Une plateforme collaborative et ouverte de testing assisté par ordinateur permettant l'élaboration et la passation d'instruments d'évlauation par voie d'Internet 18e Colloque international de l'ADMEE-Europe:"Comment évaluer?Outils,dispositifs et acteurs"

in Actes du 18e colloque de l’ADMEE-Europe Reims 2005: Comment évaluer? Outils, dispositifs, acteurs. (2005)

Etiology of Obesity

in Munsch, S.; Beglinger, P. (Eds.) Obesity and Binge Eating Disorder (2005)

Interleukin-8 primes oxidative burst in neutrophil-like HL-60 through changes in cytosolic calcium

in Cell Calcium (2005), 37(6), 531-40

In response to a variety of stimuli, neutrophils release large amount of reactive oxygen species (ROS) generated by NADPH oxidase. This process known as the respiratory burst is dependent on cytosolic ... [more ▼]

In response to a variety of stimuli, neutrophils release large amount of reactive oxygen species (ROS) generated by NADPH oxidase. This process known as the respiratory burst is dependent on cytosolic free calcium concentration ([Ca(2+)](i)). Proinflammatory cytokines such as interleukin-8 (IL-8) may modulate ROS generation through a priming phenomenon. The aim of this study was to determine the effect of human IL-8 on ROS production in neutrophil-like dimethylsulfoxide-differentiated HL-60 cells (not equalHL-60 cells) and further to examine the role of Ca(2+) mobilization during the priming. IL-8 at 10 nM induced no ROS production but a [Ca(2+)](i) rise (254 +/- 36 nM). IL-8 induced a strongly enhanced (2 fold) ROS release during stimulation with 1 microM of N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLF). This potentiation of ROS production is dependent of extracellular Ca(2+) (17.0+/-4.5 arbitrary units (A.U.) in the absence of Ca(2+) versus 56.6 +/- 3.9 A.U. in the presence of 1.25 mM of Ca(2+)). Also, IL-8 enhanced fMLF-stimulated increase in [Ca(2+)](i) (375 +/- 35 versus 245 +/- 21 nM, 0.1 microM of fMLF). IL-8 had no effect on not equalHL-60 cells in response to 1 microM of thapsigargin (472 +/- 66 versus 470 +/- 60 nM). In conclusion, Ca(2+) influx is necessary for a full induction of neutrophil priming by IL-8. [less ▲]

Education

in Kerr; Moretti, M.; Weitkunat, R. (Eds.) ABC of Behaviour Change (2005)

Elemente der Konfrontationsbehandlung im Ernährungsmanagement und beim Umgang mit Heißhungerattacken bei Frauen mit Bulimia nervosa

in Neudeck, P.; Wittchen, H.-U. (Eds.) Reader Konfrontationstherapie (2005)

International Humanitarian Law, War Criminality and Child Recruitment

in Leiden Journal of International Law (2005), 18

Die "gespaltene Stadt" - Sozialräumliche Differenzierung und die Probleme benachteiligter Wohngebiete

in Heitmeyer, Wilhelm; Imbusch, Peter (Eds.) Integrationspotenziale einer modernen Gesellschaft (2005)

Geschichte und Sprache der Pädagogik

in Zeitschrift für Pädagogik (2005), 51

Charisma, grammar of schooling und Steuerungsprobleme des Bildungssystems Pestalozzi und sein Institut in Yverdon

in Göhlich, Michael; Hopf, Caroline; Sausele, Ines (Eds.) Pädagogische Organisationsforschung (2005)

Beyond annotation transfer by homology: novel protein-function prediction methods to assist drug discovery

in Drug Discovery Today (2005), 10(21), 1475-1482

Every entirely sequenced genome reveals 100s to 1000s of protein sequences for which the only annotation available is 'hypothetical protein'. Thus, in the human genome and in the genomes of pathogenic ... [more ▼]

Every entirely sequenced genome reveals 100s to 1000s of protein sequences for which the only annotation available is 'hypothetical protein'. Thus, in the human genome and in the genomes of pathogenic agents there could be 1000s of potential, unexplored drug targets. Computational prediction of protein function can play a role in studying these targets. We shall review the challenges, research approaches and recently developed tools in the field of computational function-prediction and we will discuss the ways these issues can change the process of drug discovery. [less ▲]