![]() Lommel, Maiti ![]() ![]() ![]() in FASEB Journal (2016) Deregulated cell migration and invasion are hallmarks of metastatic cancer cells. Phosphorylation on residue Ser5 of the actin-bundling protein L-plastin activates L-plastin and has been reported to be ... [more ▼] Deregulated cell migration and invasion are hallmarks of metastatic cancer cells. Phosphorylation on residue Ser5 of the actin-bundling protein L-plastin activates L-plastin and has been reported to be crucial for invasion and metastasis. Here, we investigate signal transduction leading to L-plastin Ser5 phosphorylation using 4 human breast cancer cell lines. Whole-genome microarray analysis comparing cell lines with different invasive capacities and corresponding variations in L-plastin Ser5 phosphorylation level revealed that genes of the ERK/MAPK pathway are differentially expressed. It is noteworthy that in vitro kinase assays showed that ERK/MAPK pathway downstream ribosomal protein S6 kinases α-1 (RSK1) and α-3 (RSK2) are able to directly phosphorylate L-plastin on Ser5. Small interfering RNA- or short hairpin RNA-mediated knockdown and activation/inhibition studies followed by immunoblot analysis and computational modeling confirmed that ribosomal S6 kinase (RSK) is an essential activator of L-plastin. Migration and invasion assays showed that RSK knockdown led to a decrease of up to 30% of migration and invasion of MDA-MB-435S cells. Although the presence of L-plastin was not necessary for migration/invasion of these cells, immunofluorescence assays illustrated RSK-dependent recruitment of Ser5-phosphorylated L-plastin to migratory structures. Altogether, we provide evidence that the ERK/MAPK pathway is involved in L-plastin Ser5 phosphorylation in breast cancer cells with RSK1 and RSK2 kinases able to directly phosphorylate L-plastin residue Ser5. [less ▲] Detailed reference viewed: 248 (17 UL)![]() Küpper, Achim ![]() in Jahrbuch der Deutschen Schillergesellschaft (2016), 60 Detailed reference viewed: 119 (0 UL)![]() Küpper, Achim ![]() in Germanistische Mitteilungen (2016), 42(1), 73-83 Detailed reference viewed: 66 (1 UL)![]() ; ; van der Torre, Leon ![]() in Deontic Logic and Normative Systems (2016) (2016) Deontic logic sentences define what an agent ought to do when faced with a set of norms. These norms may come into conflict such that a priority ordering over them is necessary to resolve these conflicts ... [more ▼] Deontic logic sentences define what an agent ought to do when faced with a set of norms. These norms may come into conflict such that a priority ordering over them is necessary to resolve these conflicts. Dung's seminal paper raised the - so far open - challenge of how to use formal argumentation to represent non monotonic logics, highlighting argumentation's value in exchanging, communicating and resolving possibly conflicting viewpoints in distributed scenarios. In this paper, we propose a formal framework to study various properties of prioritized non monotonic reasoning in formal argumentation, in line with this idea. More precisely, we show how a version of prioritized default logic and Brewka-Eiter's construction in answer set programming can be obtained in argumentation via the weakest and last link principles. We also show how to represent Hansen's recent construction for prioritized normative reasoning by adding arguments using weak contraposition via permissive norms, and their relationship to Caminada's "hang yourself" arguments. [less ▲] Detailed reference viewed: 121 (10 UL)![]() Hofmann, Herwig ![]() in Auby, Jean-Bernard; Perroud, Thomas (Eds.) Droit compare de la procedure administrative (2016) Detailed reference viewed: 146 (1 UL)![]() Hofmann, Herwig ![]() ![]() in Bandi, Gula (Ed.) Reports of the 2016 FIDE conference (2016) Detailed reference viewed: 165 (8 UL)![]() Hofmann, Herwig ![]() in Harlow, Carol; dela Canaea, Giacinto; Sandberg, Lino (Eds.) Research Handbook in EU Administrative Law (2016) Detailed reference viewed: 262 (4 UL)![]() Hofmann, Herwig ![]() in Schneider, Jens-Peter; Rennert, Klaus; Marsch, Nikolaus (Eds.) Musterentwurf für ein EU-Verwaltungsverfahrensrecht (2016) Detailed reference viewed: 117 (5 UL)![]() Hofmann, Herwig ![]() Book published by Oxford University Press (2016) Detailed reference viewed: 230 (8 UL)![]() Hofmann, Herwig ![]() Book published by Bruylandt (2016) Detailed reference viewed: 115 (3 UL)![]() ; ; et al in Workshop on Ontology and Semantic Web Patterns, Kobe 18 October 2016 (2016) In this paper we present an ontology design pattern to conceptualize complaints - an important domain still uncovered by ODPs. The proposed Complaint Ontology Pattern (COP) has been designed based on the ... [more ▼] In this paper we present an ontology design pattern to conceptualize complaints - an important domain still uncovered by ODPs. The proposed Complaint Ontology Pattern (COP) has been designed based on the analysis of free text complaints from available complaint datasets (banking, air transport, automobile) among other knowledge sources. We present a detailed use case from consumer disputes. We evaluate the pattern by annotating the complaints from our use case and by discussing how COP aligns to existing ontologies. [less ▲] Detailed reference viewed: 125 (5 UL)![]() Chauvel, Louis ![]() in Georges, Nathalie; Schronen, Danielle; Urbé, Robert (Eds.) Sozialalmanach Inegalitéiten 2016 (2016) The international debate on NEETS differs from Luxembourguish realities: the problem associated with unemployment or downward mobile youth as it is described in Japan, Australia or France has no perfect ... [more ▼] The international debate on NEETS differs from Luxembourguish realities: the problem associated with unemployment or downward mobile youth as it is described in Japan, Australia or France has no perfect equivalent in Luxembourg, where the main problem of youth integration is housing shortage and increasing difficulties to gain autonomy, issues that are exacerbated for the children of immigrants. [less ▲] Detailed reference viewed: 423 (46 UL)![]() Heimböckel, Dieter ![]() ![]() in