References of "Biomedical Optics Express"
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See detailDextran adsorption onto red blood cells revisited: single cell quantification by laser tweezers combined with microfluidics
Lee, K.; Shirshin, E.; Rovnyagina, N. et al

in Biomedical Optics Express (2018)

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See detailReal-time optical pH measurement in a standard microfluidic cell culture system
Magnusson, E. B.; Halldorsson, S.; Fleming, Ronan MT UL et al

in Biomedical Optics Express (2013), 4(9), 1749-1758

The rapid growth of microfluidic cell culturing in biological and biomedical research and industry calls for fast, non-invasive and reliable methods of evaluating conditions such as pH inside a ... [more ▼]

The rapid growth of microfluidic cell culturing in biological and biomedical research and industry calls for fast, non-invasive and reliable methods of evaluating conditions such as pH inside a microfluidic system. We show that by careful calibration it is possible to measure pH within microfluidic chambers with high accuracy and precision, using a direct single-pass measurement of light absorption in a commercially available phenol-red-containing cell culture medium. The measurement is carried out using a standard laboratory microscope and, contrary to previously reported methods, requires no modification of the microfluidic device design. We demonstrate the validity of this method by measuring absorption of light transmitted through 30-micrometer thick microfluidic chambers, using an inverted microscope fitted with a scientific-grade digital camera and two bandpass filters. In the pH range of 7\&\#x02013;8, our measurements have a standard deviation and absolute error below 0.05 for a measurement volume smaller than 4 nL. [less ▲]

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