2 metabolism in this bacterium, ii) identify suitable carbon sources for enhancing H2 production, and iii) to design knockout strains, which increased the in silico hydrogen production up to 20%. A novel synthetic oxidative module was further designed, which connects the cellular NADPH and ferredoxin pools by inserting into the model a NADPH-ferredoxin reductase. We then combined this in silico knock-in strain with a knockout strain design, resulting in an in silico production strain with a predicted 125% increase in hydrogen yield. The in silico strains designs presented here may serve as blueprints for future metabolic engineering efforts of T. maritima. [less ▲]

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