ZiG (2016), 7(2), Detailed reference viewed: 98 (3 UL)![]() Rodopoulos, Ioannis ![]() in La Revue des Droits de l'Homme (2016) Detailed reference viewed: 127 (2 UL)![]() Moreno Grandas, Diana Paola ![]() in Artificial Intelligence for Engineering Design, Analysis and Manufacturing – AIEDAM (2016), 20(2), 185-199 Design fixation is a phenomenon with important significance to many fields of design due to the potential negative impacts it may have in design outcomes, especially during the ideation stage of the ... [more ▼] Design fixation is a phenomenon with important significance to many fields of design due to the potential negative impacts it may have in design outcomes, especially during the ideation stage of the design process. The present study aims to provide a framework for understanding, or at least probing, design fixation by presenting a review of existing defixation approaches, as well as metrics that have been employed to understand and account for design fixation. This study also describes the results of two design by analogy (DbA) methods, WordTree and SCAMPER, to overcome design fixation in an experiment that involved 97 knowledge-domain experts. The study outcomes are at least twofold: a common framework of metrics and approaches to overcome design fixation in a wide range of design problems and nonintuitive results for DbA approaches in design fixation and other related creativity metrics. The application of WordTree and SCAMPER shows that both methods yield increased novelty compared to a control, where the SCAMPER results are significantly higher than WordTree. It is also found that WordTree mitigates design fixation whereas SCAMPER appears to be ineffective for this purpose but effective to generate an increased quantity of novel ideas. These results demonstrate that both DbA methods provide defixation capabilities and enhance designers’ creativity during idea generation. [less ▲] Detailed reference viewed: 166 (0 UL)![]() ; ; et al in Clinical Neurophysiology (2016) OBJECTIVE: The pathophysiology of deep brain stimulation mechanisms and resistant freezing phenomena in idiopathic Parkinson's disease (iPD) remains incompletely understood. Further studies on the ... [more ▼] OBJECTIVE: The pathophysiology of deep brain stimulation mechanisms and resistant freezing phenomena in idiopathic Parkinson's disease (iPD) remains incompletely understood. Further studies on the neuromuscular substrates are needed. METHODS: We analyzed 16 patients with advanced iPD and bilateral subthalamic nucleus stimulation, and 13 age- and gender-matched healthy controls. Patients were tested after overnight withdrawal of medication with 'stimulation off' (StimOff) and 'stimulation on' (StimOn). Subjects performed continuous tapping of the right index finger with simultaneous recordings of biomechanical registration, EMG of finger flexors and extensors, and EEG. First, we analyzed EEG and EMG spectral measures comparing StimOff with healthy controls and StimOff with StimOn (irrespective of freezing). Second, we contrasted 'regular (unimpaired) tapping' and 'freezing' resistant to subthalamic neurostimulation as obtained in StimOn. RESULTS: iPD showed increased intermuscular coherence around 8Hz in StimOff that was reduced in StimOn. This 8Hz muscular activity was not coherent to cortical activity. 'Freezing' episodes showed increased muscle activity of finger flexors and extensors at 6-9Hz, and increased cortical activity at 7-11Hz. During transition from regular tapping to 'freezing' the cortical activity first increased over the left sensorimotor area followed by a spread to the left frontal and right parietal areas. CONCLUSIONS: We identified neuromuscular motor network features of subthalamic neurostimulation therapy and resistant upper limb freezing that point to increased low-frequency muscular and cortical activity. SIGNIFICANCE: Together, our findings demonstrate several motor network abnormalities associated with upper limb freezing that may translate into future research on freezing of gait in iPD. [less ▲] Detailed reference viewed: 186 (13 UL)![]() ; ; et al in Clinical Neurophysiology (2016), 127(6), 2386-93 Detailed reference viewed: 176 (10 UL)![]() Ligeti, Katalin ![]() ![]() in Durdevic, Zlata; Ivicevic Karas, Elizabeta (Eds.) European Criminal Procedure Law in Service of Protection of European Union Financial Interests: State of Play and Challeges (2016) Detailed reference viewed: 402 (18 UL)![]() Machado Carneiro, Joël ![]() Presentation (2016) Detailed reference viewed: 91 (0 UL)![]() Codeca, Lara ![]() ![]() ![]() in IEEE Intelligent Transportation Systems Magazine (2016) Both the industrial and the scientific communities are working on problems related to vehicular traffic congestion, intelligent transportation systems, and mobility patterns using information collected ... [more ▼] Both the industrial and the scientific communities are working on problems related to vehicular traffic congestion, intelligent transportation systems, and mobility patterns using information collected from a variety of sources. Usually, a vehicular traffic simulator, with an appropriate scenario for the problem at hand, is used to reproduce realistic mobility patterns. Many mobility simulators are available, and the choice is made based on the type of simulation required, but a common problem is finding a realistic traffic scenario. The aim of this work is to provide and evaluate a scenario able to meet all the basic requirements in terms of size, realism, and duration, in order to have a common basis for evaluations. In the interest of building a realistic scenario, we used information from a real city with a typical topology common in mid-size European cities, and realistic traffic demand and mobility patterns. In this paper, we show the process used to build the Luxembourg SUMO Traffic (LuST) Scenario, and present a summary of its characteristics together with our evaluation and validation of the traffic demand and mobility patterns. [less ▲] Detailed reference viewed: 435 (52 UL) |
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